| Project/Area Number |
15K11281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
水越 堅詞 朝日大学, 歯学部, 助教 (90631565)
佐藤 慶太郎 朝日大学, 歯学部, 講師 (10549041)
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| Research Collaborator |
KASHIMATA Masanori
GRESIK Edward
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 顎下腺 / 分枝形態形成 / 上皮間葉相互作用 / Shh/Ptch / EGF/ErbB / Shh / EGF／EGF受容体 / 2 / SHH / 上皮成長因子 / EGF受容体 |
|---|
| Outline of Final Research Achievements |
The fundamental processes of epithelio-mesenchymal interactions depend both on a variety of growth factors and their receptors. Shh are well known as a morphogen that plays many important roles during development of numerous organs. In this study, we investigated the relationship between Shh/Ptch and EGF/ErbB signals of developing fetal mouse SMG. Shh stimulated BrM and induced phosphorylations of ErbB1 and ERK1/2 in SMG rudiments. Shh also significantly induced mRNA syntheses for EGF ligand and ErbB family. Induction of mRNA level of Egf was specific in mesenchyme and inductions of mRNA levels for Erbb1, Erbb2 and Erbb3 were specific in epithelium of SMG rudiments. Gli1 transcription factor level was elevated by administrations of Shh to cultured SMG rudiments. These results suggested that Shh stimulates BrM of fetal mouse SMG through an activation of EGF/ErbB/ERK1/2 signaling systems, and the stimulations by Shh may be regulated by the transcription factor, Gli1.
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