Hypoxia-responsive MYC promotes the survival and growth of pleomorphic adenoma cells in hypoxic conditions.
| Project/Area Number |
15K11069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
阿部 達也 新潟大学, 医歯学総合研究科, 特任助教 (70634856)
山崎 学 新潟大学, 医歯学系, 助教 (10547516)
程 クン 新潟大学, 医歯学系, 准教授 (40207460)
朔 敬 大阪歯科大学, 歯学部, 客員教授 (40145264)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 唾液腺多形性腺腫 / 低酸素 / HIF-1α / c-Myc / SM-AP |
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| Outline of Final Research Achievements |
On the basis of hypovasucularity of the salivary pleomorphic adenoma, we had a hypothesis that pleomorphic adenoma cells are able to survive in hypoxic conditions. To understand the hypoxia-dependent manner of energy metabolism in this particular tumor, we analyzed function of MYC, which are the most important factor of metabolic regulation, in cell proliferation and migration in hypoxic-conditioned pleomorphic adenoma cells. In hypoxic condition, SM-AP cells, human pleomorphic adenoma cell systems, showed higher gene expression levels of HIF-1α and MYC in hypoxia. HIF-1α protein was also kept in higher levels and localized more significantly in nuclei. The proliferation and migration of SM-AP cells were reduced under the lack of MYC expression. These results indicated that hypoxic conditions induced pleomorphic adenoma cells to produce MYC, which was maintained by high HIF-1α protein levels.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Aberrant expression of the tight junction molecules claudin-1 and zonula occludens-1 mediates cell growth and invasion in oral squamous cell carcinoma2016
Author(s)
Babkair H, Yamazaki M, Uddin MS, Maruyama S, Abe T, Essa A, Sumita Y, Ahsan MS, Swelam W, Cheng J, Saku T
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Journal Title
Human Pathology
Volume: 57
Pages: 51-60
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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