| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The purpose of this study is to explore the role of microenvironment in maintenance of odontoblast differentiation. We isolated the odontoblast fraction by using Tie2 and CD31 antibody. Expression of differentiation markers in monoculture of the odontoblasts decreased within 1 week, whereas those were maintained when the odontoblasts was co-cultured with the mesenchymal fraction (Tie2-). These results revealed that the microenvironment of the dental pulp is essential for the maintenance of the odontoblast differentiation. In parallel with the study, we explored the expression of Angiopoietin-1 (Ang-1) in mouse enamel organ. Ang-1 was expressed in enamel organ in associated with the invasion of Tie2-positive capillaries at embryonic day 17. Our result suggested that Ang-1/Tie2 system regulates not only odontoblast differentiation but also involution of enamel organ.
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