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Mechanisms involved in inhibition of osteoclast formation by beta-glucan

Research Project

Project/Area Number 15K11263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKyushu Dental College

Principal Investigator

Ariyoshi Wataru  九州歯科大学, 歯学部, 准教授 (40405551)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords破骨細胞 / curdlan / dectin-1 / オートファジー / spleen tyrosine kinase / エンドサイトーシス / PI3-kinase / cudrlan
Outline of Final Research Achievements

In the previous study, we have demonstrated that the dectin-1 agonsist curdlan inhibits osteoclast formation through autophagy-induced Syk protein degradation.
To clarify the detailed molecular mechanisms of curdlan on osteoclast formation, we examined the Syk expression in dectin-1 over expressing RAW264.7 cells (d-RAWs) and observed the time-dependent Syk protein degradation in the presence of curdlan. Pretreatment with the autophagy/lysosome inhibitor, bafilomycin A1 markedly blocked the Syk degradation induced by curdlan. We also found that curdlan stimulation increased and autolysosome formation and the expression of autophagy marker, LC3-II. These results indicate that autophagy plays an important role in inhibition of osteoclast formation by curdlan via degradation of Syk protein. Furthermore, present study revealed that activation of phosphoinositide 3-kinase and internalization of curdlan-dencti-1 complex are required for the curdlan-induced syk degradation in d-RAWs.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Mechanisms involved in enhancement of osteoclast formation by activin-A2018

    • Author(s)
      Kajita T, Ariyoshi W, Okinaga T, Mitsugi S, Tominaga K, Nishihara T
    • Journal Title

      Journal of Cellular Biochemistry

      Volume: in print

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High molecular weight hyaluronic acid regulates MMP13 expression in chondrocytes via DUSP10/MKP52017

    • Author(s)
      Furuta J, Ariyoshi W, Okinaga T, Takeuchi J, Mitsugi S, Tominaga K, Nishihara T.
    • Journal Title

      J Orthop Res

      Volume: 35 Issue: 12 Pages: 331-339

    • DOI

      10.1002/jcp.25809

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ameloblastin and enamelin prevent osteoclast formation by suppressing RANKL expression via MAPK signaling pathway2017

    • Author(s)
      Chaweewannakorn W, Ariyoshi W, Okinaga T, Morikawa K, Saeki K, Maki K, Nishihara T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 485 Issue: 3 Pages: 621-626

    • DOI

      10.1016/j.bbrc.2017.01.181

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] High molecular weight hyaluronic acid regulates MMP13 expression in chondrocytes via DUSP10/MKP52016

    • Author(s)
      Furuta J, Ariyoshi W, Okinaga T, Takeuchi J, Mitsugi S, Tominaga K, Nishihara T
    • Journal Title

      J Orthop Res

      Volume: in print Issue: 2 Pages: 331-339

    • DOI

      10.1002/jor.23266

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Activin-A Induces Osteoclast Differentiation in Murine Bone Marrow Cultures2017

    • Author(s)
      Kajita Tomonari, Wataru Ariyoshi、Toshinori Okinaga、Tatsuji Nishihara
    • Organizer
      IADR/AADR/CADR General Session & Exhibition
    • Place of Presentation
      San Francisco, Calif, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Activin-A promotes RANKL-induced osteoclast differentiation2017

    • Author(s)
      Tomonari Kajita, Wataru Ariyoshi, Toshinori Okinaga, Sho Mitsugi, Kazuhiro Tominaga, Tatsuji Nishihara
    • Organizer
      Asia-Pacific Conference in Fukuoka 2017- International Symposium on Oral Education and Research in Kitakyushu
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mecanisms involved in inhibition of tnf-α-induced mmp13 expression by high molecular weight hyaluronic acid2017

    • Author(s)
      Wataru Ariyoshi, Toshinori Okinaga, Jun Takeuchi, Tatsuji Nishihara
    • Organizer
      Hyaluronan 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 破骨細胞の分化過程におけるActivin-Aの誘導機能の解明2017

