| Project/Area Number |
15K11186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
篠崎 陽介 福岡歯科大学, 口腔歯学部, 助教 (80736687)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | コラーゲン / 酸化ストレス / 骨芽細胞 / コラーゲン架橋形成 / コラーゲン線維形成 / 骨粗鬆症 |
|---|
| Outline of Final Research Achievements |
Oxidative stress induced by hydrogen peroxide repressed the differentiation ofand mineralization by osteoblasts. The oxidative stress altered the bone matrix stained with picrosirius red, with a color change from orange to yellow observed under polarized light microscope, suggesting hindrance of collagen fibril formation. The stress altered gene expression of some of posttranslational modifiers of collagen, which control collagen cross-linking and fiber formation. Although there were no expression changes of collagen fibrillogenesis modulators such as lysyl hydroxylase 1 (LH1), LH2, LH3, and glycosyltransferase family 25 domain 1 (GLT25D1), the expression of some collagen cross-link initiators such as lysyl oxidase (LOX) and lysyl oxidase-like protein 1 (LOXL-1) enhanced. The oxidative stress deteriorates bone matrix with thinning of collagen fibrils, possibly due to the alteration of expression of collagen cross-link initiators, LOX and LOXL-1.
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