Development of a novel general anesthetic method applying the enhancement of descending pain suppression system: Utilization of 5-HT receptor ligands
| Project/Area Number |
15K11313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Irifune Masahiro 広島大学, 医歯薬保健学研究科(歯), 教授 (10176521)
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| Co-Investigator(Kenkyū-buntansha) |
兼松 隆 広島大学, 医歯薬保健学研究科(歯), 教授 (10264053)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | サブスタンスP / 脊髄後角組織 / イミプラミン / モルヒネ / プロポフォール / ケタミン / マイクロダイアリシス法 / 脊髄後根神経節培養細胞 / 脊髄後角組織スライス / セロトニン受容体リガンド / オピオイド受容体 / サブスタンスＰ / セロトニン受容体 / 不動化 |
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| Outline of Final Research Achievements |
Whether the selective serotonin receptor ligands inhibit or whether the general anesthetics enhance substance P (SP) release from cultured rat dorsa root ganglion (DRG) neurons or rat spinal cord chopped tissues was examined. Capsaicin increased SP release from cultured DRG cells in a concentration-dependent fashion. The intravenous general anesthetics propofol and ketamine and the opioid receptor agonist morphine did not affect the capsaicin-induced SP release. Also, capsaicin concentration-dependently increased SP release from rat spinal cord chopped tissues. Morphine concentration-dependently inhibited the capsaicin-induced SP release. High concentrations of propofol enhanced the SP release, but ketamine did not. As the selective serotonin uptake inhibitor imipramine concentration-dependently increased the SP release, this drug did not have antinociceptive action in this region.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] General anesthetic actions on GABAA receptors in vivo are reduced in phospholipase C-related catalytically inactive protein knockout mice.2017
Author(s)
Hayashiuchi M, Kitayama T, Morita K, Yamawaki Y, Oue K, Yoshinaka T, Asano S, Harada K, Kang Y, Hirata M, Irifune M, Okada M, Kanematsu T.
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Journal Title
Journal of Anesthesia
Volume: in press
Issue: 4
Pages: 531-538
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phospholipase C-related catalytically inactive protein is a new modulator of thermogenesis promoted by β-adrenergic receptors in brown adipocytes2015
Author(s)
Kana Oue, Jun Zhang, Kae Harada-Hada, Satoshi Asano, Yosuke Yamawaki, Masaki Hayashiuchi, Hisako Furusho, Takashi Takata, Masahiro Irifune, Masato Hirata, Takashi Kanematsu
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Journal Title
Journal of Biological Chemistry
Volume: 291
Issue: 8
Pages: 4185-96
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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