Control mechanism on structure, composition, and calcification of dentin and odontoblasts by time information transmitter melatonin

• Project/Area Number: 15K11034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tsurumi University (2017); Kochi Gakuen College (2015-2016)
• Principal Investigator: MISHIMA Hiroyuki 鶴見大学, 歯学部, 非常勤講師 (30112957)
• Co-Investigator(Kenkyū-buntansha): 見明 康雄 東京歯科大学, 歯学部, 准教授 (00157421) ; 鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476) ; 服部 淳彦 東京医科歯科大学, 教養部, 教授 (70183910) ; 池亀 美華 岡山大学, 医歯薬学総合研究科, 准教授 (70282986) ; 筧 光夫 明海大学, 歯学部, 講師 (90095331)
• Project Period (FY): 2015-10-21 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: メラトニン / 象牙質 / 象牙芽細胞 / アパタイト結晶 / メラトニン受容体 / 石灰化機構 / 石灰化球 / コラーゲン線維 / 石灰化度 / 石灰化 / 顕微ラマン分析 / 偏光顕微鏡 / MALDI TOF-MS法 / 成長線

Outline of Final Research Achievements

The expression of MT1 and MT2 melatonin receptor was confirmed in the odontoblasts of the control group. In addition, the expression of MT1 was stronger than that of MT2. A strong expression of MT1 was detected in the odontoblasts in the melatonin-treated groups. In the melatonin-treated groups, the height of odontoblasts was high and the number of odontoblasts increased. The number and size of calcospherites in predentin increased in proportion to the concentration of melatonin. The crystallinity and orientation of apatite crystal in dentin were improved by administration of melatonin. The odontoblasts were activated by melatonin, and the secretion of collagen fibers was promoted. Melatonin administration changed the behavior of Ca and P concentration in serum at night. Melatonin is efficiently incorporated into odontoblasts. The present study suggests that melatonin changed the blood composition in the body and influenced the structure, calcification, and crystallinity of dentin.

