| Project/Area Number |
15K11331
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
Kamiya Masako 朝日大学, 経営学部, 准教授 (80181907)
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| Co-Investigator(Kenkyū-buntansha) |
近藤 信夫 朝日大学, 歯学部, 教授 (40202072)
智原 栄一 朝日大学, 歯学部, 教授 (80244581)
山崎 裕 北海道大学, 歯学研究院, 教授 (90250464)
村松 泰徳 朝日大学, 歯学部, 教授 (30247556)
高山 英次 朝日大学, 歯学部, 准教授 (70533446)
川木 晴美 朝日大学, 歯学部, 准教授 (70513670)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 麻酔薬 / 抗腫瘍免疫 / 癌組織内微小環境 / 口腔扁平上皮癌 / 免疫抑制 / 局所麻酔薬 / 静脈麻酔薬 / 免疫 / サイトカイン / 免疫応答 / 悪性腫瘍 / インターフェロンγ / 癌微小環境 |
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| Outline of Final Research Achievements |
To clarify the effects of the anesthetics on the tumor microenvironment, we evaluated their cytotoxicity against oral squamous cell carcinoma (OSCC) cells, stromal cells (10T1/2) and mouse spleen cells and the influence on immuno-response of these cells.
The all of anesthetics tested showed cytotoxic effects on spleen cells at much lower concentrations than on the OSCC cells and 10T1/2, which suggests that spleen cells are more sensitive against anesthetics than the other cell lines. The cytokine-production by anti-CD3-stimulated spleen cells was completely inhibited at a lower concentration of intravenous anesthetics, whereas spleen cells maintained a 80-100% viability. In addition, only midazolam inhibited the immunosuppressive action of 10T1/2. On the other hand, in animal experiments with tumor-bearing mice, it was suggested that intravenous anesthetics could inhibit the development of tumor.
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