| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Regulator of membrane trafficking play an important role in osteoclastogenesis and bone resorption. We analyzed expression and role of Nedd4 adaptor protein in differentiation and function of osteoclast. Nedd4 adaptor protein was induced in mononuclear osteoclast precursor cells by RANKL via p38MAPK pathway. Nedd4 adaptor protein was also expressed in osteoclasts formed on dentin slices. In addition, expression of the protein was detected in osteoclasts of bone tissues of adjuvant-induced arthritis rat. Knockdown experiment with shRNA lentivirus revealed that Nedd4 adaptor protein regulates transport of RANK to cell surface in osteoclast precursor cells. In addition, analysis of osteoclasts derived from knockout mice which were prepared using the CRISPR/Cas9 system showed that Nedd4 adaptor protein regulates bone resorption of osteoclasts.
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