Analysis of the cleft lip/palate development mechanism using the p53 knockout mouse with Artificial insemination frozen embryos

• Project/Area Number: 15K11237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: Kodama Yasumitsu 新潟大学, 医歯学総合病院, 講師 (90419276)
• Co-Investigator(Kenkyū-buntansha): 大峡 淳 新潟大学, 医歯学系, 教授 (40266169)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口蓋裂 / p53遺伝子 / コンソミックマウス / 感受性遺伝子 / 放射線照射 / ノックアウトマウス / 疾患感受性遺伝子

Outline of Final Research Achievements

We regarded p53 gene known as tumor suppressor gene as "a guardian of the teratogenesis".　It was intended to search effect of p53 on cleft lip and palate formation in the CL/Fr mouse (cleft lip and palate sensitive strain).　We analyzed to use the p53 knockout CL/Fr mouse artificial insemination frozen embryos.
Cleft lip in the CL/Frp53(-/-) mouse was suggested to be caused by specific malformation without the report so far.　The cleft palate in the p53(-/-) mouse was almost the clinical condition of so-called complete cleft palate, and the palatine process was found.　The size of the palatine process was very small, and most of the growth to a horizontal direction was proved not to occur.

Research Products

• [Presentation] The role of miRNA in orofacial development (2016)
  • Author(s): Nagai T, Kawasaki M, Kawasaki K, Watanabe M, Nishikawa A, Kodama Y, Takagi R, Maeda T, Ohazama A
  • Organizer: Collaborative symposium. Faculity of Dentistry: Universitas Indonesia-Niigata University
  • Place of Presentation: Lombok、（Indonesia）
  • Year and Date: 2016-01-09
  • Int'l Joint Research
• [Presentation] The role of Ofd1 in palatal development (2016)
  • Author(s): Watanabe M, Kawasaki M, Kawasaki K, Nagai T, Nishikawa A, Kodama Y, Takagi R, Maeda T, Ohazama A
  • Organizer: Collaborative symposium. Faculity of Dentistry: Universitas Indonesia-Niigata University
  • Place of Presentation: Lombok、（Indonesia）
  • Year and Date: 2016-01-09
  • Int'l Joint Research
• [Presentation] 口蓋発生におけるOfd1の役割について (2015)
  • Author(s): 渡部桃子, 川崎真依子, 高木律男, 前田健康, 大峡 淳
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市
  • Year and Date: 2015-09-11