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Mechanism and expression of CD302 as a new regulator of osteoclast maturation

Research Project

Project/Area Number 15K11038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

Aoyama Eriko  岡山大学, 医歯薬学総合研究科, 助教 (10432650)

Co-Investigator(Kenkyū-buntansha) 滝川 正春  岡山大学, 医歯薬学総合研究科, 教授 (20112063)
服部 高子  岡山大学, 医歯薬学総合研究科, 助教 (00228488)
西田 崇  岡山大学, 医歯薬学総合研究科, 准教授 (30322233)
久保田 聡  岡山大学, 医歯薬学総合研究科, 教授 (90221936)
Co-Investigator(Renkei-kenkyūsha) HOSHIJIMA Mitsuhiro  岡山大学, 医歯薬学総合研究科, 助教 (30736567)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords破骨細胞 / アクチンリング / CD302/DCL-1 / 骨吸収 / osteoclast / actin / actin ring / CCN2/CTGF / CCN 2/CTGF
Outline of Final Research Achievements

CD302/DCL-1 is one of the C-type lectin receptors, but the distribution and the function has been mostly not clarified. We found that CD302 was expressed on osteoclasts induced from murine bone marrow cells. The inhibition of CD302 expression caused fragmentation of actin ring in mature osteoclasts and reduced bone resorption in vitro. Also, CD302 was co-localized with CCN2 which is a positive regulator of osteoclast maturation. Moreover, SHP-2 was identified as a potent candidate as a signal transducer of CD302 signaling. These results showed that CD302 could be a new target protein to regulate osteoclast maturation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (32 results)

All 2017 2016 2015

All Journal Article (15 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 13 results,  Open Access: 8 results,  Acknowledgement Compliant: 7 results) Presentation (15 results) (of which Int'l Joint Research: 3 results) Book (2 results)

  • [Journal Article] UPR Transducer BBF2H7 Allows Export of Type II Collagen in a Cargo- and Developmental Stage-Specific Manner2017

    • Author(s)
      Tokiro Ishikawa, Takuya Toyama, Yuki Nakamura, Kentaro Tamada, Hitomi Shimizu, Satoshi Ninagawa, Tetsuya Okada, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Eriko Aoyama, Masaharu Takigawa, Akihiro Harada and Kazutoshi Mori
    • Journal Title

      Journal of cell biology

      Volume: 印刷中 Issue: 6 Pages: 1761-1774

    • DOI

      10.1083/jcb.201609100

    • NAID

      120006343784

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A tumor suppressor gene product, platelet-derived growth factor receptor-like protein controls chondrocyte proliferation and differentiation.2017

    • Author(s)
      Kawata K, Kubota S, Eguchi T, Aoyama, E, Moritani N, Oka M, Kawaki H, and Takigawa M.
    • Journal Title

      J Cell Biochem

      Volume: 118 Issue: 11 Pages: 4033-4044

    • DOI

      10.1002/jcb.26059

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Catabolic effects of FGF-1 on chondrocytes and its possible role in osteoarthritis.2017

    • Author(s)
      El-Seoudi A, Abd El Kader T, Nishida T, Eguchi T, Aoyama E, Takigawa M, Kubota S
    • Journal Title

      J Cell Commun Signal.

      Volume: in press Issue: 3 Pages: 255-263

    • DOI

      10.1007/s12079-017-0384-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage.2017

    • Author(s)
      Janune D, Abd El Kader T, Aoyama E, Nishida T, Tabata Y, Kubota S, Takigawa M
    • Journal Title

      J Bone Miner Metab.

      Volume: in press Issue: 6 Pages: 582-597

    • DOI

      10.1007/s00774-016-0793-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evaluation of Molecular Interaction between CCN2 Protein and Its Binding Partners by Surface Plasmon Resonance (SPR).2017

    • Author(s)
      Aoyama E, Takigawa M.
    • Journal Title

      Methods Mol Biol.

