New strategy for oral cancer treatment by control of long non-coding RNA
Project/Area Number |
15K11300
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Nara Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
國安 弘基 奈良県立医科大学, 医学部, 教授 (00253055)
桐田 忠昭 奈良県立医科大学, 医学部, 教授 (70201465)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | Oral cancer / long non-coding RNA / malic enzyme / 口腔がん / 長鎖non-codingRNA / 口腔癌 / 長鎖ncRNA / 転移 / 浸潤 / 口腔扁平上皮癌 |
Outline of Final Research Achievements |
Malic enzyme 1 (ME1) is a multifunctional protein involved in energy production and overexpressed in various cancers. We examined expression and function of ME1 in oral squamous cell carcinomas (OSCC). Immunohistochemical expression of ME1 was moderated to strong in 48% of OSCCs and correlated with pT, pN clinical stage and histological grade. Moderate to strong ME1 expression indicated a worse prognosis than did weak ME1 expression. Malic enzyme 1 knockdown or inactivation by lanthanide inhibited cell proliferation and mortility and suppressed the epithelial-mesenchymal transition in HSC3 human OSCC cells. Knockdown of ME1 also shifted energy metabolism from aerobic glycolysis and lactate fermentation to mitochondrial oxidative phosphorylation and the redox status from reductive to oxidative. In a mouse tumor model, lanthanide suppressed tumor growth and increased survival time. These findings reveal that ME1 is a valid target for molecular therapy in OSCC.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Expression of cytosolic malic enzyme (ME1) is associated with disease progression in human oral squamous cell carcinoma.2018
Author(s)
Nakashima C, Yamamoto K, Katsuragawa H, Shimomura M, Fujiwara-Tani R, Luo Y, Matsushima S, Fujii K, Ohmori H, Sasaki T, Sasahira T, Kirita T
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Journal Title
Related Report
Peer Reviewed / Open Access
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[Presentation] HuD promotes progression of oral squamous cell carcinoma2015
Author(s)
Kurihara M, Sasahira T, Yamamoto K, Kuniyasu H, Kirita T
Organizer
The Joint Meeting of 4th Congress of Asian Society of Head and Neck Oncology & 39th Annual Meeting of Japan Society for Head and Neck Cancer
Place of Presentation
Kobe
Year and Date
2015-06-03
Related Report
Int'l Joint Research