    • Author(s)
      梶田倫功、有吉 渉、沖永敏則、三次 翔、冨永和宏、西原達次
    • Organizer
      第77回九州歯科学会総会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The Biological Effects of Ameloblastin on Osteoclast Differentiation2017

    • Author(s)
      Wichida Chaweewannakorn、有吉 渉、沖永敏則、牧 憲司、西原達次
    • Organizer
      第55回日本小児歯科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Curdlanはsykの分解を介して、破骨細胞分化を抑制する2017

    • Author(s)
      有吉 渉、西原達次
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Activin はRANKL 誘導の破骨細胞分化を亢進する2017

    • Author(s)
      梶田倫功、有吉 渉、沖永敏則、西原達次
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] cudlan-dectin-1 の相互作用により誘導されるsykの分解を介した破骨細胞分化制御機構2017

    • Author(s)
      有吉 渉、沖永敏則、西原達次
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biological Effects of Enamel 023 Related Proteins (ERPs) on Bone Metabolism in Human Being2016

    • Author(s)
      Wichida Chaweewannakorn、Kenshi Maki、Wataru Ariyoshi、Toshinori Okinaga、Tatsuji Nishihara
    • Organizer
      第23回日本歯科医学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Ameloblastin and enamelin regulate osteoclastogenesis by modifying RANKL expression via p38 and Erk1/2 MAPKs signalling pathways2016

    • Author(s)
      Chaweewannakorn Wichida、牧憲司、有吉 渉、沖永敏則、西原達次
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Interleukin-33(IL-33)によるmatrix metalloproteinase-9(MMP-9)発現増強メカニズムの解明2016

    • Author(s)
      有吉 渉、Chaweewannakorn Wichida、沖永敏則、西原達次
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Interelukin-33 up-regulates matrix metalloproteinase-9 expression via ERK-CREB-AP-1 axis2016

    • Author(s)
      Wataru Ariyoshi、Wichida Chaweewannakorn、Toshinori Okinaga、Tatsuji Nishihara
    • Organizer
      IADR/APR General Session & Exhibition
    • Place of Presentation
      Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] High molecular weight hyaluronic acid regulates MMP13 expression in chondrocytes via DUSP10/MKP52016

    • Author(s)
      J. Furuta, W. Ariyoshi, T. Okinaga, S. Mitsugi, K. Tomonaga, T. Nishihara
    • Organizer
      Interdisciplinary Medical, Dental and Soft-material Researches on the move –Showcase Review in Kitakyushu
    • Place of Presentation
      Kitakyushu
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanisms involved in regulation of osteoclastogenesis by curdlan2016

    • Author(s)
      W. Ariyoshi
    • Organizer
      Interdisciplinary Medical, Dental and Soft-material Researches on the move –Showcase Review in Kitakyushu
    • Place of Presentation
      Kitakyushu
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Curdlan誘導オートファジーによるsykの分解を介した破骨細胞分化制御機構2015

    • Author(s)
      有吉 渉、沖永敏則、西原達次
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒアルロン酸は生物学的活性によりC28/I2細胞のMMP13発現を抑制する2015

    • Author(s)
      古田絢也、沖永敏則、有吉 渉、西原達次
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Curdlanによる破骨細胞分化抑制メカニズムの解明2015

    • Author(s)
      有吉 渉、沖永 敏則、西原 達次
    • Organizer
      第26回日本生体防御学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒト軟骨細胞株C28/I2におけるTNFα 誘導MMP13発現に対するヒアルロン酸の影響2015

    • Author(s)
      古田絢也、三次翔、金氏毅、沖永敏則、吉賀大午、宮本郁也、有吉渉、吉岡泉、冨永和宏、西原達次
    • Organizer
      第75回九州歯科学会総会学術大会
    • Place of Presentation
      北九州
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Remarks] 九州歯科大学 感染分子生物学分野 ホームページ

    • URL

      http://www.kyu-dent.ac.jp/research/lecture/infection_molecule

    • Related Report
      2017 Annual Research Report
  • [Remarks] 九州歯科大学 感染分子生物学分野

    • URL

      http://www.kyu-dent.ac.jp/research/lecture/infection_molecule

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-12-27  

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