Synergistic network on COX-2 production by PAR-1 activation cascade in pulp
| Project/Area Number |
15K11131
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
KAMIO Naoto 日本大学, 松戸歯学部, 助教 (10508774)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | ヒト歯髄細胞 / カリクレイン / PAR-1 / COX-2 / 炎症 / ヒト歯髄培養細胞 |
|---|
| Outline of Final Research Achievements |
The purpose of this study is to clarify the mechanism of COX-2 production via PAR-1 activation by kallikrein in human dental pulp cultured cells, and the interaction network of kallikrein and plasmin and bradykinin.
Kallikrein increased the expression of COX-2 mRNA in a concentration- and time-dependent manner. Kallikrein promoted the production of COX-2 protein, and this effect was suppressed by PAR-1 inhibitor.
In the search for the interaction by plasmin · kallikrein · bradykinin, the effect was not confirmed by intracellular Ca2+ concentration measurement. However, the stimulation of kallikrein and bradykinin enhanced the expression of COX-2 protein compared to when stimulated alone.
|
Report
(4 results)
Research Products
(4 results)
