Analysis of migration factor expressed in dendritic cells during metal allergy development

• Project/Area Number: 15K11161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: The University of Tokushima
• Principal Investigator: WATANABE Megumi 徳島大学, 病院, 講師 (40380050)
• Co-Investigator(Kenkyū-buntansha): 後藤 崇晴 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (00581381)
• Research Collaborator: ISHIMARU Naozumi ; WL HAVRAN ; UG KNAUS
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯科用金属アレルギー / 樹状細胞 / 上皮角化細胞 / 細胞遊走 / 金属アレルギー / ケラチノサイト / TSLP/TSLPR

Outline of Final Research Achievements

The aim of this study is to clarify the interactions between dendritic cells and keratinocytes during the development of metal allergy. We focused on the interactions of TSLP in keratinocytes and TSLPR in dendritic cells. Expressions of both TSLP and TSLPR in cells stimulated with nickel were increased subsequently. Significant decrease of ear swellings was observed in mice which were injected with TSLPR siRNA in ear skin before the injection of nickel. Therefore, interactions between TSLP in keratinocytes and TSLPR in dendritic cells may play an important role in development of metal allergy and these interactions have a possibility of using for diagnosis or treatment for metal allergy.

Research Products

• [Presentation] 義歯材料とアレルギー (2018)
  • Author(s): 渡邉恵
  • Organizer: 日本義歯ケア学会 第10回学術大会
  • Invited
• [Presentation] ニッケルで刺激したケラチノサイトと樹状細胞におけるPlexin-Semaphorinの動態解析 (2017)
  • Author(s): 南憲一，渡邉 恵，市川哲雄
  • Organizer: （公社）日本補綴歯科学会 第126回学術大会
• [Presentation] 基礎研究からみた金属アレルギー (2016)
  • Author(s): 渡邉 恵
  • Organizer: 第8回 一般社団法人 日本メタルフリー歯科学会学術大会
  • Place of Presentation: 愛知学院大学（愛知県名古屋市）
  • Invited
• [Presentation] 金属アレルギーの発症と免疫 (2015)
  • Author(s): 渡邉恵
  • Organizer: （公社）日本補綴歯科学会 関西支部総会・学術大会
  • Place of Presentation: 兵庫医療大学（兵庫県神戸市）
  • Year and Date: 2015-11-08
  • Invited
• [Presentation] 金属アレルギーの病態 (2015)
  • Author(s): 渡邉恵
  • Organizer: （公社）日本補綴歯科学会第124回学術大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-05-30