Analysis of cellular endocrinological properties of CD133-positive oral cancer-derived sphere in a serum-free suspension culture system
Project/Area Number |
15K11251
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Hiroshima University |
Principal Investigator |
Toratani Shigeaki 広島大学, 医歯薬保健学研究科(歯), 准教授 (90172220)
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Co-Investigator(Kenkyū-buntansha) |
岡本 哲治 広島大学, 医歯薬保健学研究科(歯), 教授 (00169153)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 口腔がん / がん幹細胞 / CD133 / 口腔癌 / 無血清浮遊培養 / 癌幹細胞 |
Outline of Final Research Achievements |
In this study, we intended to develop a novel diagnostic and therapeutic method targeting cancer stem cells of human oral cancer and performed cellular and molecular biological characteristics analysis of CD133 positive cell group isolated from oral squamous cell carcinoma cell (OSCC) in serum - free culture system. As a result, there was a transit mechanism from CD133 positive cells to negative cells or from CD133 negative cells to positive cells in OSCC. EGF and SHH were thought to promote transit from CD133 negative cells to positive cells. On the other hand, anticancer agent and hypoxic environment targeted only CD133 negative cells, and transit from negative cells to positive cells was considered not to be inhibited. It seems that the EGF and SHH pathways play an important role in maintaining the function of the positive cell OSCC.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Eldecalcitol (ED-71), an analog of 1α,25(OH)2D3, inhibits the growth of squamous cell carcinoma (SCC) cells in vitro and in vivo by down-regulating expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) and FGF-22017
Author(s)
T. Shintani, F. Takatsu, S. N. Z. Rosli, E. Usui, Atsuko Hamada, K. Sumi, Y. Hayashido, S. Toratani, Tetsuji Okamoto
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Journal Title
In Vitro Cellular & Developmental Biology - Animal
Volume: 53
Issue: 9
Pages: 810-817
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation of cleidocranial dysplasia-specific human pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2016
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Pages: 252-264
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comparison of the prognosis of bisphosphonate-related osteonecrosis of the jaw caused by oral and intravenous bisphosphonates.2015
Author(s)
Shintani T, Hayashido Y, Mukasa H, Akagi E, Hoshino M, Ishida Y, Hamana T, Okamoto K, Kanda T, Koizumi K, Yoshioka Y, Tani R, Toratani S, Okamoto T
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Journal Title
Int J Oral Maxillofac Surg
Volume: in press
Issue: 7
Pages: 125-133
DOI
Related Report
Peer Reviewed / Open Access
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