Differential analysis and application at the molecular level of the immunosuppressant action base seen from cartilage tissue

• Project/Area Number: 15K11248
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: NAKATA naoki 岡山大学, 医歯薬学総合研究科, 助教 (90626251)
• Co-Investigator(Kenkyū-buntansha): 山近 英樹 岡山大学, 大学病院, 講師 (10294422) ; 滝川 正春 岡山大学, 医歯薬学総合研究科, 教授 (20112063) ; 森谷 徳文 岡山大学, 医歯薬学総合研究科, 助教 (60467751) ; 久保田 聡 岡山大学, 医歯薬学総合研究科, 教授 (90221936)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 免疫抑制剤 / ステロイド

Outline of Final Research Achievements

1) We tried to clarify the regulatory mechanism of CCN2 / CTGF gene expression of tacrolimus in vitro using molecular biological methods, but we could not obtain the expected results.
2) When stimulating HCS-2/8 cells with tacrolimus, analyze exhaustively as well as other variable genes such as dexamethasone, using the microarray method at the time and concentration where CCN2 / CTGF gene expression regulation is the most active Although it was scheduled to do, it could not be achieved due to delay in planning progress.