| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The osteoblasts were collected from Sox21KO and WT mouse, and the expression of the bone metabolism marker was investigated by RT-PCR. The decrease of expression level was not observed in the Sox21KO mouse. Next, the effect on osteoclasts was examined. The osteoclasts differentiation potency was compared by TRAP staining from the macrophage of spleen origin, and the difference was not found in this either. It was considered indirect effect through the other cells from these results. A blood was tested since Sox21 is expressed in the blood. No changes major in the item about the bone metabolism were identified.
The osteoclast differentiation was induced from the bone marrow cell collected from the mouse femur because Sox21 was expressed in the bone marrow too. The number of osteoclasts in the Sox21KO Mouse increased compared with WT mouse.
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