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Study on elucidation of the pathophysiology of rare intractable diseases (reticular dysgenesis) with severe combined immunodeficiency and development of therapeutic methods

Research Project

Project/Area Number 15K11072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionThe University of Tokushima

Principal Investigator

NOMA Takafumi  徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (40189428)

Co-Investigator(Kenkyū-buntansha) 谷村 綾子  徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (10610199)
堀口 大吾  徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (70304532)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords幹細胞 / ミトコンドリア / エネルギー代謝 / 血球細胞分化制御 / 重症複合型免疫不全症 / 細網異形成症 / AK2 / 遺伝子変異 / 細胞分化 / 組織特異的遺伝子発現 / アデニル酸キナーゼ2 / 制御性T細胞 / FoxP3遺伝子 / テトラサイクリン / 誘導型発現ベクター
Outline of Final Research Achievements

The inducible FOXP3 expression vector was transduced into T cell-derived Jurkat cells by several methodology. After selection with puromycin,47 clones were obtained. Although all clones were analyzed for FoxP3 inducibility, no clone effectively induced FOXP3 gene expression by addition of doxycycline. From the verification experiments, it was suggested that the expression efficiency of the full - length isoform (v1) of FOXP3 gene in Jurkat cells is considerably lower than that of the v2 isoform lacking exon 2, possibly due to the involvement of cell - specific stability.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors2018

    • Author(s)
      Oshima Koichi、Watanabe Akira、Nakahata Tatsutoshi、Saito Megumu K. et al.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 497 Issue: 2 Pages: 719-725

    • DOI

      10.1016/j.bbrc.2018.02.139

    • NAID

      120006466601

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] From Molecular Structure and Mechanisms to Cellular Bioenergetics in Health and Disease2017

    • Author(s)
      Tanimura A, Miyoshi K, Horiguchi T, Hagita H, Fujisawa K, Noma T
    • Organizer
      Gordon Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリアがUPR活性をコントロールして免疫細胞分化の方向を決定づける2017

    • Author(s)
      谷村綾子、三好圭子、堀口大吾、萩田浩子、藤沢浩一、野間隆文
    • Organizer
      第17回日本ミトコンドリア学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/Cas9を用いたAK2の段階的欠損による細胞代謝への影響2017

    • Author(s)
      谷村綾子、三好圭子、堀口大吾、藤沢浩一、野間隆文
    • Organizer
      第2回日本ゲノム編集学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Potential and application of oral mucosa in regenerative medicine2016

    • Author(s)
      野間 隆文
    • Organizer
      NATIONAL COLLOQUIUM ON STEM CELL RESEARCH 2016
    • Place of Presentation
      コタバル市、マレーシア
    • Year and Date
      2016-03-07
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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