      Volume: 1489 Pages: 169-176

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Production of Recombinant CCN2 Protein in Escherichia coli.2017

    • Author(s)
      Aoyama E, Hattori T, Kubota S, Takigawa M.
    • Journal Title

      Methods Mol Biol.

      Volume: 1489 Pages: 77-84

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The Role of Sonic Hedgehog Signaling in Osteoclastogenesis and Jaw Bone Destruction.2016

    • Author(s)
      Shimo T, Matsumoto K, Takabatake K, Aoyama E, Takebe Y, Ibaragi S, Okui T, Kurio N, Takada H, Obata K, Pang P, Iwamoto M, Nagatsuka H, Sasaki A.
    • Journal Title

      PLoS One.

      Volume: 11(3) Issue: 3 Pages: e0151731-e0151731

    • DOI

      10.1371/journal.pone.0151731

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Involvement of multiple CCN family members in platelets that support regeneration of joint tissues.2016

    • Author(s)
      Hara, C., Kubota, S., Nishida, T., Hiasa, M., Hattori, T., Aoyama, E., Moriyama, Y., Kamioka, H., Takigawa, M.
    • Journal Title

      Mod. Rheumatol.

      Volume: なし Issue: 6 Pages: 1-10

    • DOI

      10.3109/14397595.2016.1155255

    • NAID

      120006358661

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage.2016

    • Author(s)
      Janune D, Abd El Kader T, Aoyama E, Nishida T, Tabata Y, Kubota S, Takigawa M.
    • Journal Title

      J Bone Miner Metab

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-β3 function2016

    • Author(s)
      Yoshioka Y, Ono M, Maeda A, Kilts TM, Hara ES, Khattab H, Ueda J, Aoyama E, Oohashi T, Takigawa M, Young MF, Kuboki T.
    • Journal Title

      Bone

      Volume: 83 Pages: 162-70

    • DOI

      10.1016/j.bone.2015.11.007

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of CCN2 in amino acid metabolism of chondrocytes2016

    • Author(s)
      Murase, Y,, Hattori, T., Aoyama, E., Nishida, T., Maeda-Uematsu, A., Kawaki, H., Lyons, K. M., Sasaki, A., Takigawa, M., Kubota, S
    • Journal Title

      J. Cell Biochem

      Volume: 117 Issue: 4 Pages: 927-937

    • DOI

      10.1002/jcb.25377

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Involvement of multiple CCN family members in platelets that support regeneration of joint tissues2016

    • Author(s)
      Hara, C., Kubota, S., Nishida, T., Hiasa, M., Hattori, T., Aoyama, E., Moriyama, Y., Kamioka, H., Takigawa, M
    • Journal Title

      Mod. Rheumatol

      Volume: In press

    • NAID

      120006358661

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physical interaction of CCN2 with diverse growth factors involved in chondrocyte differentiation during endochondral ossification2015

    • Author(s)
      Khattab HM, Aoyama E, Kubota S, Takigawa M
    • Journal Title

      J. Cell Commun. Signal.

      Volume: 未定 Issue: 3 Pages: 247-54

    • DOI

      10.1007/s12079-015-0290-x

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CCN2 enhances RANKL-induced osteoclast differentiation via direct binding to RANK and OPG2015

    • Author(s)
      Eriko Aoyama , Satoshi Kubota , Hany Mohamed Khattab , Takashi Nishida , Masaharu Takigawa
    • Journal Title

      Bone

      Volume: 73 Pages: 242-248

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CCN family protein 2 (CCN2) promotes the early differentiation, but inhibits the terminal differentiation of skeletal myoblasts2015

    • Author(s)
      Nishida T, Kubota S, Aoyama E, Janune D, Lyons KM, Takigawa M.
    • Journal Title

      J Biochem

      Volume: 157(2) Issue: 2 Pages: 91-100

    • DOI

      10.1093/jb/mvu056

    • NAID

      40020353020

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 低出力超音波パルス(LIPUS)刺激による破骨細胞形成の抑制2017

