| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Mouse salivary glands at embryonic day 13 (E13) have been a model organ to study epithelial-mesenchymal interactions. We reported that mesenchymal-derived microRNAs (miRNAs) in the salivary gland are transported to the epithelium. The miRNAs transported from the mesenchyme could target multiple RNAs such as mRNA and long non-coding RNA in the epithelium. Therefore it is hypothesized that those RNAs build an RNA network and influence the expression level of each other by competing for miRNA binding. To explore the RNA network between tissues during salivary gland organogenesis, miRNA transport from the mesenchyme to the epithelium was inhibited and then transcripts in the epithelium was analyzed with RNA-seq. The data showed up-regulation of 27 transcripts expression levels by inhibition of miRNA transport, suggested that these miRNAs and transcripts comprise an RNA network between tissues in E13 mouse salivary glands.
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