Establishment of surgical treatment of medication-related osteonecrosis of the jaws using tetracycline fluorescent labeling method

Research Project

Project/Area Number	15K11321
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Surgical dentistry
Research Institution	Kyushu Dental College
Principal Investigator	Sasaguri Masaaki 九州歯科大学, 歯学部, 准教授 (00225898)
Co-Investigator(Kenkyū-buntansha)	吉賀大午九州歯科大学, 歯学部, 助教 (10507784) 松尾拡九州歯科大学, 歯学部, 教授 (70238971)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	MRONJ / ARONJ / BRONJ / ミノマイシン / 骨蛍光標識法 / テトラサイクリン / 蛍光
Outline of Final Research Achievements	Objectives: The purpose of this study was to determine the potential of tissue fluorescence imaging byusing Visually Enhanced Lesion Scope (VELscope_) for the detection of osteonecrosis of Medication related Osteonecrosis of the Jaw.MRONJ mdel rat could be made by bone drilling with LPS. MRONJ model rats ware divided into two groups; Minomycine administrated group (subcutaneous injection of Minomycin for 7 days) :M(+) andMinomycin non-sdministrated group : M(-). Bone fluorescence evaluation by VEL scope was compared between two groups. We got the impression that the contrast between the fluorescent part and the non-fluorescent part was more clear in M(+) group yhan in M(-) group. Histopathological findings of the fluorescent part was normal structure of bone, on the other hand the non-fluorescent part showed necrosis of bone pathological bone. Conclusion: VELscope examination is a suitable tool to visualize necrotic areas of the bone in MRONJ.