Analysis of EMT-inducing mechanism in oral cancer

• Project/Area Number: 15K11250
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Hiroshi Shimasue 広島大学, 病院(歯), 助教 (40335683)
• Co-Investigator(Kenkyū-buntansha): 飛梅 圭 広島大学, 医歯薬保健学研究科(歯), 准教授 (40350037) ; 東川 晃一郎 広島大学, 病院(歯), 講師 (80363084) ; 重石 英生 広島大学, 医歯薬保健学研究科(歯), 講師 (90397943)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔癌 / EMT / 浸潤・転移 / Snail / 癌の浸潤

Outline of Final Research Achievements

EMT definately is involved in cancer invasion and metastasis, indeed cancer cells undergo malignant progress with EMT intensity. Snail controls the differentiation transition of oral cancer cells. Abundant expression of Slug, a family molecule of Snail, in basal cells of squamous epithelium contlols generationg the epithelium structure, thus oral squamous basal cells-derived cell line RT-7 showed expression of Slug and aquired cell motility like EMT phenomenon. In oral cencer cell lines, reciprocal expressions of Snail and Slug were applied to the reciprocal expression of LIV1 and ZIP2, both Zn ion transportor molecules on the membrane, which controls EMT intensity and differentiation of cancer cell, resulting in oral cancer progression.

Research Products

• [Journal Article] Preoperative oral health care reduces postoperative inflammation and complications in oral cancer patients. (2016)
  • Author(s): Shigeishi H, Ono S, Higashikawa K, Tada M, Ishida F, Okui G, Okumura T, Fukui A, Kubozono K, Yamamoto K, Ishida Y, Seino S, Hashikata M, Sasaki K, Naruse T, Rahman MZ, Uetsuki R, Nimiya A, Takamoto M, Dainobu K, Tokikazu T, Nishi H, Sugiyama M, Takechi M.
  • Journal Title: Exp Ther Med. Volume: 12 Issue: 3 Pages: 1922-1928
  • DOI: 10.3892/etm.2016.3532
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Semi-stable EMT型口腔癌細胞における上皮幹細胞特性の解析 (2017)
  • Author(s): 植月 亮，東川晃一郎，重石英生，石田扶美，小野重弘，島末 洋，武知正晃
  • Organizer: 第62回（公社）日本口腔外科学会総会・学術大会
• [Presentation] 口腔扁平上皮癌細胞における幹性とEMTとの関連性についてのin vitro解析 (2016)
  • Author(s): 植月 亮，東川晃一郎，重石英生，石田扶美，小野重弘，島末 洋，武知正晃
  • Organizer: 第61回(公社)日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張
  • Year and Date: 2016-11-25
• [Presentation] Snail-induced EMT ignores epithelial stem cell-like properties of oral squamous cell carcinoma cells (2016)
  • Author(s): Uetsuki R, Higashikawa K, Shigeishi H, Ishida F, Ono S, Shimasue H, Ohta K, Takechi M
  • Organizer: 第49回広島大学歯学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2016-07-02