Analysis of damage-associated molecular patterns (DAMPs) in autologous tissue-engineered cartilage and its application to regenerative medicine

• Project/Area Number: 15K11236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: Fujihara Yuko 東京大学, 医学部附属病院, 助教 (50466744)
• Co-Investigator(Kenkyū-buntansha): 星 和人 東京大学, 医学部附属病院, 教授 (30344451)
• Co-Investigator(Renkei-kenkyūsha): TAKATO Tsuyoshi 東京大学, 医学部附属病院, 登録診療員 (90171454)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 軟骨再生医療 / DAMPs

Outline of Final Research Achievements

To obtain stable outcomes in regenerative medicine, quality of cells for transplantation is of great importance. Potentially, cellular stress results in the release of damage-associated molecular patterns (DAMPs), and activates immunological responses, affecting the outcomes of transplanted tissues. In this study, we intentionally prepared necrotic chondrocytes to be gradually dying and releasing DAMPs, and investigated how the viability of chondrocytes could affect the maturation of tissue-engineered cartilage. It was suggested that DAMPs from necrotic chondrocytes could prompt migration of more immune cells, worsening the maturation of tissue-engineered cartilage.

Research Products

• [Journal Article] Roles of macrophage migration inhibitory factor in cartilage tissue engineering. (2018)
  • Author(s): Fujihara Y, Hikita A, Takato T, Hoshi K.
  • Journal Title: J Cell Physiol. Volume: 233(2) Issue: 2 Pages: 1490-1499
  • DOI: 10.1002/jcp.26036
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] T2 and apparent diffusion coefficient of MRI reflect maturation of tissue-engineered auricular cartilage subcutaneously transplanted in rats (2016)
  • Author(s): Fujihara Y, NItta N, Misawa M, Hyodo K, Shirasaki Y, Hayashi K, Kosaka R, Homma K, Numano T, Kuribayashi S, Watanabe Y, Sato J, Ohtomo K, Takato T, Hoshi K
  • Journal Title: Tissue Eng Part C Methods Volume: 22(5) Issue: 5 Pages: 429-438
  • DOI: 10.1089/ten.tec.2015.0291
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Roles of macrophage migration inhibitory factor in cartilage tissue engineering. (2017)
  • Author(s): Fujihara Y, Hikita A, Hoshi K.
  • Organizer: 13th World Congress on Inflammation
  • Int'l Joint Research
• [Presentation] Roles of macrophages in transplantation of tissue engineered cartilage in mice. (2016)
  • Author(s): Fujihara Y, Hikita A, Takato T, Hoshi K.
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne Convention and Exhibition Centre
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] 歯科分野における再生医療 (2015)
  • Author(s): 藤原夕子
  • Organizer: ニューセラミックス懇話会 第２１９回研究会
  • Place of Presentation: 株式会社島津製作所 関西支社 マルチホール（大阪府・大阪市）
  • Year and Date: 2015-10-23
  • Invited
• [Presentation] Basic and clinical research on custom-made artificial bone and tissue-engineered cartilage in oral and maxillofacial region (2015)
  • Author(s): Fujihara Y, Hikita A, Saijo H, Kanno Y, Takato T, Hoshi K
  • Organizer: 2015 4th TERMIS World Congress
  • Place of Presentation: Boston, MA, USA, Boston Marriott Copley Place
  • Year and Date: 2015-09-08
  • Int'l Joint Research