Functional analysis of MYEOV gene in oral squamous cell carcinoma

Research Project

Project/Area Number	15K11291
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Surgical dentistry
Research Institution	Yamaguchi University
Principal Investigator	UCHIDA Kenichiro 山口大学, 医学部附属病院, 助教 (20379986)
Co-Investigator(Kenkyū-buntansha)	竹縄隆徳山口大学, 医学部, 特別医学研究員 (30711270)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	癌 / 遺伝子
Outline of Final Research Achievements	We identified overexpression of MYEOV gene in oral squamous cell carcinoma. Amplification and overexpression of the MYEOV gene has also been reported in other cancers. However, its role is still unknown. The aim of this study is to screen the alteration of gene expression under the RNA interference of MYEOV gene and to clarify the role of MYEOV gene in the development and progression of oral squamous cell carcinoma. We performed knock down (KD) of the MYEOV gene and investigated changes in gene expression by RNA sequencing and real time PCR. As a result, expression of ERO1LB gene and KRT6A gene decreased in the oral cancer cell line. In addition, KD of MYEOV showed suppression of cell proliferation and colony formation.