Odontoblast/neuron/endothelial cell signaling network that regulates pulpitis
| Project/Area Number |
15K11056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田崎 雅和 東京歯科大学, 歯学部, 教授 (40155065)
佐藤 正樹 東京歯科大学, 歯学部, 講師 (80598855)
木村 麻記 東京歯科大学, 歯学部, 講師 (90582346)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 歯学 / シグナル伝達 / 神経科学 / 生理学 / 歯髄 / 象牙質 |
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| Outline of Final Research Achievements |
We investigated effects of signaling molecules, that regulate dental pulp inflammation, on cells in the dental pulp and signal transduction network among them. In this research project, we showed that odontoblasts play important role in the sensory transduction for dentinal pain, as sensory receptor cells. When direct mechanical stimulation, modeled as increase of tissue pressure during pulpitis, was applied to single trigeminal ganglion neurons, we could observe sodium-permeable inward current and intracellular free calcium concentration increases. Trigeminal ganglion neurons, thus, have mechanical sensitivity. We could not observe establishment of intercellular communication between them, however. Odontoblasts expressed substance P/neurokinin A/ATP receptors. The results suggested that cells in the dental pulp expressed various receptors for the signaling molecules that regulate dental pulp inflammation. They may mediate a multi signaling network among them intercellularly.
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| Academic Significance and Societal Importance of the Research Achievements |
本申請研究から、歯髄炎制御シグナル分子が、どのように多種細胞で構成される歯髄の細胞情報伝達系に作用するのかについて、その一端が明らかとなった。これらの分子とその受容体は、歯髄炎の治療標的候補になる可能性があり、分子を標的とする先進歯科医療開発の布石となる。本研究の経過中に、象牙芽細胞に作用することで象牙質を形成する分子の発見につながった。将来的な象牙質再生医療への布石ともなった。
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Report
(5 results)
Research Products
(90 results)
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[Journal Article] Transient receptor potential cation channel subfamily V member 3 (TRPV3) is not involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells2015
Author(s)
Higashikawa A, Kojima Y, Sato M, Kimura M, Ogura K, Mochizuki H, Sase T, Shinya A, Kobune K, FuruyaT, Sato T, Shibukawa Y, Tazaki M
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Journal Title
The Bulletin of Tokyo Dental College
Volume: 56
Pages: 259-262
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Depolarization-induced intracellular free calcium concentration increases do not show a desensitizing effect in rat odontoblasts2015
Author(s)
Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
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Journal Title
The Bulletin of Tokyo Dental College
Volume: 56
Pages: 131-134
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Dentin and pulp sense cold stimulus.2015
Author(s)
Tokuda M, Tatsuyama S, Fujisawa M, Morimoto-Yamashita Y, Kawakami Y, Shibukawa Y, Torii M.
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Journal Title
Medical Hypotheses
Volume: 84
Issue: 5
Pages: 442-444
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction2015
Author(s)
Shibukawa, Y., Sato, M., Kimura, M., Sobhan, U., Shimada, M., Nishiyama, A., Kawaguchi, A., Soya, M., Kuroda, H., Katakura, A., Ichinohe, T., and Tazaki, M.
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Journal Title
Pflügers Archiv European Journal of Physiology
Volume: 467
Issue: 4
Pages: 843-863
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Light Conditions Affect Rhythmic Expression of Ano1 in Submandibular Glands2018
Author(s)
Satou, R., Kimura, M., Ishizuka, Y., Suzuki, S., Shibukawa, S., Sugihara, N.
Organizer
96th General Session & Exhibition of the IADR
Related Report
Int'l Joint Research
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[Presentation] Activation of Gs-protein-coupled receptors increases intracellular cAMP level in odontoblasts2018
Author(s)
Kimura, M., Higashikawa, A., Ohyama,S., Mochizuki, H., Kouno, K., Ando, M., Tazaki, M., Shibukawa, Y.
Organizer
96th General Session & Exhibition of the IADR
Related Report
Int'l Joint Research
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[Presentation] Comparison of electrophysiological properties of P2X7 receptors between neurons and glial cells in rat trigeminal ganglion2018
Author(s)
Inoue, H., Kuroda, H., Ishikawa, N., Kimura, M., Shibukawa, Y., Tazaki M., Ichinohe, T.
Organizer
第95回日本生理学会大会
Related Report
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[Presentation] Alkali- and ADP-sensitive store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts2017
Author(s)
Kimura, M., Sato, M., Kojima, Y., Higashikawa, A., Shiozaki, Y., Satou, Y., Shigefuji, R., Shimada, M., Kuroda, H., Ogura, K., Mochizuki, H., Kouno, K., Shibukawa, Y., Tazaki, M.
Organizer
第94回日本生理学会
Related Report
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[Presentation] Odontoblasts express plasma membrane Ca2+-ATPase 1 and 42017
Author(s)
Kimura, M., Higashikawa, A., Kojima, Y., Terashima-Shigefuji, R., Inoue, H., Nagai, S., Kamata, S., Shimada, M., Kuroda, H., Ogura, K., Mochizuki, H., Kouno, K., Ando, M., Shibukawa, Y., Tazaki, M.
Organizer
第95回日本生理学会大会
Related Report
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[Presentation] A voltage-dependent K+ current in rat odontoblasts,2016
Author(s)
Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Shibukawa Y, Tazaki M.
Organizer
The 93rd Annual Meeting of the Physiological Society of Japan
Place of Presentation
Sapporo, Hokkaido, Japan
Year and Date
2016-03-22
Related Report
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[Presentation] Expression of store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts2016
Author(s)
Kimura M, Sato M, Kojima Y, Higashikawa A, Shiozaki Y, Satou R, Shigefuji R, Shimada M, Kobune K, Ogura K, Mochizuki H, Kouno K, Shibukawa Y, Tazaki M
Organizer
The 93rd Annual Meeting of the Physiological Society of Japan
Place of Presentation
Sapporo, Hokkaido, Japan
Year and Date
2016-03-22
Related Report
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[Presentation] Evoked inward currents in trigeminal ganglion neurons caused by intercellular transmitters, ATP, released from mechanically stimulated odontoblast2016
Author(s)
Sato M, Kobune K, Kimura M, Kojima Y, Higashikawa A, Ogura K,Mochizuki H, Kouno K, Shimada M, Shibukawa Y, Tazaki M.
Organizer
The 93rd Annual Meeting of the Physiological Society of Japan
Place of Presentation
Sapporo, Hokkaido, Japan
Year and Date
2016-03-22
Related Report
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[Presentation] Activated mechanisms of H+-sensing ionotropic receptors and G-protein-coupled H+-sensing receptors in odontoblasts2015
Author(s)
Sato M, Kurasawa K, Okabayashi K, Kimura M, Kojima Y, Higashikawa A, Shimada M, Ogura K, Mochizuki H, Sase T, Shinya A, Kobune K, Shibukawa Y and Tazaki M
Organizer
Tripartite Conference on Tooth and Bone; Development & Regeneration
Place of Presentation
Narita, Chiba, Japan
Year and Date
2015-06-12
Related Report
Int'l Joint Research
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[Presentation] Alkaline-sensitive intracellular Ca2+ signaling pathway in rat odontoblasts2015
Author(s)
Kimura M, Sato M, Kojima Y, Higashikawa A, Nishiyama A, Satou R, Shiozaki Y, Shimada M, Ogura K,Mochizuki H, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
Organizer
Tripartite Conference on Tooth and Bone; Development & Regeneration
Place of Presentation
Narita, Chiba, Japan
Year and Date
2015-06-12
Related Report
Int'l Joint Research
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