| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
GLI-similar 1 (GLIS1) is important for the reprogramming of fibroblasts into induced pluripotent stem cells (iPSCs). However, the molecular mechanisms of regulation of GLIS1 expression and its function remain unclear. We previously demonstrated that GLIS1 expression was dramatically increased under hypoxic conditions by HIF-2α cooperating with AP-1 family members in cancer cells. We thus conducted this study to analyze GLIS1 functions in cancer cells. As results of functional analyses by using GLIS1-overexpressing cells or inhibition with siRNA, GLIS1 was found to inhibit cell proliferation, but increase cell migration and sphere formation capacity in cancer cells. Comprehensive gene expression analyses indicated that GLIS1 regulated tissue remodeling, EMT, adipogenesis, glycolysis, and so on. Further inhibitor of DNA methylation (5-azaC) treatment increased expressions of GLIS1 in various cancer cell lines, suggesting epigenetic regulation in cancer cells.
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