Cell sheets were prepared and layered to produce a hybrid cell sheet with verification of transplant

• Project/Area Number: 15K11322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Abe Shinichi 東京歯科大学, 歯学部, 教授 (40256300)
• Research Collaborator: Yamane Shigeki ; Umezawa Takashi ; Serikawa Masamitsu
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 筋芽細胞 / Desmin / IGF-1 / コラーゲン / 頬粘膜 / 口腔粘膜 / 咀嚼機能 / 嚥下機能 / 筋細胞シート / ハイブリッド型細胞シート / デスミン / ケラチン / 細胞シート / 口腔粘膜上皮 / 積層シート / 線維芽細胞 / 粘膜再生 / 筋シート / 積層細胞シート / ミオシン / 中間径フィラメント

Outline of Final Research Achievements

Cell sheets of myoblasts and mesenchymal cells were generated, and the effects of the mesenchymal cells on the myoblasts during long-term cultivation were investigated. Presence of desmin and collagen type IV seen in normal muscle tissues was also confirmed in the produced cell sheets. IGF, insulin-like growth factor, was expressed only when the mesenchymal cells and myoblasts were co-cultured, suggesting the presence of mesenchymal cells in the long-term co-culturing system could influence myoblasts proliferation. Furthermore, the cell sheet was transplanted into the rabbit lamina mucosa, and the localization of the muscle specific protein (desmin) and collagen was observed, and it was confirmed that the cell sheet was assimilated in the mucosa.

Research Products

• [Journal Article] Distance between intramuscular nerve and artery in the extraocular muscles: a preliminary immunohistochemical study using elderly human cadavers (2017)
  • Author(s): Kitamura K, Cho KH, Jang HS, Murakami G, Yamatmoto M, Abe S.
  • Journal Title: Surgical and Radiologic Anatomoy Volume: 39 Issue: 1 Pages: 3-9
  • DOI: 10.1007/s00276-016-1642-9
  • Peer Reviewed
• [Journal Article] Coccygeal body revisited: an immunohistochemical study using donated elderly cadavers. (2017)
  • Author(s): Jin ZW, Cho KH, Jang HS. Murakami G, Rodríguez-Vázquez JF, Yamamoto M, Abe S.
  • Journal Title: Anatomical Record Volume: 300 Issue: 10 Pages: 1826-1837
  • DOI: 10.1002/ar.23615
  • Peer Reviewed
• [Journal Article] Engineered three-dimensional rabbit oral epithelial-mesenchymal-muscular hybrid sheets (2016)
  • Author(s): Yamane, S., Higa, K., Umezawa, T., Serikawa, M., Shimazaki, J. and Abe, S.
  • Journal Title: International Journal of Oral Science Volume: 8 Issue: 3 Pages: 145-154
  • DOI: 10.1038/ijos.2016.16
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Proliferative activity of skeletal myoblast sheet by paracrine effects of mesenchymal stem cells (2016)
  • Author(s): Umezawa, T., Higa., M., Serikawa, M., Yamamoto, M., Matsunaga, S., Shimazaki, J. and Abe, S.
  • Journal Title: Journal of Oral Biosciences Volume: 58 Issue: 4 Pages: 158-166
  • DOI: 10.1016/j.job.2016.05.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Engineered three-dimensional rabbit oral epithelial-mesenchymal-muscular hybrid sheets (2016)
  • Author(s): Shigeki Yamane, Kazunari Higa, Takazhi Umezawa, Jun Shimazaki, Shinichi Abe
  • Journal Title: International Journal of Oral Science Volume: 8
  • Acknowledgement Compliant
• [Presentation] 上皮・間葉ハイブリッド型細胞シート合成過程に発現する細胞骨格関連タンパク (2016)
  • Author(s): 森田 純晴、松永 智、北村 啓、芹川 雅光、梅澤 貴志、阿部 伸一
  • Organizer: 第121回日本解剖学会全国学術集会
  • Place of Presentation: 郡山市
  • Year and Date: 2016-03-29
• [Presentation] コラーゲンゲルを用いた筋芽細胞シートに対する間葉系細胞の影響 (2015)
  • Author(s): 芹川 雅光，梅澤 貴志，北村 啓，森田 純晴，山本 将仁，阿部 伸一
  • Organizer: 第103回日本解剖学会関東支部学術集会
  • Place of Presentation: 横浜市北区
  • Year and Date: 2015-11-07