Development of new bone prosthetic material using iPS cell and phosphorylated pullulan

• Project/Area Number: 15K11336
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: Seiryu Masahiro 東北大学, 歯学研究科, 助教 (80510023)
• Co-Investigator(Kenkyū-buntansha): 山本 照子 東北大学, 歯学研究科, 名誉教授 (00127250) ; 竹下 信郎 東北大学, 歯学研究科, 助教 (50431515)
• Research Collaborator: TAKUMI OKIHARA ; YASUHIRO YOSHIDA
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯学 / 再生医学 / 歯科矯正学

Outline of Final Research Achievements

The result of this study is as follows, 1) We succeeded in the induction from iPS cells of the mouse to the neural crest like cells. 2) TCP+NCLC group was shown to be stronger in a tendency to healing than TCP+MC3T3 group. 3) The CT value of the group of TCP+phosphorylated pullulan was higher than other groups. The results suggested that new bone prosthetic material may be realized by using iPS cell and phosphorylated pullulan.

Research Products

• [Journal Article] Synergistic acceleration of experimental tooth movement by supplementary high-frequency vibration applied with a static force in rats (2017)
  • Author(s): Teruko Takano-Yamamoto, Kiyo Sasaki, Goudarzi Fatemeh, Tomohiro Fukunaga, Masahiro Seiryu, Takayoshi Daimaruya, Nobuo Takeshita, Hiroshi Kamioka, Taiji Adachi, Hiroto Ida & Atsushi Mayama
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 13969-13969
  • DOI: 10.1038/s41598-017-13541-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of osteoclast differentiation and actin ring formation by the cytolinker protein plectin. (2017)
  • Author(s): Matsubara T, Kinbara M, Maeda T, Yoshizawa M, Kokabu S, Takano Yamamoto T.
  • Journal Title: Biochem Biophys Res Commun. Volume: Aug 5;489(4) Issue: 4 Pages: 472-476
  • DOI: 10.1016/j.bbrc.2017.05.174
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Underlying Mechanisms and Therapeutic Strategies for Bisphosphonate-Related Osteonecrosis of the Jaw (BRONJ) (2017)
  • Author(s): Endo Y, Kumamoto H, Nakamura M, Sugawara S, Takano-Yamamoto T, Sasaki K, Takahashi T
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 40 Issue: 6 Pages: 739-750
  • DOI: 10.1248/bpb.b16-01020
  • NAID: 130005688081
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mouse Model of Hydroquinone Hypersensitivity via Innate and Acquired Immunity and Its Promotion by Combined Reagents (2017)
  • Author(s): Bando K, Tanaka Y, Kuroishi T, Sasaki K, Takano-Yamamoto T, Sugawara S, Endo Y
  • Journal Title: Journal of Investigative Dermatology Volume: 印刷中 Issue: 5 Pages: 1082-1093
  • DOI: 10.1016/j.jid.2016.12.018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inflammatory Effects of Nitrogen-Containing Bisphosphonates (N-BPs): Modulation by Non-N-BPs (2017)
  • Author(s): Shima K, Tsuchiya M, Oizumi T, Takano-Yamamoto T, Sugawara S, Endo Y.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 40 Issue: 1 Pages: 25-33
  • DOI: 10.1248/bpb.b16-00521
  • NAID: 130005188786
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress. (2017)
  • Author(s): Takano-Yamamoto T, Fukunaga T, Takeshita N.
  • Journal Title: Methods Mol Biol. Volume: 1489 Pages: 283-308
  • DOI: 10.1007/978-1-4939-6430-7_26
  • ISBN: 9781493964284, 9781493964307
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force (2016)
  • Author(s): Takeshita N, Hasegawa M, Sasaki K, Seki D, Seiryu M, Miyashita S, Takano I, Oyanagi T, Miyajima Y, Takano-Yamamoto T
  • Journal Title: J Bone Miner Metab Volume: 印刷中 Issue: 1 Pages: 40-51
  • DOI: 10.1007/s00774-016-0737-z
  • Peer Reviewed / Open Access
• [Journal Article] In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force. (2016)
