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The Mechanism of New Bone Regeneration in New Salmon DNA Scaffold

Research Project

Project/Area Number 15K11062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

KAJIYA HIROSHI  福岡歯科大学, 口腔歯学部, 講師 (80177378)

Co-Investigator(Kenkyū-buntansha) 大野 純  福岡歯科大学, 口腔歯学部, 教授 (10152208)
Research Collaborator IMAMURA AYAKA  福岡歯科大学, 口腔歯学部, 大学院
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨再生 / 骨芽細胞 / DNA / 細胞遊走能 / リン酸輸送体 / スカフォールド / 溶解速度 / リン酸 / バイオマテリアル / リン輸送体
Outline of Final Research Achievements

We previously reported the promotion of bone regeneration in calvarial defects of rats, with DNA/protamine scaffold. However, the method by which this DNA-based scaffold promotes bone formation is still not understood. We examined the effects of salmon DNA on osteoblastic differentiation and calcification in mouse and human preosteoblasts cells in vitro and bone regeneration in a calvarial defect model of in vivo. The DNA was upregulated the expression of the osteogenic markers in preosteoblasts. The DNA scaffold degraded phosphate ions were released to the cell cultures. Furthermore, DNA enhanced migration and upregulated the expression of Na/Pi cotransporters in preosteoblasts. The rodent bone defects implanted with DNA disks promoted new bone formation. Our results indicate that the phosphate released from DNA activated the Na/Pi cotransporters and the migration of osteogenic cells, resulting in the promotion of bone regeneration.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (13 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Rab44, a novel large Rab GTPase, negatively regulates osteoclast differentiation by modulating intracellular calcium levels followed by NFATc1 activation.2018

    • Author(s)
      Yu Yamaguchi, Eiko Sakai, Kuniaki Okamoto, Hiroshi Kajiya, Koji Okabe, Mariko Naito, Tomoko Kadowaki and Tsukuba T
    • Journal Title

      Cell Mol Life Sci.

      Volume: 75 Issue: 1 Pages: 33-48

    • DOI

      10.1007/s00018-017-2607-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micro-computed tomography for evaluating alveolar bone resorption induced by hyperocclusion2018

    • Author(s)
      Tsutsumi T, Kajiya H, Tsuzuki T, Goto KT, Okabe K, Takahashi Y.
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 62 Issue: 3 Pages: 298-302

    • DOI

      10.1016/j.jpor.2017.11.004

    • NAID

      130007458263

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of M1 macrophages in oral mucosal lesions during the development of acute graft-versus-host disease in rats2017

    • Author(s)
      Seno K.、Yasunaga M.、Kajiya H.、Izaki-Hagio K.、Morita H.、Yoneda M.、Hirofuji T.、Ohno J.
    • Journal Title

      Clinical & Experimental Immunology

      Volume: 190 Issue: 3 Pages: 315-327

    • DOI

      10.1111/cei.13043

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The crucial role of the TRPM7 kinase domain in the early stage of amelogenesis2017

    • Author(s)
      Ogata Kayoko、Tsumuraya Tomoyuki、Oka Kyoko、Shin Masashi、Okamoto Fujio、Kajiya Hiroshi、Katagiri Chiaki、Ozaki Masao、Matsushita Masayuki、Okabe Koji
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 18099-18099

    • DOI

      10.1038/s41598-017-18291-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Salmon DNA accelerates bone regeneration by inducing osteoblast migration.2017

    • Author(s)
      Sato A, Kajiya H, Mori N, Sato H, Fukushima T, Kido H, Ohno J*.
    • Journal Title

      PLoS One.

      Volume: 12 Issue: 1 Pages: e0169522-e0169522

    • DOI

      10.1371/journal.pone.0169522

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hyperocclusion stimulates the expression of collagen type XII in periodontal ligament.2016

    • Author(s)
      Tsuzuki T, Kajiya H, T-Goto K, Tsutsumi T, Nemoto T, Okabe K, Takahashi Y.
    • Journal Title

      Arch Oral Biol.

      Volume: 66 Pages: 86-91

    • DOI

      10.1016/j.archoralbio.2016.02.009

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A salmon DNA scaffold promotes osteogenesis through activation of sodium-dependent phosphate cotransporters.2015

    • Author(s)
      Katsumata Y, Kajiya H*, Okabe K, Fukushima T, Ikebe T.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 468 Issue: 4 Pages: 622-628

    • DOI

      10.1016/j.bbrc.2015.10.172

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Photothermal stress triggered by near-infrared-irradiated carbon nanotubes up-regulates osteogenesis and mineral deposition in tooth-extracted sockets.2015

    • Author(s)
      Kajiya H*, Katsumata Y, Sasaki M, Tsutsumi T, Kawaguchi M, Fukushima T.
    • Journal Title

      Int J Hyperthermia.

