Novel function of osteoclastogenesis and bone resorption by membrane trafficking regulatory molecule
| Project/Area Number |
15K11046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Saga University |
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Principal Investigator |
Kukita Akiko 佐賀大学, 医学部, 准教授 (30153266)
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| Co-Investigator(Kenkyū-buntansha) |
久木田 敏夫 九州大学, 歯学研究院, 教授 (70150464)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 破骨細胞分化 / 小胞輸送 / 骨吸収 / 破骨細胞 / 分化 / 酸分泌 / 膜トラフィッキング / 膜タンパク質 |
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| Outline of Final Research Achievements |
Regulator of membrane trafficking play an important role in osteoclastogenesis and bone resorption. We analyzed expression and role of Nedd4 adaptor protein in differentiation and function of osteoclast. Nedd4 adaptor protein was induced in mononuclear osteoclast precursor cells by RANKL via p38MAPK pathway. Nedd4 adaptor protein was also expressed in osteoclasts formed on dentin slices. In addition, expression of the protein was detected in osteoclasts of bone tissues of adjuvant-induced arthritis rat. Knockdown experiment with shRNA lentivirus revealed that Nedd4 adaptor protein regulates transport of RANK to cell surface in osteoclast precursor cells. In addition, analysis of osteoclasts derived from knockout mice which were prepared using the CRISPR/Cas9 system showed that Nedd4 adaptor protein regulates bone resorption of osteoclasts.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] IL-1β induce pathologically activated osteoclasts bearing extremely high level of resorption activity imaged by pH-sensitive fluorescence probes: A possible pathological subpopulation of osteoclasts, accompanied with suppressed expression of integrin regulating molecule kindling-3 and talin-1.2018
Author(s)
Shiratori T., Kyumoto-Nakamura Y., Kukita A., Uehara N., Zhang J., Koda K., Kamiya M., Badawy T., Tomoda E., Xu X., Yamaza T., Urano Y., Koyano K., Kukita T.
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Journal Title
J. Immunology
Volume: 200
Issue: 4
Pages: 218-228
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] IL-1beta induce pathologically activated osteoclasts bearing extremely high level of resorption activity imaged by pH-sensitive fluorescence probes: A possible pathological subpopulation of osteoclasts, accompanied with suppressed expression of integrin regulating molecule kindling-3 and talin-1.2018
Author(s)
Shiratori T., Kyumoto-Nakamura Y., Kukita A., Uehara N., Zhang J., Koda K., Kamiya M., Badawy T., Tomoda E., Xu X., Yamaza T., Urano Y., Koyano K., Kukita T.
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Journal Title
J. Immunology
Volume: 200
Issue: 1
Pages: 218-228
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Generation mechanism of RANKL+ effector memory B cells: its relevance to the pathogenesis of rheumatoid arthritis2016
Author(s)
Ota Y., Niiro H., Ota S., Ueki N., Tsuzuki H., Nakayama T., Mishima K., Higashioka K., Jabbarzadeh-Tabrizi S., Mitoma H., Akahoshi M., Arinobu Y., Kukita A., Yamada H., Tsukamoto H., Akashi K.
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Journal Title
Arthritis Reseach & Therapy
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.2016
Author(s)
Sonoda S, Yamaza H, Ma L, Tanaka Y, Tomoda E, Aijima R, Nonaka K, Kukita T, Shi S, Nishimura F, Yamaza T.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 19286-19286
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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