    • Author(s)
      青山絵理子、久保田聡、山中信康、滝川正春
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CCN2結合因子GDF5の軟骨細胞における作用の解明2017

    • Author(s)
      井田すみれ、青山絵理子、久保田聡、滝川正春
    • Organizer
      第9回日本CCNファミリー研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GDF5との結合を介したCCN2の軟骨分化促進作用2017

    • Author(s)
      井田すみれ、青山絵理子、久保田聡、滝川正春
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CCN2結合因子CD302による破骨細胞成熟促進作用とその機序の解析 Mechanism of action of CCN2 binding protein CD302 ion osteoclast maturation2016

    • Author(s)
      青山絵理子、久保田聡、滝川正春
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 破骨細胞におけるCCN2結合性アクチン骨格制御因子CD302の作用機序の解明2016

    • Author(s)
      青山絵理子、久保田聡、滝川正春
    • Organizer
      第58回 歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] The mechanism of osteoclast maturation mediated with CD302/DCL-1binding to CCN22016

    • Author(s)
      Eriko Aoyama, Satoshi Kubota, Masaharu Takigawa
    • Organizer
      第34回日本骨代謝学会学術集会 第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      Osaka
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of CCN2/CTGF-related CD302 in osteoclast maturation2016

    • Author(s)
      Eriko Aoyama, Satoshi Kubota, Masaharu Takigawa
    • Organizer
      94th General Session & Exhibition of the IADR
    • Place of Presentation
      Seul, Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel regulatory factor in osteoclastogenesis DCL-1/CD302: Significance of its binding to CCN2/CTGF2016

    • Author(s)
      Eriko Aoyama, Mitsuhiro Hoshijima, Satoshi Kubota, Masaharu Takigawa
    • Organizer
      ECTS2016
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2016-05-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] CCN2/CTGFとRANK/TRAILおよびosteoprotegerinとの分子間相互作用を介した破骨細胞形成促進機構2016

    • Author(s)
      青山絵理子、久保田聡、西田崇、滝川正春
    • Organizer
      第 33回 岡山免疫懇話会
    • Place of Presentation
      岡山
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
  • [Presentation] CCN2結合因子CD302の破骨細胞機能制御作用2016

    • Author(s)
      青山絵理子、久保田聡、滝川正春
    • Organizer
      第 8回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Related Report
      2016 Research-status Report
  • [Presentation] 成熟破骨細胞のアクチンリング形成におけるCD302の機能とCCN2による制御 Novel role of CD302 in actin ring formation in mature osteoclasts and its interaction with CCN22015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第88回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 破骨細胞分化における新規アクチン骨格制御因子としてのDCL-1/CD302の役割とCCN2との関連2015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第58回歯科基礎医学会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 新たな破骨細胞制御因子DCL-1/CD302の作用機構の解明とCCN2との関連。2015

    • Author(s)
      青山絵理子、服部高子、星島光博、久保田聡、滝川正春
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 破骨細胞の成熟を制御する新規膜タンパク質CD302/DCL-1の機能とCCN2との結合。2015

    • Author(s)
      青山絵理子、服部高子、星島光博、久保田聡、滝川正春
    • Organizer
      第6回骨バイオサイエンス研究会
    • Place of Presentation
      岡山
    • Year and Date
      2015-07-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 成熟破骨細胞の形成におけるCD302/DCL-1の新機能とCCN2との関連。2015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第1回日本骨免疫学会
    • Place of Presentation
      沖縄
    • Year and Date
      2015-06-30
    • Related Report
      2015 Research-status Report
  • [Book] Evaluation of molecular interaction between CCN family 2 protein and binding partners by surface plasmon resonance (SPR) in Method in Molecular Biology: CCN proteins2016

    • Author(s)
      Aoyama E., Takigawa M
    • Total Pages
      576
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Book] Production of recombinant CCN family2 protein in Escherichia. coli. in Method in Molecular Biology: CCN proteins2016

    • Author(s)
      Aoyama E., Hattori T., Kubota S., Takigawa M
    • Total Pages
      576
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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