Functions of a novel hypoxia-inducible reprogramming factor GLIS1 in cancer cells
Project/Area Number |
15K11252
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Hiroshima University |
Principal Investigator |
Tanimoto Keiji 広島大学, 原爆放射線医科学研究所, 助教 (90335688)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | がん / 低酸素 / 遺伝子 / がん幹細胞 / がん細胞分化 / hypoxia / 細胞分化 / 転写制御 |
Outline of Final Research Achievements |
GLI-similar 1 (GLIS1) is important for the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs). However, the molecular mechanisms of regulation of GLIS1 expression and its function remain unclear. We previously demonstrated that GLIS1 expression was dramatically increased under hypoxic conditions by HIF-2α cooperating with AP-1 family members in cancer cells. We thus conducted this study to analyze GLIS1 functions in cancer cells. As results of functional analyses by using GLIS1-overexpressing cells or inhibition with siRNA, GLIS1 was found to inhibit cell proliferation, but increase cell migration and sphere formation capacity in cancer cells. Comprehensive gene expression analyses indicated that GLIS1 regulated tissue remodeling, EMT, adipogenesis, glycolysis, and so on. Further inhibitor of DNA methylation (5-azaC) treatment increased expressions of GLIS1 in various cancer cell lines, suggesting epigenetic regulation in cancer cells.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] A chemical modulator of p53 transactivation that acts as a radioprotective agonist.2018
Author(s)
Akinori Morita, Ippei Takahashi, Megumi Sasatani, Shin Aoki, Bing Wang, Shinya Ariyasu, Kaoru Tanaka, Tetsuji Yamaguchi, Akiko Sawa, Yurie Nishi, Tatsuro Teraoka, Shohei Ujita, Yosuke Kawate, Chihiro Yanagawa, Keiji Tanimoto, Atsushi Enomoto, Mitsuru Nenoi, Kenji Kamiya, Yasushi Nagata, Yoshio Hosoi and Toshiya Inaba
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Journal Title
Molecular Cancer Therapeutics
Volume: 17
Issue: 2
Pages: 432-442
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Interactive effects of cell therapy and rehabilitation realize the full potential of neurogenesis in brain injury model2013
Author(s)
Imura T., Matsumoto M., Fukazawa T., Khalesi E., Sun Y.N., Takeda M., Uwatoko H., Nakata K., Tanimoto K., Kajiume T., Kawahara Y., Yuge L
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Journal Title
Neurosci Lett
Volume: 555
Pages: 73-78
DOI
Related Report
Peer Reviewed / Open Access