  • Author(s): Takeshita N, Hasegawa M, Sasaki K, Seki D, Seiryu M, Miyashita S, Takano I, Oyanagi T, Miyajima Y, Takano-Yamamoto T
  • Journal Title: J Bone Miner Metab Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Scleraxis and osterix antagonistically regulate tensile force-responsive remodeling of the periodontal ligament and alveolar bone. (2015)
  • Author(s): Takimoto A., Kawatsu M., Yoshimoto Y., Kawamoto T., Seiryu M., Takano-Yamamoto T., Hiraki Y., Shukunami C.
  • Journal Title: Development. Volume: 142(4) Issue: 4 Pages: 787-796
  • DOI: 10.1242/dev.116228
  • Peer Reviewed / Open Access
• [Journal Article] Decreased alveolar bone turnover is related to the occurrence of root resorption during experimental tooth movement in dogs (2015)
  • Author(s): Deguchi T, Seiryu M, Daimaruya T, Garetto LP, Takano-Yamamoto T, Roberts WE
  • Journal Title: Angle Orthod Volume: 85(3) Issue: 3 Pages: 386-393
  • DOI: 10.2319/021714-117.1
  • Peer Reviewed / Open Access
• [Journal Article] Differentiation of odontoblast-like cells from mouse induced pluripotent stem cells by Pax9 and Bmp4 transfection (2015)
  • Author(s): Seki D, Takeshita N, Oyanagi T, Sasaki S, Takano I, Hasegawa M, Takano-Yamamoto T
  • Journal Title: Stem Cells Transl Med Volume: 4(9) Issue: 9 Pages: 993-997
  • DOI: 10.5966/sctm.2014-0292
  • Peer Reviewed / Open Access
• [Presentation] Tooth movement and mechanical stress-Role of osteocytes and osteoimmune factors. (2017)
  • Author(s): Takano-Yamamoto T
  • Organizer: The 20th Annual Meeting of Taiwan Orthodontic Society,
  • Int'l Joint Research / Invited
• [Presentation] 歯科矯正用アンカースクリューを用いて、上顎歯列の正中偏位を改善した症例 (2017)
  • Author(s): 井田裕人、清流正弘、山本照子
  • Organizer: 第33回東北矯正歯科学会
• [Presentation] RUNK2+/-マウスの矯正的歯の移動の牽引側における骨芽細胞の増殖および分化気候にRunx2/mTORC2シグナルが関与する (2017)
  • Author(s): 青沼 智、福永智広、北浦英樹、竹下信郎、山城隆、山本照子
  • Organizer: 第35回日本骨代謝学会学術集会
• [Presentation] 牽引力負荷された歯根膜の初期反応におけるscleraxisの発現機序と機能の解析 (2017)
  • Author(s): 川津正慶、竹下信郎、吉田倫子、木村晴地、清流正弘、滝本晶、宿南知佐、山本照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] Runx2+/-マウスの矯正的歯の移動の牽引側における骨芽細胞の増殖および分化のRunx2/mTORC2シグナルの役割 (2017)
  • Author(s): 青沼智、玉村長都、福永智広、酒井雄一、北浦英樹、竹下信郎、山城隆、山本照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] 臼歯の挺出および下顎歯列の遠心移動により骨格的・機能的不調和を改善した骨格性Ⅲ級症例 (2017)
  • Author(s): 宮下俊郎、長谷川正和、清流正弘、山本照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] 下顎前歯先天性欠如および上顎前歯部に叢生を伴う骨格性Ⅰ級症例 (2017)
  • Author(s): 吉田倫子、清流正弘、山本照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] セルフライゲーションリンガルブラケットおよび歯科矯正用アンカースクリューの有用性 (2017)
  • Author(s): 小林 孝敬、清流 正弘、山本 照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] 片側性臼歯部crossbite患者と正常咬合者の顎関節円板位置の比較 (2017)
  • Author(s): 小林 孝敬、清流 正弘、出口 徹、長谷川 正和、山本 照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] 歯科矯正用アンカースクリューを併用して上顎骨前方牽引を行った骨格性Ⅲ級症例 (2017)
  • Author(s): 吉澤 光弘, 福永 智広, 清流 正弘, 黒木 毅, 宮島 悠旗, 山本 照子
  • Organizer: 第76回日本矯正歯科学会
• [Presentation] ラット脛骨におけるZr70Ni16Cu6Al8金属ガラス製インプラントの生体力学的、組織学的性質について (2015)
  • Author(s): 井田 裕人、清流正弘、土江雄治郎、佐々木聡史、宮下俊郎、横山嘉彦、山本照子
  • Organizer: 第74回日本矯正歯科学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] 歯科用インプラントのチタン表面処理による骨形成促進と安定性 (2015)
  • Author(s): 土江 雄治朗、清流正弘、井田裕人、佐々木聡史、佐々木周太郎、石川邦夫、都留寛治、山本照子
  • Organizer: 第74回日本矯正歯科学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] 歯科矯正用アンカースクリューを併用した上顎前方牽引装置の効果について (2015)
  • Author(s): 清流 正弘、真山敦、金原正敬、解良洋平、宮下俊郎、出口徹、山本照子
  • Organizer: 第74回日本矯正歯科学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] Clinical and Translational Dental Research focused on Orthodontic Tooth Movement. (2015)
  • Author(s): Takano-Yamamoto T
  • Organizer: 2015 Goldhaber Award Memory Lecture
  • Place of Presentation: Boston, MA, U.S.A.
  • Year and Date: 2015-10-26
  • Int'l Joint Research / Invited