| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In the present study, we investigate the relationship between the reduction of bone regeneration and angiogenic potential in the senescence-associated mesenchymal stem cells (MSCs). 20 times passaged aged MSCs (P20) were decreased the proliferation and osteogenic potential than the 3 times passaged young MSCs (P3). Furthermore, angiogenic cytokine expression levels were also decreased in aged MSCs. We next compared the angiogenic effect between the aged HUVECs (P20) and young HUVECs (P3). Aged HUVECs were significantly decreased the angiogenic potential. We next collected the bone marrow MSCs and adipose derived stem cells (ADSCs) from aged mice (20 months old) and young mice (10 weeks old). In aged MSCs and ADSCs the proliferation and osteogenic potential was decreased. Angiogenic cytokine expression levels were also declined. The senescence-associated reduction of bone regeneration may be induced by both MSCs functional disorder and reduction of angiogenic potential.
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