Pathophysiological analysis of oral diseases caused by dysregulation of cation channels
| Project/Area Number |
15K11036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉田 卓史 東北大学, 歯学研究科, 助教 (30455795)
高橋 哲 東北大学, 歯学研究科, 教授 (60226850)
熊本 裕行 東北大学, 歯学研究科, 教授 (70215028)
窪田 寿彦 東北大学, 歯学研究科, 助教 (80377746)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | TRP / チャネル / 亜鉛 / 骨芽細胞 / カリウムチャネル / パッチクランプ法 / 亜鉛イオン / 骨芽細胞様細胞MC3T3-E1細胞 / 過分極 / K+チャネル |
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| Outline of Final Research Achievements |
We studied the effects of the stimulating ingredients on TRPA1 channel recombinantly expressed in HEK293 cells using the Ca2+ imaging analysis and the patch-clamp technique. Formaldehyde, butylaldehyde, methyl ethyl ketone, and NNK (4-(methylnitrosoamino) -1-(3-pyridyl)-1-butanoe) activated TRPA1 channel, although acetone, crotonaldyhyde, isoprene, and nicotine did not induce Ca2+ transient. Acrylonitrile induced TRPA1 channel current.
We examined the responses to the cigarette smoke elements in trigeminal ganglion neurons and human gingival fibroblast 1. TRPA1 channel was expressed in TRG neurons and HGF-1 cells. Similar to the selective TRPA1 agonist allyl isothiocyanate (AITC), the chemical substances contained in the smoke of tobacco increased intracellular Ca2+ concentration, and induced the outwardly rectifying currents. The responses were blocked by the selective TRPA1 antagonist HC-030031. Therefore it is suggested that TRPA1 channel is activated by the ingredients.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Pharmacological properties of SAK3, a novel T-type voltage-gated Ca2+ channel enhancer.2017
Author(s)
Yabuki Y, Matsuo K, Izumi H, Haga H, Yoshida T, Wakamori M, Kakehi A, Sakimura K, Fukuda T, Fukunaga K.
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Journal Title
Neuropharmacology.
Volume: 117
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Alternative splicing in the C-terminal tail of Cav2.1 is essential for preventing a neurological disease in mice.2017
Author(s)
Aikawa T, Watanabe T, Miyazaki T, Mikuni T, Wakamori M, Sakurai M, Aizawa H, Ishizu N, Watanabe M, Kano M, Mizusawa H, Watase K.
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Journal Title
Hum Mol Genet.
Volume: 26(16)
Pages: 3094-3104
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inhibition of veratridine-induced delayed inactivation of the voltage-sensitive sodium channel by synthetic analogs of crambescin B,2017
Author(s)
Tadaaki Tsukamoto, Yukie Chiba, Atsuo Nakazaki, Yuki Ishikawa, Yoshiki Nakane, Yuko Cho, Mari Yotsu-Yamashita, Toshio Nishikawa, Minoru Wakamori, and Keiichi Konoki,*
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Journal Title
Bioorganic & Medicinal Chemistry Letters
Volume: 27
Issue: 5
Pages: 1247-1251
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Compromised maturation of GABAergic inhibition underlies abnormal network activity in the hippocampus of epileptic Ca2+ channel mutant mice, tottering.2015
Author(s)
Nakao A, Miki T, Shimono K, Oka H, Numata T, Kiyonaka S, Matsushita K, Ogura H, Niidome T, Noebels JL, Wakamori M, Imoto K, Mori Y
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Journal Title
Pflugers Arch
Volume: 467
Issue: 4
Pages: 737-752
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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