Investigation of new treatment management to try to prevent progression and exacerbation of oral mucositis

• Project/Area Number: 15K11275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: Fumihiko Yoshino 神奈川歯科大学, 大学院歯学研究科, 准教授 (20308307)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口内炎 / 活性酸素 / 漢方薬 / 化学療法 / 茵蔯蒿湯 / 半夏瀉心湯 / 黄連湯 / 抗酸化作用 / 抗酸化物質 / ファイトケミカル / 抗癌剤

Outline of Final Research Achievements

The aim of this research project is to analyze the effect on prevention / progression of oral mucositis accompanying cancer chemotherapy by antioxidant pretreatment. Alphal-glucosyl-hesperidin which is a water-soluble antioxidant substance and Chinese herbal medicine which is indicated for efficacy to oral mucositis, Hange-shashin-to/Inchinko-to/Oren-to indicated the satisfactory reactive oxygen species scavenging activity. In addition, exacerbation of oral mucositis was significantly suppressed by administering these antioxidants in vivo to experimental animals before prescribing chemotherapy. However, although the maximum area of oral mucositis caused as compared with the control was suppressed, shortening of the recovery period was not observed. From now on, it is necessary to combine with lipid-soluble antioxidants and others to aim for recovery of treatment period.

Research Products

• [Journal Article] Bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications (2017)
  • Author(s): Ibi H, Hayashi M, Yoshino F, Tamura M, Yoshida A, Kobayashi Y, Shimizu K, Lee M-C, Imai K, Ogiso B
  • Journal Title: Microbial Pathogenesis Volume: 103 Pages: 65-70
  • DOI: 10.1016/j.micpath.2016.12.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antimicrobial effect of blue light using Porphyromonas gingivalis pigment (2017)
  • Author(s): Yoshida Ayaka、Sasaki Haruka、Toyama Toshizo、Araki Mitsunori、Fujioka Jun、Tsukiyama Koichi、Hamada Nobushiro、Yoshino Fumihiko
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 5225-5225
  • DOI: 10.1038/s41598-017-05706-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antimicrobial effect of titanium dioxide after ultraviolet irradiation against periodontal pathogen (2016)
  • Author(s): Shirai R, Miura T, Yoshida A, Yoshino F, Ito T, Yoshinari M, Yajima Y
  • Journal Title: Dental Materials Journal Volume: 35 Issue: 3 Pages: 511-516
  • DOI: 10.4012/dmj.2015-406
  • NAID: 130005155189
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP). (2016)
  • Author(s): Funaki S, Tokutomi F, Wada-Takahashi S, Yoshino F, Yoshida A, Maehata Y, Miyamoto C, Toyama T, Sato T, Hamada N, Lee M-C, Takahashi S-S
  • Journal Title: Microbial Pathogenesis Volume: 92 Pages: 36-42
  • DOI: 10.1016/j.micpath.2015.12.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] α-glucosyl hesperidin suppressed the exacerbation of 5-fluorouracil-induced oral mucositis in the hamster cheek pouch. (2016)
  • Author(s): Yoshino F, Yoshida A, Toyama T, Wada-Takahashi S, Takahashi S-S
  • Journal Title: Journal of Functional Foods Volume: 21 Pages: 223-231
  • DOI: 10.1016/j.jff.2015.12.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Protective effects of (6R)-5,6,7,8-tetrahydro-L-biopterin on local ischemia/reperfusion-induced suppression of reactive hyperemia in rat gingiva (2016)
  • Author(s): Tanaka Y, Toyama T, Wada-Takahashi S, Sasaki H, Miyamoto C, Maehata Y, Yoshino F, Yoshida A, Takahashi S-S, Watanabe K, Lee M-C, Todoki K, Hamada N
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Issue: 1 Pages: 69-75
  • DOI: 10.3164/jcbn.15-69
  • NAID: 130005116629
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Blue light irradiation-induced oxidative stress in vivo via ROS generation in rat gingival tissue. (2015)
  • Author(s): Yoshida A, Shiotsu-Ogura Y, Wada-Takahashi S, Takahashi S-S, Toyama T, Yoshino F
  • Journal Title: Journal of Photochemistry and Photobiology B: Biology Volume: 151 Pages: 48-53
  • DOI: 10.1016/j.jphotobiol.2015.07.001
  • Peer Reviewed / Open Access
• [Journal Article] Relationship among salivary antioxidant activity, cytokines and periodontitis: the Nagasaki Island study. (2015)
  • Author(s): Tamaki N, Yoshino F, Fukui M, Hayashida H, Yoshida A, Kitamura M, Iwasaki T, Furugen R, Kawasaki K, Nakazato M, Maeda T, Kokeguchi S, Yamamoto T, Lee M-C, Ito H, Saito T
  • Journal Title: Journal of Clinical Periodontology Volume: 42 Issue: 8 Pages: 711-718
  • DOI: 10.1111/jcpe.12438
  • Peer Reviewed
• [Presentation] The Effect of α-glucosyl Hesperidin on Oral Mucositis Induced by Treatment of 5-fluorouracil with Acetic Acid. (2017)
  • Author(s): Yoshino F, Yoshida A,Toyama T
  • Organizer: CED-IADR/NOF Oral Health Research Congress
  • Int'l Joint Research
• [Presentation] The inhibition mechanism of gingival fibroblast proliferation by blue-light irradiation. (2017)
  • Author(s): Yoshida A, Yoshino F
  • Organizer: CED-IADR/NOF Oral Health Research Congress
  • Int'l Joint Research
• [Presentation] マウス実験的歯周病炎モデルにおける生薬「鶏血藤」の循環改善効果 (2017)
  • Author(s): 遠山歳三，塗々木和男，高橋俊介，吉野文彦，吉田彩佳，松尾雅斗，高橋聡子，佐々木 悠，稲葉啓太郎，渡辺清子，浜田信城
  • Organizer: 日本歯周病学会 60 周年記念京都大会
• [Presentation] Antibacterial Activity of Infrared Free Electron Laser on Cariogenic Bacteria (2017)
  • Author(s): oyama T, Fujioka J, Koyata Y, Sakuma T, Watanabe K, Sasaki H, Yoshino F, Yoshida A, Tsukiyama K, Hamada N
  • Organizer: 95th General session & Exhibition of the IADR, 46th Annual meeting of the AADR and 41th Annual meeting of the CADR
  • Int'l Joint Research
• [Presentation] Study of Blue-light Effect on rat palatal gingiva. (2016)
  • Author(s): Yoshida A, Yoshino F
  • Organizer: 45th Annual meeting of the AADR
  • Place of Presentation: Los Angeles, CA, USA.
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] 糖転移ヘスペリジンの難治性口内炎に対する増悪改善効果. (2016)
  • Author(s): 吉野文彦
  • Organizer: 糖転移ヘスペリジン・ビタミンP研究会
  • Place of Presentation: 東京都千代田区
  • Invited
• [Presentation] ガン化学療法に併発する口内炎に対する糖転移ヘスペリジンの効果 (2016)
  • Author(s): 吉野文彦，吉田彩佳，小椋有香子，遠山歳三，高橋聡子，高橋俊介
  • Organizer: 神奈川歯科大学学会 第 51 回総会
  • Place of Presentation: 神奈川県横須賀市
• [Remarks] 神奈川歯科大学大学院口腔科学講座光歯科医学
  • URL: http://www.labs.kdu.ac.jp/pmd/