| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The purpose of this study is to clarify the mechanism of COX-2 production via PAR-1 activation by kallikrein in human dental pulp cultured cells, and the interaction network of kallikrein and plasmin and bradykinin.
Kallikrein increased the expression of COX-2 mRNA in a concentration- and time-dependent manner. Kallikrein promoted the production of COX-2 protein, and this effect was suppressed by PAR-1 inhibitor.
In the search for the interaction by plasmin · kallikrein · bradykinin, the effect was not confirmed by intracellular Ca2+ concentration measurement. However, the stimulation of kallikrein and bradykinin enhanced the expression of COX-2 protein compared to when stimulated alone.
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