| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Transforming growth factor-beta (TGF-β), which is an important cytokine that controls cell proliferation and differentiation, was activated in hPDL cells by Er:YAG laser irradiation. However, the direct activation was not done, and MMP-2, MMP-3, MMP-8 gene expression was involved in its activation, suggesting the involvement of HSP90α.
Er:YAG laser irradiation on the porcine pulp tissue had an effect of increasing ALP activity, and MMPs activity of 53,45,40 kDa and non-MMP activity of 33 kDa were observed. Enhancement of MMP-2 gene expression was recognized by laser irradiation on immortalized cells (PPU-7 cells) derived from porcine pulp tissue, and it was considered to be involved in TGF-β activity.
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