Explication of carcinogenesis related gene using mapping array of oral cancer and precancerous region

• Project/Area Number: 15K11278
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Dental College (2017); Kanagawa Dental College (2015-2016)
• Principal Investigator: Yamamoto Nobuharu 東京歯科大学, 歯学部, 講師 (60385185)
• Co-Investigator(Kenkyū-buntansha): 柴原 孝彦 東京歯科大学, 歯学部, 教授 (50178919) ; 恩田 健志 東京歯科大学, 歯学部, 講師 (30433949)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔癌 / 白板症 / DNAマッピングアレイ / 癌抑制遺伝子 / ヘテロ接合性消失 / ヘテロ接合生消失

Outline of Final Research Achievements

Were histopathological, and analyzed the DNA which we obtained from an oral squamous cell carcinoma tissue of regional node-positive case pN(+) and negative case pN(-) by Mapping Array, and detected genome copy number abnormality to be common to a pN(+) case as compared with a pN(-) case, and to be found, and listed candidate metastasis-related gene which was present in theatrical company rank. As a result of Real-time PCR, an expression of Sirt1 mRNA decrease was found in 47.1% of oral squamous cell carcinoma tissue as compared with a normal oral tissue of the resection stump neighborhood.
Sirt1 had the tendency indicating the expression decrease in a pN(+) case frequently.The likelihood that Sirt1 played an important role in in progress and lymph node metastases of oral squamous cell carcinoma was suggested.

