Development for primate periodontal tissue regenerative therapy by human iPS cells for xeno-free system
| Project/Area Number |
15K11221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森川 暁 慶應義塾大学, 医学部(信濃町), 講師 (00424169)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯周組織再生 / 神経堤細胞 / 間葉系幹細胞 / iPS細胞 / フィーダーフリー / ゼノフリー |
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| Outline of Final Research Achievements |
In this study, we realized the induction of neural crest cells, which was feeder-free, xeno-free iPS culture method. It was confirmed that neural crest cells existed in the periodontal ligament tissue of the mouse and some of them expressed PDGFRα and CD44 which are mesenchymal stem cell markers. This suggested that neural crest derived mesenchymal stem cells may be a potential source of cells for periodontal tissue regeneration therapy. In order to transplant these cells into real periodontal disease model mice, the disease model mice were prepared and evaluated. Periodontal disease induction model induced bone destruction by ligating 5-0 silk thread to mouse molar tooth. To confirm the technical reproducibility. Now, we have been preparing for transplantation.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration.2015
Author(s)
Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y.
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Journal Title
journal of Dental Research
Volume: 95
Issue: 2
Pages: 206-214
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] LNGFR(+)THY-1(+) multipotent stem cells derived from human induced pluripotent stem cells2017
Author(s)
Ouchi, T. Shibata, S. Kimura, H. Nagoshi, N. Fujimura, Y. Morikawa, S. Sato, K. Kawana, H. Fukuda, K. Nakamura, M. Nakagawa, T. Okano, H.
Organizer
18th International Congress of Developmental Biology
Related Report
Int'l Joint Research
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