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Molecular biological analysis on periodontal disease and obesity centered on fat metabolism analysis of bacterial invaded macrophages

Research Project

Project/Area Number 15K11083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionKyushu Dental College

Principal Investigator

OKINAGA TOSHINORI  九州歯科大学, 歯学部, 講師 (60582773)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsインフラマソーム / マクロファージ / ピロトーシス / オメガ3系脂肪酸 / サイトカイン / 歯周病細菌 / GasderminD / 炎症性サイトカイン / 脂肪酸 / 不飽和脂肪酸
Outline of Final Research Achievements

We clarified the role of ω-3 fatty acids in periodontopathic bacteria-invaded macrophages. Human macrophage cell line THP-1 was invaded with Periodontopathic bacterium. To make knock down cells, siRNA was induced to macrophages by electroporation. Periodontopathic bacteria invasion induced the expression of IL-1β and gasdermin D in THP-1 cells, concurrently with cell death. DHA downregulated cell death and the secretion of IL-1β from periodontopathic bacteria-invaded THP-1 cells. DHA also downregulated the expression of inflammasome-related protein induced by periodontopathic bacteria invasion in THP-1 cells. Depletion of caspase-4 downregulated the expression of IL-1β and gasdermin D in periodontopathic bacteria-invaded THP-1 cells. These results indicate that ω-3 fatty acid, particularly DHA, downregulated the inflammasome activity in THP-1 cells induced by periodontopathic bacteria through the downregulation of ASC and caspase-4 expression.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 3 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Ameloblastin Upregulates Inflammatory Response through Induction of IL-1β in Human Macrophages2017

    • Author(s)
      Otsuka M, Okinaga T, Ariyoshi W, Kitamura C, Nishihara T.
    • Journal Title

      J Cell Biochem

      Volume: - Issue: 10 Pages: 3308-3317

    • DOI

      10.1002/jcb.25983

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High molecular weight hyaluronic acid regulates MMP13 expression in chondrocytes via DUSP10/MKP52017

    • Author(s)
      Furuta J, Ariyoshi W, Okinaga T, Takeuchi J, Mitsugi S, Tominaga K, Nishihara T.
    • Journal Title

      J Orthop Res

      Volume: 35 Issue: 12 Pages: 331-339

    • DOI

      10.1002/jcp.25809

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanisms involved in enhancement of matrix metalloproteinase-9 expression in macrophages by interleukin-332017

    • Author(s)
      Ariyoshi W, Okinaga T, Chaweewannakorn W, Akifusa S, Nisihara T
    • Journal Title

      J Cell Physiol

      Volume: 20

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ameloblastin and enamelin prevent osteoclast formation by suppressing RANKL expression via MAPK signaling pathway2017

    • Author(s)
      Chaweewannakorn W, Ariyoshi W, Okinaga T, Morikawa K, Saeki K, Maki K, Nishihara T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 485 Issue: 3 Pages: 621-626

    • DOI

      10.1016/j.bbrc.2017.01.181

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Essential role of mitogen-activated protein kinases in IL-17A-induced MMP-3 expression in human synovial sarcoma cells2016

    • Author(s)
      Sakurai T, Yoshiga D, Ariyoshi W, Okinaga T, Kiyomiya H, Furuta J, Yoshioka I, Tominaga K, Nishihara T
    • Journal Title

      BMC Res Notes

      Volume: 9 (1) Issue: 1 Pages: 68-68

    • DOI

      10.1186/s13104-016-1892-y

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diagnosis of Periodontal Disease from Saliva Samples Using Fourier Transform Infrared Microscopy Coupled with Partial Least Squares Discriminant Analysis2016

    • Author(s)
      Fujii S, Sato S, Fukuda K, Okinaga T, Ariyoshi W, Usui M, Nakashima K, Nishihara T, Takenaka S.
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 2 Pages: 225-231

    • DOI

      10.2116/analsci.32.225

    • NAID

      130005125648

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lysophosphatidylethanolamina acyltransfcrase/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons.2016

    • Author(s)
      Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H,Okinaga T, Shimizu T, Tominaga K,Nishihara T.
    • Journal Title

      FASEB. J

      Volume: 30 Issue: 7 Pages: 2591-2601

    • DOI

      10.1096/fj.201500097r

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp2016

    • Author(s)
      Jaffar N, Ishikawa Y, Mizuno K, Okinaga T, Maeda T
    • Journal Title

      PLos one

      Volume: 11 Issue: 7 Pages: e0159466-e0159466

    • DOI

      10.1371/journal.pone.0159466

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Essential Role of Lysophosphatidylcholine Acyltransferase 3 in the Induction of Macrophage Polarization in PMA-Treated U937 Cells2015

    • Author(s)
      Taniguchi K, Hikiji H, Okinaga T, Hashidate-Yoshida T, Shindou H, Ariyoshi W, Shimizu T, Tominaga K, Nishihara T
    • Journal Title

      J Cell Biochem

      Volume: 116 Issue: 12 Pages: 2840-2848

    • DOI

      10.1002/jcb.25230

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The role of Docosahexaenoic acid on inflammasome activity induced by A. actinomycetemcomitans invasion macrophages2017

    • Author(s)
      沖永敏則、有吉渉、西原達次
    • Organizer
      日本細菌学会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] オメガ3脂肪酸は歯周病細菌が誘導するインフラマソーム活性を抑制する2017

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ドコサヘキサエン酸は歯周病細菌誘導インフラマソーム活性を制御する2017

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      第70回日本細菌学会九州支部総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Omega-3 fatty acids regulate inflammasome activity and pyroptosis induced by A. actinomycetemcomitans invasion in macrophages2017

    • Author(s)
      Okinaga T, Ariyoshi W, Nishihara T
    • Organizer
      15th International Congress of Bacteriology and Applied Microbiology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of Docosahexaenoic acid on inflammasome activity induced by A. actinomycetemcomitans invasion macrophages2017

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      第90回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A. actinomycetemcomitansが誘導するサイトカイン産生に対するオメガ3脂肪酸の影響2016

    • Author(s)
      沖永敏則、有吉渉、西原達次
    • Organizer
      日本細菌学会九州支部総会
    • Place of Presentation
      宮崎
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] クロファージ分化におけるω-3系脂肪酸の役割2016

    • Author(s)
      川野亜希、沖永敏則、引地尚子、有吉渉、西原達次
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      北海道
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] オメガ3脂肪酸は歯周病細菌侵入マクロファージが誘導するインフラマソーム活性を抑制する2016

    • Author(s)
      沖永敏則、有吉渉、西原達次
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      北海道
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Docosahexaenoic acid downregulates inflammasome activity and endocytosis in A. actinomycetemcomitans-invaded macrophages2016

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      日本細菌学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] オメガ3脂肪酸は歯周病細菌が誘導するインフラマソーム活性を抑制する2015

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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