Research Products

• [Journal Article] a-melanocyte-stimulating hormone promotes the bone resorption resulting from increased osteoblastic and osteoclastic activities in goldfish. (2018)
  • Author(s): Ishizu, H., Sekiguchi, T., Ikari, T.,, Kitamura, K., Kitani, Y., Endo, M., Urata, M., Kinoshita, Y., Hattori, A., Srivastav, A.K., Mishima, H., Mizusawa, K., Takahashi, A. and Suzuki, N.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 262 Pages: 99-105
  • DOI: 10.1016/j.ygcen.2018.03.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b> (2017)
  • Author(s): Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura KI, Endo M, Kobayashi I, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N.
  • Journal Title: Biomedical Research Volume: 38 Issue: 1 Pages: 71-77
  • DOI: 10.2220/biomedres.38.71
  • NAID: 130005242919
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nature of Apatite Crystals in the Tooth of <i>Eusthenopteron</i> from Devonian (2017)
  • Author(s): Mishima H, Kakei M, Sasagawa I, Miake Y
  • Journal Title: Journal of Hard Tissue Biology Volume: 26 Issue: 4 Pages: 399-404
  • DOI: 10.2485/jhtb.26.399
  • NAID: 130006832128
  • ISSN: 1341-7649, 1880-828X
  • Peer Reviewed / Open Access
• [Journal Article] 天然アパタイトの組成と生体応用の可能性 (2017)
  • Author(s): 見明康雄、三島弘幸
  • Journal Title: 歯科学報 Volume: 117 Pages: 441-443
  • Open Access
• [Journal Article] Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctata (2017)
  • Author(s): Masayuki Sato, Koji Yachiguchi, Keiichi Motohashi, Yukio Yaguchi, Yoshiaki Tabuchi, Yoichiro Kitani,, Takahiro Ikari, Shouzo Ogiso, Toshio Sekiguchi, Tran Ngoc Hai, Do Thi Thanh Huong, Nguyen Viet Hoang, Makoto Urata, Hiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki
  • Journal Title: Fish Sci Volume: ー Issue: 4 Pages: 543-550
  • DOI: 10.1007/s12562-017-1086-0
  • NAID: 120006306228
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Aspects of the apatite crystal: Two pathways for apatite formation, the mechanisms underlying crystal structure defects, and the pathological calcification event (2016)
  • Author(s): Kakei M, Yoshikawa M, Sakae T, Mishima H
  • Journal Title: 化石研究会会誌 Volume: 48 Pages: 53-65
  • NAID: 40020817723
  • Peer Reviewed
• [Journal Article] Sodium fluoride influences on bone metabolism in goldfish: Analysis by scale osteoblasts and osteoclasts (2016)
  • Author(s): Sato M, Hanemoto T, Yachiguchi K, Tabuchi Y, Kondo T, Endo M, Kitani Y, Sekiguchi T, Uraka M, Mishima H, Hattori A, Suzuki N
  • Journal Title: 2016 Joint Seminar on Environmantal Ecology and Restoration between Taiwan and Japan Proceedings Volume: ー Pages: 53-55
• [Journal Article] 生体リズム同調因子メラトニンが象牙芽細胞，象牙質の構造及び石灰化機構に及ぼす影響 (2016)
  • Author(s): 三島弘幸、武内昇平、田辺咲貴、服部淳彦、鈴木信雄、筧光夫、松本敬、池亀美華、武市和彦、見明康雄
  • Journal Title: 日本再生歯科医学会誌 Volume: 14 Pages: 5-17
  • NAID: 130005395190
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fluroide exposure may accelerate the osteoporotic change in postmenopausal women: animal model of fluroride-induced osteoporosis (2015)
  • Author(s): Kakei M, Yoshikawa M ,Mishima H
  • Journal Title: Advanced Techniques in Biology & Medicine Volume: 4 Issue: 01 Pages: 1-4
  • DOI: 10.4172/2379-1764.1000170
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel tryptophan derivatives as potentially effective therapeutic drugs to treat bone diseases. (2015)
  • Author(s): Suzuki, N., Somei, M., Seki, A., Sekiguchi, T., Tabuchi, Y., Mishima, H., Kase, Y., Kaminishi, A., Yachiguchi, K., Kitamura, K., Oshima, Y., Hayakawa, K., Yano, S. and Hattori, A
  • Journal Title: Am. J. Life Sci. Volume: 3 Issue: 3 Pages: 31-38
  • DOI: 10.11648/j.ajls.s.2015030302.16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 魚類のウロコを用いた宇宙生物学的研究：キンギョのウロコ及び骨疾患モデルラットの骨代謝に対するブロモメラトニンの新規作用 (2015)
  • Author(s): 鈴木信雄、矢野幸子、大森克徳、北村敬一郎、清水宣明、西内 巧、染井正徳、関口俊男、渡辺良成、池亀美華、近藤 隆、田渕圭章、鈴木 徹、遠藤雅人、竹内俊郎、江尻貞一、三島弘幸、嶋津 徹、関 あずさ、舟橋久幸、高垣裕子、笠原春夫、瀬 睦、田谷敏貴、長野慎太郎、宮下知之、服部淳彦