Research Products

• [Journal Article] Treatment of mandibular reconstruction by free vascularized fibular transplant experiment in our department (2018)
  • Author(s): Yamamoto, N., Morikawa, T., Yakushiji, T., Shibahara, T.
  • Journal Title: The Bulletin Tokyo Dental College Volume: in press
  • Peer Reviewed
• [Journal Article] Malignant transformation of oral leukoplakia with a focus on low-grade dysplasia. (2016)
  • Author(s): Watabe, Y., Nomura, T., Onda, T., Yakushiji, T., Yamamoto, N., Ohata, H., Takano, N., Shibahara, T.
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 28 Pages: 26-29
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular biological change in oral cancer, summary of our researches. (2015)
  • Author(s): Yamamoto, N., Onda, T., Sugahara, K., Nomura, T., Shibahara, T.
  • Journal Title: Japanese Dental Science Review Volume: 51 Pages: 25-33
  • NAID: 50009645831
  • Peer Reviewed / Open Access
• [Journal Article] Intelligibility of medical terms in students at School of Dentistry, Kanagawa Dental University. (2015)
  • Author(s): Yamamoto, N., Yamamoto, T., Kubota, M., Hanaoka, K., Sugaya, A., Yuyama, N., Hayashida, J., Kimura, K., Kumada, H., Yi, J., Sakai, N., Kawakami, M., Sakurai, T., Tsukinoki, K., Hirata, Y.
  • Journal Title: Kanagawa Shigaku Volume: 50 Pages: 73-81
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A case of lower gingival carcinoma with drug-induced interstitial pneumonia caused after chemotherapy with peplomycin. (2015)
  • Author(s): Yamamoto, N., Fuse, Y., Yoshida, Y., Suzuki, T., Noguchi, S., Katakura, A., Tanaka, Y., Shibahara, T., Takano, N.
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 27 Pages: 92-95
  • Peer Reviewed / Open Access
• [Journal Article] A case of lower gingival squamous cell carcinoma of perineural spread to cavernous sinus and orbit caused by mandibular nerve invasion. (2015)
  • Author(s): Yamamoto, N., Fuse, Y., Yoshida, Y., Katakura, A., Tanaka, Y., Shibahara, T., Takano, N.
  • Journal Title: Japanese Journal of Oral Diagnosis/Oral Medicine Volume: 28 Pages: 147-152
  • Peer Reviewed / Open Access
• [Presentation] 口腔白板症に対する治療方針決定に関する臨床的検討 (2018)
  • Author(s): 小山拓洋、笠原清弘、西山明宏、大野啓介、菅原圭亮、別所央城、山本信治、中島 啓、松坂賢一、高野正行、齊藤 力、柴原孝彦、片倉 朗
  • Organizer: 第36回日本口腔腫瘍学会総会・学術大会
• [Presentation] Stage 1、2舌扁平上皮癌におけるTumor buddingの臨床的意義の検討 (2018)
  • Author(s): 長谷川大悟、前山恵里、関川翔一、大野啓介、吉田秀児、山本信治、中島 啓、松坂賢一、高野正行、齊藤 力、柴原孝彦
  • Organizer: 第36回日本口腔腫瘍学会総会・学術大会
• [Presentation] 当科を受診した口腔がん患者の臨床的検討～Tumor buddingによる予後予測～ (2017)
  • Author(s): 前山恵里、大野啓介、住吉美咲、大金 覚、別所央城、山本信治、高野正行、片倉 朗、齊藤 力、柴原孝彦
  • Organizer: 第62回日本口腔外科学会総会
• [Presentation] 東京歯科大学水道橋病院口腔外科における2016年度初診患者の臨床検討 (2017)
  • Author(s): 辛 麻由、加藤禎彰、小山拓洋、布施 俊、住吉美咲、西山明宏、関根理予、大野啓介、吉田秀児、高木 亮、菅原圭亮、別所央城、渡邊 章、山本信治、笠原清弘、高野正行、齊藤 力、柴原孝彦、片倉 朗
  • Organizer: 第304回東京歯科大学学会（総会）
• [Presentation] 新たな蛍光観察装置（ORALOOK）を用いた口腔粘膜疾患に対する使用経験 (2017)
  • Author(s): 山本信治、大野啓介、吉田秀児、高野正行、柴原孝彦
  • Organizer: 第3回日本顎顔面再建先進デジタルテクノロジー学会総会
• [Presentation] Dental Lab Systemの改変と脱Model Surgeryへの挑戦 (2017)
  • Author(s): 吉田秀児、小高研人、海老澤朋宏、安村敏彦、大野啓介、高梨琢也、渡邊 章、山本信治、松永 智、西井 康、齊藤 力、阿部伸一、末石研二、柴原孝彦
  • Organizer: 第3回日本顎顔面再建先進デジタルテクノロジー学会総会
• [Presentation] 口腔粘膜疾患に対する新たな蛍光観察装置（イルミスキャン）の開発 (2015)
  • Author(s): 山本信治
  • Organizer: 第60回日本口腔外科学会総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-16
  • Invited
• [Presentation] 個別検診をサポートする口腔がん検診ナビシステム (2015)
  • Author(s): 別所央城、恩田健志、藥師寺孝、大金 覚、野村武史、山本信治、高野伸夫、片倉 朗、柴原孝彦
  • Organizer: 第60回日本口腔外科学会総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-16
• [Presentation] 口腔粘膜疾患に対する新たな蛍光観察装置（イルミスキャン）の検討 (2015)
  • Author(s): 山本信治、菅原圭亮、柴原孝彦
  • Organizer: 第8回日本口腔検査学会総会
  • Place of Presentation: 神奈川歯科大学（神奈川県横須賀市）
  • Year and Date: 2015-10-03
• [Book] 口腔粘膜疾患の診断の助けとなる口腔内蛍光観察装置 (2018)
  • Author(s): 山本信治、柴原孝彦
  • Publisher: デンタルダイヤモンド
• [Book] JMEDJ治療法便覧 私の治療 2017-18年度版 19.歯科・口腔外科疾患 (2017)
  • Author(s): 山本信治
  • Publisher: 日本医事新報社
• [Book] Oral cancer -Epidemiology of the oral cancer- (2015)
  • Author(s): Yamamoto, N.（分担執筆）
  • Total Pages: 426
  • Publisher: Springer, Tokyo