Pathological study for the osteoporosis produced by the osteoblastic niche and hematopoietic stem cells

• Project/Area Number: 15K11246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMACHIKA EIKI 岡山大学, 大学病院, 講師 (10294422)
• Co-Investigator(Kenkyū-buntansha): 辻極 秀次 岡山理科大学, 理学部, 教授 (70335628)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨 / 顎骨壊死 / 骨粗鬆症 / ビスフォスフォネート / 薬剤関連顎骨壊死 / 骨芽細胞 / 破骨細胞 / リンパ球 / 造血幹細胞

Outline of Final Research Achievements

The distribution of T cells was influenced by the ovariectomy and admistrations of bisphosphonate or teriparatide. The number of CD3+ cells within the femur bone marrow of the mice was down-regulated by the ovariectomy and administration of zoledronate. However, this number was up-regulated by the administration of teriparatide.
Mineral/matrix ration was significantly increased by the administration of zoledronate within the newly formed bone in the mandible of mice, however crystallinity, relative proteoglycan content and collgen structural integrity were significantly decreased.

Research Products

• [Journal Article] Molecular alterations of newly formed mandibular bone caused by zoledronate. (2018)
  • Author(s): Yoshioka Y, Yamachika E, Nakanishi M, Ninomiya T, Nakatsuji K, Matsubara M, Moritani N, Kobayashi Y, Fujii T, Iida S
  • Journal Title: Int J Oral Maxillofac Surg Volume: － Issue: 9 Pages: 1206-1213
  • DOI: 10.1016/j.ijom.2018.02.002
  • Peer Reviewed
• [Journal Article] The influence of zoledronate and teriparatide on gamma delta T cells in mice (2017)
  • Author(s): Yamachika Eiki、Matsui Yuichi、Matsubara Masakazu、Matsumura Tatsushi、Nakata Naoki、Moritani Norifumi、Ikeda Atsushi、Tsujigiwa Hidetsugu、Ohara Naoya、Iida Seiji
  • Journal Title: Journal of Dental Science Volume: 12 Issue: 4 Pages: 333-339
  • DOI: 10.1016/j.jds.2017.03.007
  • Peer Reviewed / Open Access
• [Journal Article] 骨吸収抑制薬関連顎骨壊死に由来した非クロストリジウム性ガス壊疽の1例 (2017)
  • Author(s): 松原 正和, 石田 展久, 池田 篤司, 山近 英樹, 明石 翔, 飯田 征二
  • Journal Title: 日本口腔外科学会雑誌 Volume: 63(10) Pages: 506-511
  • NAID: 130006258028
  • Peer Reviewed
• [Journal Article] The influence of zoledronate and teriparatide on gamma delta T cells in mice (2017)
  • Author(s): Eiki Yamachika, Yuichi Matsui, Masakazu Matsubara, Tatsushi Matsumura, Naoki Nakata, Norifumi Moritani, Atsushi Ikeda, Hidetsugu Tsujigiwa, Naoya Ohara, Seiji Iida
  • Journal Title: Journal of Dental Sciences Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Anti-osteoporosis effects of 1,4-dihydroxy-2-naphthoic acid in ovariectomized mice with increasing of bone density (2016)
  • Author(s): Kenichiro Kita, Eiki Yamachika, Masakazu Matsubara, Hidetsugu Tsujigiwa, Nobuhisa Ishida, Norifumi Moritani, Tatsushi Matsumura, Masahide Mizutani, Yuki Fujita, Kiyofumi Takabatake, Kenichiro Ejima, Hitoshi Nagatsuka, Yoshinori Yamaguchi, Seiji Iida
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 28(1) Issue: 1 Pages: 66-72
  • DOI: 10.1016/j.ajoms.2015.07.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Treatment of Osteonecrosis of the Jaw. (2015)
  • Author(s): Yamachika E, Matsubara M, Ikeda A, Matsumura T, Moritani N, Iida S.
  • Journal Title: Journal of Craniofacial Surgery Volume: 27(7) Issue: 7 Pages: 575-577
  • DOI: 10.1097/scs.0000000000002127
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] ゾレドロネート投与による下顎新生骨における分子改変 (2017)
  • Author(s): 吉岡洋祐、山近英樹、中辻和樹、松原正和、森谷徳文、飯田征二
  • Organizer: 第62回（公社）日本口腔外科学会総会・学術大会
• [Presentation] ビスフォスフォネート製剤による免疫細胞への影響についてγδT細胞を指標として (2017)
  • Author(s): 中辻和樹、山近英樹、松井裕一、松原正和、森谷徳文、吉岡洋祐、飯田征二
  • Organizer: 第62回（公社）日本口腔外科学会総会・学術大会
• [Presentation] The Influence of Zoledronate and Teriparatide on ｇｄ T Cells in Mice (2016)
  • Author(s): Eiki Yamachika, Yuichi Matsui, Atsushi Ikeda, Yohsuke Yoshioka, Masakazu Matsubara, Norifumi Moritani, Tatsushi Matsumura, Seiji Iida
  • Organizer: American association of Oral and maxillofacial surgery 98th annual meeting
  • Place of Presentation: Las Vegas, NV
  • Year and Date: 2016-09-21
  • Int'l Joint Research
• [Presentation] 口腔粘膜欠損・歯槽骨欠損に対する自己血由来フィブリンポリマーの有用性 (2015)
  • Author(s): 松原正和，石田展久，吉岡洋祐，山近英樹，仲田直樹，池田篤司，田畑光康，飯田征二
  • Organizer: 第60回日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-16
• [Presentation] テリパラチドとアモキシシリン投与が著効したMRONJ Stage3の1例 (2015)
  • Author(s): 山近英樹，松原正和，松井裕一，森谷徳文，松村達志，吉岡洋祐，田村庄平，妹尾昌紀，有村友紀，飯田征二
  • Organizer: 第60回日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-16
• [Presentation] ビスフォスフォネート系製剤およびテリパラチドがリンパ球分画へ与える影響 (2015)
  • Author(s): 松井裕一，山近英樹，吉岡洋祐，植村亜由美，田村庄平，岸本宣子，山本祐也，松原正和，池田篤司，飯田征二
  • Organizer: 第60回日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-16
• [Presentation] ビスフォスフォネート内服後短期間で発症した顎骨壊死を伴う敗血症で死亡した１例 (2015)
  • Author(s): 佐藤亜希、山近英樹、平田泰久、岡崎文彦、澤木康一、中野誠、飯田征二
  • Organizer: 第60回日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-16
• [Presentation] リゼドロネートおよびテリパラチドがリンパ球分画へ与える影響 (2015)
  • Author(s): 松井 裕一, 山近 英樹, 有村 友紀, 合田 裕, 高畠 清文, 松原 正和, 飯田 征二
  • Organizer: 第69回日本口腔科学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-05-13