  • Journal Title: Space Utilization Res Volume: 29 Pages: 87-90
  • NAID: 120006830288
  • Int'l Joint Research
• [Journal Article] メラトニン投与における象牙質の組織構造、象牙芽細胞や成長線周期に及ぼす影響 (2015)
  • Author(s): 三島弘幸，尾&#152846;真帆、武内昇平、武市和彦、服部淳彦，鈴木信雄，筧光夫，松本敬，池亀美華，見明康雄
  • Journal Title: 日本再生歯科医学会誌 Volume: 12 Pages: 2-14
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] デボン紀肉鰭類Eusthenopteron foodi の歯の組織と歯の支持様式 (2018)
  • Author(s): 三島弘幸、見明康雄、笹川一郎、筧光夫
  • Organizer: 平成29年度高知大学海洋コア総合研究センター共同利用・共同研究発表会
• [Presentation] 象牙質や象牙質結晶に対するメラトニンの影響 (2018)
  • Author(s): 三島弘幸、服部淳彦、鈴木信雄、松本敬、池亀美華、見明康雄、松本由樹
  • Organizer: 第123回日本解剖学会総会・全国学術集会
• [Presentation] メラトニンによる象牙質や象牙芽細胞の組織構造への影響 (2017)
  • Author(s): 三島弘幸、田辺咲貴、服部淳彦、鈴木信雄、松本敬、池亀美華、見明康雄
  • Organizer: 日本解剖学会
  • Place of Presentation: 長崎県長崎市
  • Year and Date: 2017-03-28
• [Presentation] Low-intensity pulsed ultrasound moderately activates osteoclasts: Analysis with goldfish scales as a model of bone. (2017)
  • Author(s): Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura K, Endo M, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N
  • Organizer: 環日本海域環境研究センター国際シンポジウム
  • Place of Presentation: 石川県能登町
  • Year and Date: 2017-02-28
  • Int'l Joint Research
• [Presentation] 概日リズム同調因子メラトニンによる象牙質や象牙芽細胞の組織構造 (2017)
  • Author(s): 三島弘幸、田辺咲貴、服部淳彦、鈴木信雄、筧光夫、松本敬、池亀美華、見明康雄、松本由樹
  • Organizer: 第35回化石研究会総会・学術大会
• [Presentation] 天然アパタイト結晶と生体硬組織におけるアパタイト結晶の比較 (2017)
  • Author(s): 三島弘幸、見明康雄、松本由樹
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] Effect of circadian rhythm synchronous factor melatonin on structure, composition, and calcification of dentin and odontoblasts (2017)
  • Author(s): Mishima H, Tanabe S, Hattori A, Suzuki N, Kakei M, Matsumoto T, Ikegame M, Miake Y, Matsumoto Y
  • Organizer: Biomin14 (14th International Symposium on Biomineralization)
  • Int'l Joint Research
• [Presentation] 抉出根完成中切歯へRevascularization療法を行った１症例 (2017)
  • Author(s): 大久保賢亮、大久保厚司、三島弘幸、林義彦
  • Organizer: 日本再生歯科医学会
  • Place of Presentation: 北海道札幌市
• [Presentation] 顕微ラマン分光分析装置による天然アパタイト結晶と生体硬組織のアパタイト結晶の解析 (2017)
  • Author(s): 三島弘幸、見明康雄,松本由樹、寺林優、大久保厚司、新藤恵美
  • Organizer: 日本再生歯科医学会
  • Place of Presentation: 北海道札幌市
• [Presentation] Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone (2016)
  • Author(s): Hanmoto T, Tabuchi Y, Ikegame M, Kondo, T, Kitamura K, Endo M, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N
  • Organizer: 日本動物学会
  • Place of Presentation: 沖縄県宜野湾市
  • Year and Date: 2016-11-17
• [Presentation] 魚類の耳石化石に観察される成長線に関する形態学及び分析学的研究 (2016)
  • Author(s): 三島弘幸、近藤康生、見明康雄
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-08-24
• [Presentation] 時刻情報伝達物質メラトニンが象牙質の構造や石灰化機構に及ぼす影響 (2016)
  • Author(s): 三島弘幸、武内昇平、服部淳彦，鈴木信雄，筧光夫，松本敬，池亀美華，見明康雄
  • Organizer: 第121回日本解剖学会学術総会
  • Place of Presentation: 福島県郡山市
  • Year and Date: 2016-03-28
• [Presentation] 天然アパタイト結晶と生体硬組織のアパタイト結晶との比較―生体硬組織における標準試料の可能性を探る― (2016)
  • Author(s): 三島弘幸、見明康雄、松本由樹、寺林優、新藤恵美
  • Organizer: 平成27年度高知大学海洋コア総合研究センター共同利用・共同研究成果発表会
  • Place of Presentation: 高知県高知市
  • Year and Date: 2016-02-29
• [Presentation] Effect of melatonin on structure, composition, and calcification of dentin (2016)
  • Author(s): Mishima H, Takeuchi S, Takechi K, Hattori A, Suzuki N, Kakei M, Matumoto T, Ikegame M, Miake Y
  • Organizer: TMD2016
  • Place of Presentation: Porvoo, Finland
  • Int'l Joint Research
• [Presentation] Effects of sodium fluoride on calcium metabolism in goldfish, Carassius auratus (2016)
  • Author(s): Sato M, Hanmoto T, Yachiguchi K, Tabuchi Y, Kondo T, Endo M, Kitani Y, Sekiguchi T, Urata M, Srivastav AK, Mishima H, Hattori A, Suzuki N
  • Organizer: 日本動物学会
  • Place of Presentation: 沖縄県宜野湾市
  • Int'l Joint Research
• [Presentation] 生体リズム伝達物質であるメラトニンが象牙質の組織構造に及ぼす影響 (2015)
  • Author(s): 武内昇平、三島弘幸、服部淳彦、鈴木信雄、筧光男、松本敬、池亀美華、見明康雄
  • Organizer: 第10回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2015-12-06
• [Remarks] http://www.kochi-gc.ac.jp