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Investigation of potential use of molecular targeted medicine on inhibition of oral cancer invasion and metastasis

Research Project

Project/Area Number 15K11286
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionTsurumi Junior College

Principal Investigator

Fujihara Hisako  鶴見大学短期大学部, 歯科衛生科, 准教授 (80396746)

Co-Investigator(Kenkyū-buntansha) 川口 浩司  鶴見大学, 歯学部, 准教授 (50277951)
宮嶋 千秋  鶴見大学, 歯学部, 学部助手 (50723722)
馬杉 亮彦  鶴見大学, 歯学部, 助教 (80351922)
山田 浩之  岩手医科大学, 歯学部, 教授 (90267542)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords口腔がん / 転移 / 浸潤 / 分子標的治療薬 / 分子標的薬 / 浸潤能 / 転移能
Outline of Final Research Achievements

In this report, mechanism of oral cancer invasion and metastasis was analyzed using PARP inhibitor which is one of molecular target drugs and currently under clinical trial for breast cancer. Materials used in this reports are three kinds of oral cancer derived cells, Ca9-22, SAS, and HSC-2 and Olaparib (AZD2281) as PARP inhibitor.
In the first year, PARP inhibitor showed significant decrease of 1) cell proliferation, 2) cell migration, 3) cell infiltration, and 4) cell adhesion except cell (Ca9-22) adhesion for laminin coating, which showed significant increase of cell adhesion using with Olaparib. Next, xenografted tumors were generated by injection of tumor cells into masseter muscles. Tumor volumes of control group mice increased during the experimental period. However, tumor growth rate of AZD2281 group significantly decreased compared to the control group after tumor cell injection. Xenografted tumor invasion to mandibule was also compared between Olaparib/control groups.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Detection accuracy for epithelial dysplasia using an objective autofluorescence visualization method based on the luminance ratio2017

    • Author(s)
      Yamamoto Nanami、Kawaguchi Koji、Fujihara Hisako、Hasebe Mitsuhiko、Kishi Yuta、Yasukawa Masaaki、Kumagai Kenichi、Hamada Yoshiki
    • Journal Title

      International Journal of Oral Science

      Volume: 9 Issue: 11 Pages: e2-e2

    • DOI

      10.1038/ijos.2017.37

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synergetic Effects of PARP Inhibitor AZD2281 and Cisplatin in Oral Squamous Cell Carcinoma in Vitro and in Vivo2016

    • Author(s)
      Masaaki Yasukawa, Hisako Fujihara, Hiroaki Fujimori, Koji Kawaguchi, Hiroyuki Yamada, Ryoko Nakayama, Nanami Yamamoto, Yuta Kishi, Yoshiki Hamada, Mitsuko Masutani.
    • Journal Title

      International Journal of Moeular Sciences

      Volume: 2016 Issue: 3 Pages: 272-272

    • DOI

      10.3390/ijms17030272

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway.2015

    • Author(s)
      Yuta Kishi, Hisako Fujihara, Koji Kawaguchi, Hiroyuki Yamada, Ryoko Nakayama, Nanami Yamamoto, Yuko Fujihara, Yoshiki Hamada, Kazuhito Satomura, Mitsuko Masutani.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 16 Issue: 10 Pages: 24820-24838

    • DOI

      10.3390/ijms161024820

    • NAID

      120006986847

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 複数分野の専門医による地域連携型口腔検診確立の試み2015

    • Author(s)
      寺田 知加, 花谷 重守, 藤原 久子, 太田 増美, 井出 信次, 内田 直貴, 杉田 智樹, 服部 環, 矢内 啓郎, 山本 早希子, 近藤 千晴, 小島 充貴, 朝田 芳信, 里村 一人
    • Journal Title

      日本歯科人間ドック学会誌

      Volume: 10 Pages: 13-18

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] ポリADP-リボシル化反応はマウス間葉系間細胞(MSC)の骨芽細胞分化に関与する2016

    • Author(s)
      岸悠太,藤原久子,山本那々美, 山田浩之,川口浩司,濱田良樹
    • Organizer
      第61回 日本口腔外科学会総会・学術大会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 口腔扁平上皮がん細胞に対するシスプラチンとPARP阻害剤AZD2281の相乗効果2016

    • Author(s)
      安川仁章, 藤原久子, 藤森浩彰, 川口浩司, 山田浩之, 中山亮子, 山本那々美, 岸悠太, 濱田良樹
    • Organizer
      第61回 日本口腔外科学会総会・学術大会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Yamamoto N, Kawaguchi K, Fujihara H, Yasukawa M, Kishi Y, Hasebe M, Kumagai K, Hamada Y2015

    • Author(s)
      Yamamoto N, Kawaguchi K, Fujihara H, Yasukawa M, Kishi Y, Hasebe M, Kumagai K, Hamada Y
    • Organizer
      AAOMS(American Association of Oral and Maxillofacial Surgery) 97th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 医事法講座第7巻 小児医療と医事法 第11章 小児歯科をめぐる諸問題2016

    • Author(s)
      藤原久子(甲斐克則編)
    • Total Pages
      282
    • Publisher
      信山社
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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