| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We clarified the role of ω-3 fatty acids in periodontopathic bacteria-invaded macrophages. Human macrophage cell line THP-1 was invaded with Periodontopathic bacterium. To make knock down cells, siRNA was induced to macrophages by electroporation. Periodontopathic bacteria invasion induced the expression of IL-1β and gasdermin D in THP-1 cells, concurrently with cell death. DHA downregulated cell death and the secretion of IL-1β from periodontopathic bacteria-invaded THP-1 cells. DHA also downregulated the expression of inflammasome-related protein induced by periodontopathic bacteria invasion in THP-1 cells. Depletion of caspase-4 downregulated the expression of IL-1β and gasdermin D in periodontopathic bacteria-invaded THP-1 cells. These results indicate that ω-3 fatty acid, particularly DHA, downregulated the inflammasome activity in THP-1 cells induced by periodontopathic bacteria through the downregulation of ASC and caspase-4 expression.
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