Elucidation of the molecular basis of Staphylococcus aureus exclusion via autophagy
| Project/Area Number |
15K11039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kagoshima University (2016-2017)
Hiroshima University (2015) |
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Principal Investigator |
HARADA Kae 鹿児島大学, 医歯学域歯学系, 助教 (60432663)
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| Co-Investigator(Kenkyū-buntansha) |
兼松 隆 広島大学, 医歯薬保健学研究科(歯), 教授 (10264053)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オートファジー / 細菌感染 / 感染 / 細菌排除 |
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| Outline of Final Research Achievements |
Autophagy is an intrinsic host defense system that recognizes and eliminates invading bacterial pathogens. We have identified microtubule-associated protein 1 light chain 3 (LC3), a marker protein of autophagy, as a binding partner of phospholipase C-related catalytically inactive protein (PRIP). To disclose the molecular mechanisms of bacterial elimination by autophagic pathway, we here examined by using a Salmonella Typhimurium infection assay. In mouse embryonic fibroblasts (MEFs) infected with Salmonella, the number of intracellular Salmonella and Salmonella in LC3-positive autophagosome-like vacuoles (SCVs: Salmonella containing vacuoles) were both increased in Prip-knockout (KO) MEFs. In addition, we analyzed autophagic flux, and autophagic flux in Prip-KO MEFs was impaired. Altogether, impaired acidification in SCVs of Prip-KO MEFs may induce abnormality in antibacterial autophagy, resulting in remarkable Salmonella proliferation.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] General anesthetic actions on GABAA receptors in vivo are reduced in phospholipase C-related catalytically inactive protein knockout mice.2017
Author(s)
Hayashiuchi M, Kitayama T, Morita K, Yamawaki Y, Oue K, Yoshinaka T, Asano S, Harada K, Kang Y, Hirata M, Irifune M, Okada M, Kanematsu T.
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Journal Title
Journal of Anesthesia
Volume: in press
Issue: 4
Pages: 531-538
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phospholipase C-related catalytically inactive protein is a new modulator of thermogenesis promoted by β-adrenergic receptors in brown adipocytes2015
Author(s)
Kana Oue, Jun Zhang, Kae Harada-Hada, Satoshi Asano, Yosuke Yamawaki, Masaki Hayashiuchi, Hisako Furusho, Takashi Takata, Masahiro Irifune, Masato Hirata, Takashi Kanematsu
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Journal Title
Journal of Biological Chemistry
Volume: 291
Issue: 8
Pages: 4185-96
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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