Studies for expression and function of Runx2 isoforms

• Project/Area Number: 15K11048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KAWANE Tetsuya 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (00265208)
• Co-Investigator(Kenkyū-buntansha): 小守 壽文 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)
• Co-Investigator(Renkei-kenkyūsha): MIYAZAKI Toshihiro 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10174161) ; MORIISHI Takeshi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (20380983)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Runx2 / isoform / 骨・軟骨 / 骨・軟骨組織 / アイソフォーム / 骨代謝 / 転写因子

Outline of Final Research Achievements

Runx2 is the essential transcription factor for osteoblast differentiation and latter term of chondrocyte differentiation involved in bone formation. Runx2 is expressed as two isoforms (type I and type II) encoded by two different mRNAs with distinct 19 N-terminal amino acids sequence. The type I and type II Runx2 are expressed from the upper stream promoter (P2) and downstream promoter (P1), respectively. In this study, we investigate the regulation of Type I Runx2 expression through prenatal period in mice. As a result, strong expression were detected in osteoblasts and expanded cartilage layer chondrocytes, although little signal was detected in hypertrophic chondrocytes. Therefore, these two isoforms were regulated by different manner.

Research Products

• [Journal Article] Runx2, an inducer of osteoblast and chondrocyte differentiation (2018)
  • Author(s): Komori T
  • Journal Title: Histochem Cell Biol Volume: 印刷中 Issue: 4 Pages: 313-323
  • DOI: 10.1007/s00418-018-1640-6
  • Peer Reviewed / Open Access
• [Journal Article] 骨形成を制御する転写因子Runx2とOsterix (2017)
  • Author(s): 川根徹也，小守壽文
  • Journal Title: 分子リウマチ治療 Volume: 10 Pages: 28-32
• [Journal Article] Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy (2017)
  • Author(s): Yang Mengyu、Arai Atsushi、Udagawa Nobuyuki、Hiraga Toru、Lijuan Zhao、Ito Susumu、Komori Toshihisa、Moriishi Takeshi、Matsuo Koichi、Shimoda Kouji、Zahalka Ali H.、Kobayashi Yasuhiro、Takahashi Naoyuki、Mizoguchi Toshihide
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 4928-4928
  • DOI: 10.1038/s41598-017-05401-1
  • NAID: 120006576162
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Overexpression of Sp7 in odontoblasts results in dentinogenesis imperfecta due to the inhibition of odontoblast maturation (2017)
  • Author(s): Miyazaki T, Inoue M, Baba T, Komori T
  • Journal Title: J Oral Biosci Volume: 59 Pages: 113-120
  • Peer Reviewed
• [Journal Article] Cbfb2 Isoform Dominates More Potent Cbfb1 and Is Required for Skeletal Development. (2016)
  • Author(s): Jiang Q, Qin X, Kawane T, Komori H, Matsuo Y, Taniuchi I, Ito K, Izumi S, Komori T
  • Journal Title: J Bone Miner Res Volume: 印刷中 Issue: 7 Pages: 1391-1404
  • DOI: 10.1002/jbmr.2814
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cbfb regulates bone development by stabilizing Runx family proteins. (2015)
  • Author(s): Qin X, Jiang Q, Matsuo Y, Kawane T, Komori H, Moriishi T, Taniuchi I, Ito K, Kawai Y, Rokutanda S, Izumi S, Komori T.
  • Journal Title: Journal of bone and mineral research Volume: 30 Issue: 4 Pages: 706-714
  • DOI: 10.1002/jbmr.2379
  • Peer Reviewed
• [Presentation] 軟骨細胞におけるGalnt3の過剰発現はムチン型O-グリカンを増加させグリコサミノグリカンを減少させることによってdwarfismを引き起こす (2015)
  • Author(s): 川根徹也, 森石武史, 小守寿人, 小守壽文
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 京王プラザホテル（東京都・新宿区）
  • Year and Date: 2015-07-23