Role of microenvironment on maintenance of odontoblasts: analysis by using novel odontoblast-isolation method
| Project/Area Number |
15K11120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
SHIBATA Yasuaki 長崎大学, 医歯薬学総合研究科(医学系), 講師 (80253673)
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| Co-Investigator(Kenkyū-buntansha) |
池田 通 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00211029)
中島 和慶 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (40707246)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 象牙芽細胞 / 培養 / Tie2 / Angiopoietin-1 / 微小環境 / エナメル器 / 細胞単離 |
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| Outline of Final Research Achievements |
The purpose of this study is to explore the role of microenvironment in maintenance of odontoblast differentiation. We isolated the odontoblast fraction by using Tie2 and CD31 antibody. Expression of differentiation markers in monoculture of the odontoblasts decreased within 1 week, whereas those were maintained when the odontoblasts was co-cultured with the mesenchymal fraction (Tie2-). These results revealed that the microenvironment of the dental pulp is essential for the maintenance of the odontoblast differentiation. In parallel with the study, we explored the expression of Angiopoietin-1 (Ang-1) in mouse enamel organ. Ang-1 was expressed in enamel organ in associated with the invasion of Tie2-positive capillaries at embryonic day 17. Our result suggested that Ang-1/Tie2 system regulates not only odontoblast differentiation but also involution of enamel organ.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Regulation and Biological Significance of Formation of Osteoclasts and Foreign Body Giant Cells in an Extraskeletal Implantation Model2016
Author(s)
Ahmed GJ, Tatsukawa E, Morishita K, Shibata Y, Suehiro F, Kamitakahara M, Yokoi T, Koji T, Umeda M, Nishimura M, Ikeda T
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Journal Title
ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume: 49
Issue: 3
Pages: 97-107
DOI
NAID
ISSN
0044-5991, 1347-5800
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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