Analysis of the sweet receptor as an energy sensor

• Project/Area Number: 15K11045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: Sanematsu Keisuke 九州大学, 歯学研究院, 助教 (70567502)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 味覚 / 甘味受容体 / ミラクリン / TAS1R2/TAS1R3 / 味覚修飾物質

Outline of Final Research Achievements

Sweet taste is mediated by the sweet taste receptor, TAS1R2 + TAS1R3, with multiple biding sites for structurally diverse chemical substances. Using the sweet receptor assay in heterologously transfected HEK293 cells, we examined the sweet-modifying effect of miraculin. As a result, intracellular acidification was found to be required for the effect of miraculin.

Research Products

• [Journal Article] Diurnal Variation of Sweet Taste Recognition Thresholds Is Absent in Overweight and Obese Humans. (2018)
  • Author(s): Sanematsu K, Nakamura Y, Nomura M, Shigemura N, Ninomiya Y.
  • Journal Title: Nutrients Volume: 10 Issue: 3 Pages: 297-297
  • DOI: 10.3390/nu10030297
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bitter Taste Responses of Gustducin-positive Taste Cells in Mouse Fungiform and Circumvallate Papillae (2018)
  • Author(s): Yoshida Ryusuke、Takai Shingo、Sanematsu Keisuke、Margolskee Robert F.、Shigemura Noriatsu、Ninomiya Yuzo
  • Journal Title: Neuroscience Volume: 369 Pages: 29-39
  • DOI: 10.1016/j.neuroscience.2017.10.047
  • NAID: 120006845804
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 甘味受容体と甘味修飾物質との結合特性 (2018)
  • Author(s): 實松敬介
  • Journal Title: 日本味と匂学会誌 Volume: 25 Pages: 7-12
  • NAID: 130007395572
  • Peer Reviewed
• [Journal Article] Leptin suppresses sweet taste responses of enteroendocrine STC-1 cells. (2016)
  • Author(s): Jyotaki M, Sanematsu K, Shigemura N, Yoshida R, Ninomiya Y.
  • Journal Title: Neuroscience. Volume: 332 Pages: 76-87
  • DOI: 10.1016/j.neuroscience.2016.06.036
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Intracellular acidification is required for full activation of the sweet taste receptor by miraculin. (2016)
  • Author(s): Sanematsu K, Kitagawa M, Yoshida R, Nirasawa S, Shigemura N, Ninomiya Y.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 22807-22807
  • DOI: 10.1038/srep22807
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ミラクリンの味覚修飾効果における甘味受容体の完全活性化には細胞内酸性化が関与する (2017)
  • Author(s): 實松敬介 ，北川昌幸，吉田竜介 ，韮澤悟，重村憲徳，二ノ宮裕三
  • Organizer: 第94回日本生理学会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] 甘味受容体と甘味修飾物質との結合特性 (2017)
  • Author(s): 實松敬介
  • Organizer: 日本味と匂学会第51回大会
  • Invited
• [Presentation] Intracellular acidification is implicated in full activation of the sweet taste receptor in taste-modifying effect of miraculin (2016)
  • Author(s): Sanematsu K, Kitagawa M, Yoshida R, Nirasawa S, Shigemura N, Ninomiya Y.
  • Organizer: ISMNTOP2016
  • Place of Presentation: 福岡
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Responsiveness of gustducin-expressing taste cells to multiple bitter compounds (2016)
  • Author(s): Yoshida R, Takai S, Sanematsu K, Margolskee RF, Shigemura N, Ninomiya Y
  • Organizer: ISMNTOP2016
  • Place of Presentation: 福岡
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] 甘味受容体TAS1R2/TAS1R3 におけるギムネマ酸の甘味抑制効果の分子メカ ニズム (2016)
  • Author(s): 實松敬介
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-08-24
  • Invited
• [Presentation] ミラクリンによる甘味誘導効果の分子メカニズム (2016)
  • Author(s): 實松敬介，吉田竜介，重村憲徳，二ノ宮裕三
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-08-24
• [Presentation] Intracellular acidification is involved in full activation of the sweet taste receptor by miraculin (2016)
  • Author(s): Sanematsu K, Kitagawa M, Yoshida R, Nirasawa S, Shigemura N, Ninomiya Y.
  • Organizer: 17th International Symposium on Olfaction and Taste JASTS 50 th Annual Meeting
  • Place of Presentation: 横浜
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] Gustatory responses of taste receptor cells expressing fluorescent proteins in transgenic mice (2016)
  • Author(s): Yoshida R, Yasumatsu-Nakano K, Sanematsu K, Shigemura N, Ninomiya Y.
  • Organizer: 17th International Symposium on Olfaction and Taste JASTS 50 th Annual Meeting
  • Place of Presentation: 横浜
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] 甘味受容体T1R2/T1R3に対する味覚修飾作用の解析 (2015)
  • Author(s): 實松敬介、重村憲徳、二ノ宮裕三
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Book] 医学のあゆみ 各論：GPCR機能の新展開 【感覚】 味覚受容体 (2016)
  • Author(s): 實松敬介、二ノ宮裕三
  • Total Pages: 6
  • Publisher: 医歯薬出版