      Volume: 31 Issue: 6 Pages: 635-642

    • DOI

      10.3109/02656736.2015.1041430

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Photothermal stress triggered by near infrared-irradiated carbon nanotubes promotes bone deposition in rat calvarial defects2015

    • Author(s)
      T.Yanagi, H. kajiya, M. Kawagchi, H. Kido, T. Fukushima
    • Journal Title

      Biomaterials application

      Volume: 29 Issue: 8 Pages: 1109-1118

    • DOI

      10.1177/0885328214556913

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] エナメル質形成におけるTRPM7のキナーゼ活性とチャネル機能の意義2018

    • Author(s)
      進正史, 緒方佳代子, 圓谷智之, 岡暁子, 岡本富士雄, 鍛治屋浩, 片桐千秋, 尾崎正雄, 松下正之, 岡部幸司
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] DFATスフェロイドを応用した骨再生2017

    • Author(s)
      柳束、鍛治屋浩、今村彩香、加倉加恵、城戸寛史、大野純
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(仙台市青葉区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] スフェロイド培養における骨分化誘導へのWnt3aの効果2017

    • Author(s)
      利光拓也、今村彩香、安永まどか、松尾聡、小島寛、鍛治屋浩、大野純
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(仙台市青葉区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 消失速度を調整したサケ白子由来DNAスキャフォールドが骨形成に及ぼす影響2017

    • Author(s)
      松本彩子、森南奈、鍛治屋浩、城戸寛史、大野純
    • Organizer
      第16回日本再生医療学会
    • Place of Presentation
      仙台国際センター(仙台市青葉区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] TRPM7のキナーゼドメインを介したエナメル質形成制御2017

    • Author(s)
      進正史, 緒方佳代子, 岡暁子, 岡本富士雄, 鍛治屋浩, 岡部幸司
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] MMP20過剰発現はエナメル芽細胞の細胞極性障害によりエナメル質形成不全を呈する.2017

    • Author(s)
      進正史, 岡本富士雄, 鍛治屋浩, 緒方佳代子, 岡部幸司
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TRPM7キナーゼを介するリン酸化シグナルとエナメル質形成制御2017

    • Author(s)
      進正史, 緒方佳代子, 圓谷智之, 岡暁子, 岡本富士雄, 鍛治屋浩, 片桐千秋, 尾崎正雄, 松下正之, 岡部幸司
    • Organizer
      第68回西日本生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サケDNAスカフォールドはリン酸輸送体の活性を介して骨形成を促進する2017

    • Author(s)
      勝俣由里, 鍛治屋浩, 首藤俊一, 池崎晶二郎, 田中文恵, 永嶌勝之, 永沼香織, 見立英史, 米津博文, 泉喜和子, 平木昭光、池辺哲郎
    • Organizer
      第71回日本口腔科学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] メバロン酸代謝物による顎骨壊死の 予防・改善効果の可能性2016

    • Author(s)
      鍛治屋浩、大城希美子、岡本富士雄、岡部幸司
    • Organizer
      第23回日本歯科医学会総会
    • Place of Presentation
      福岡国際センター(福岡)
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
  • [Presentation] エナメル質石灰化に関わるTRPチャネルの発現と機能2016

    • Author(s)
      岡部幸司、緒方佳代子、進正史、岡本富士雄、岡暁子、鍛治屋浩
    • Organizer
      第58回歯科基礎医学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 骨伝導能を有する多孔性DNAスキャホールドの開発2016

    • Author(s)
      佐藤絢子、松本彩子、柳束、大野純、鍛治屋浩、城戸寛史、橋本修一
    • Organizer
      第15回日本再生医療学会
    • Place of Presentation
      大阪国際会議場(大阪市北区)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 幹細胞併用のためのサケ白子由来の骨誘導性DNAスカフォールドの開発2015

    • Author(s)
      松本彩子、森南奈、鍛治屋浩、岡部幸司、城戸寛史、福島忠男
    • Organizer
      第37回日本バイオマテリアル学会
    • Place of Presentation
      京都テルサ(京都市南区)
    • Year and Date
      2015-11-09
    • Related Report
      2015 Research-status Report
  • [Presentation] サケ白子由来新規スカフォールドはNa+依存性リン酸輸送体の発現を介し新生骨形成を促進する2015

    • Author(s)
      勝俣由里、鍛治屋浩、大野純、岡部幸司、池邉哲郎
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Book] 「歯科再生・修復医療と材料」 第1編骨組織の再生 第5章サケ由来骨再生材料2015

    • Author(s)
      福島忠男、大野 純、鍛治屋 浩、御手洗 誠、早川 徹
    • Total Pages
      15
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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