Analysis of the BMAL1 knockout mice using in vivo micro-CT

• Project/Area Number: 15K11088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nihon University
• Principal Investigator: HONDA Kazuya 日本大学, 歯学部, 教授 (30199567)
• Co-Investigator(Kenkyū-buntansha): 川嶋 祥史 日本大学, 歯学部, 講師 (30349964) ; 松本 邦史 鹿児島大学, 医歯学域附属病院, 講師 (00508658) ; 江島 堅一郎 日本大学, 歯学部, 講師 (50267093) ; 澤田 久仁彦 日本大学, 歯学部, 講師 (00287658)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: Bmal-1遺伝子ノックアウトマウス / 骨リモデリング， / マイクロCT / 顎関節症 / 変形性関節症 / 骨リモデリング / 顎関節 / 変形性顎関節症 / 異所性石灰化

Outline of Final Research Achievements

Brain and muscle BMAL1 knockout mice exhibit accelerated ageing, glucose metabolism disorder, and adipocyte differentiation, among other phenotypes, as clearly identified effects of the BMAL1 gene. However, studies on the continuous changes in deformation of the mandibular condyle have not been reported. Images of the mandibular condyle were taken after birth under inhalation anesthesia.
In BMAL1 knockout mice, deformation of the mandibular condyle was confirmed in 8 of 12 joints. In wild-type mice, deformation was confirmed in 2 of 12 joints. In BMAL1 knockout mice, deformation in areas surrounding the mandibular condyle, which were considered to undergo calcification, was confirmed in 2 of 12 joints. In wild-type mice, deformation was confirmed in 0 joint. In BMAL1 knockout mice, an ageing phenomenon was observed in the mandibular condyle, indicating that the circadian rhythm affects changes in the shape of the mandibular condyle.

Research Products

• [Journal Article] Observation of changes in shape of the mandibular condyle over time in BMAL1 knockout mice using in vivo micro-CT (2018)
  • Author(s): Shigenori Hirai, Yusuke Hayashi, Motohiro Ito, Toshihiko Amemiya Ko Dezawa, Yoshinori Arai, Ken-ichiro Ejima, Shigeki Shimba, Kazuya Honda
  • Journal Title: Journal of Oral Science Volume: -
  • Peer Reviewed
• [Presentation] in vivo micro CTを用いたBmal-1遺伝子ノックアウトマウスの下顎頭形態変化の継時的観察 (2017)
  • Author(s): 平井重徳，川嶋祥史，林 悠介，出澤 幸，雨宮俊彦，澤田久仁彦，江島堅一郎，新井嘉則，本田和也
  • Organizer: 第58回日本歯科放射線学会総会
• [Presentation] Reduction of mastication activity induces hypotrophy of the mandibular condyle in an OVX animal model: Microcomputed tomography analysis in an experimental animal model (2016)
  • Author(s): Kunihito Matsumoto, Toshihiro Suzuki, Yoshinori Arai, Kazuya Honda
  • Organizer: The 11th ACOMFR Asian Congress of Oral and MaxilloFacial Radiology
  • Place of Presentation: Chiangmai（ Thailand）
  • Year and Date: 2016-11-10
  • Int'l Joint Research
• [Presentation] Reduction of mastication activity induces hypotrophy of the mandibular condyle: Microcomputed tomography analysis in an experimental animal model (2016)
  • Author(s): Kunihito Matsumoto, Toshihiro Suzuki, Yoshinori Arai, Kazuya Honda
  • Organizer: The 15th European Congress of DentoMaxilloFacial Radiology
  • Place of Presentation: Wales（United Kingdom)
  • Year and Date: 2016-06-15
  • Int'l Joint Research