Influence of chromosome 13 abnormality on hard tissue

• Project/Area Number: 15K11359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: SAITO KAN 東北大学, 大学病院, 講師 (40380852)
• Co-Investigator(Kenkyū-buntansha): 中村 卓史 東北大学, 歯学研究科, 准教授 (90585324)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯 / 発生 / Sox21 / 骨代謝 / 染色体異常 / 骨芽細胞 / 破骨細胞 / 硬組織

Outline of Final Research Achievements

The osteoblasts were collected from Sox21KO and WT mouse, and the expression of the bone metabolism marker was investigated by RT-PCR. The decrease of expression level was not observed in the Sox21KO mouse. Next, the effect on osteoclasts was examined. The osteoclasts differentiation potency was compared by TRAP staining from the macrophage of spleen origin, and the difference was not found in this either. It was considered indirect effect through the other cells from these results. A blood was tested since Sox21 is expressed in the blood. No changes major in the item about the bone metabolism were identified.
The osteoclast differentiation was induced from the bone marrow cell collected from the mouse femur because Sox21 was expressed in the bone marrow too. The number of osteoclasts in the Sox21KO Mouse increased compared with WT mouse.

Research Products

• [Int'l Joint Research] ヘルシンキ大学(フィンランド)
• [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains. (2017)
  • Author(s): Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
  • Journal Title: Sci Rep. Volume: 27 Issue: 1 Pages: 45181-45181
  • DOI: 10.1038/srep45181
  • NAID: 120006534986
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Globoside accelerates the differentiation of dental epithelial cells into ameloblasts (2016)
  • Author(s): Nakamura, T., Chiba, Y., Naruse, M., Harada, H., and Fukumoto, S.
  • Journal Title: Intenational Journal of Oral Science Volume: 8(4) Issue: 4 Pages: 205-212
  • DOI: 10.1038/ijos.2016.35
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation (2016)
  • Author(s): Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 23670-23670
  • DOI: 10.1038/srep23670
  • NAID: 120006534957
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation (2016)
  • Author(s): Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
  • Journal Title: Plos One Volume: 11 Issue: 3 Pages: 0152206-0152206
  • DOI: 10.1371/journal.pone.0152206
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation (2016)
  • Author(s): Yamada A, Futagi M, Fukumoto E, Saito K, Yoshizaki K, Ishikawa M, Arakaki M, Hino R, Sugawara Y, Ishikawa M, Naruse M, Miyazaki K, Nakamura T, Fukumoto S.
  • Journal Title: The Journal of Biological Chemistry Volume: 291 Issue: 2 Pages: 904-912
  • DOI: 10.1074/jbc.m115.674663
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Application of a tooth-surface coating material containing pre-reacted glass-ionomer fillers for caries prevention (2015)
  • Author(s): Suzuki M, Yamada A, Saito K, Hino R, Sugawara Y, Ono M, Naruse M, Arakaki M, Fukumoto S.
  • Journal Title: Pediatric Dental Journal Volume: 25 Issue: 3 Pages: 72-78
  • DOI: 10.1016/j.pdj.2015.08.003
  • Peer Reviewed / Open Access
• [Journal Article] The enamel knot-like structure is eternally maintained in the apical bud of postnatal mouse incisors. (2015)
  • Author(s): Nakatomi C, Nakatomi M, Saito K, Harada H and Ohshima H.
  • Journal Title: Archives of Oral Biology Volume: 60 Issue: 8 Pages: 1122-1130
  • DOI: 10.1016/j.archoralbio.2015.05.001
  • Peer Reviewed
• [Presentation] Sox21によるエナメル分化誘導メカニズムの解析 (2015)
  • Author(s): 齋藤 幹，福本 敏
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ (新潟)
  • Year and Date: 2015-09-11
• [Presentation] 歯の萌出時期と嚢胞形成に関わるインテグリン beta1 の分子機構について (2015)
  • Author(s): 齋藤 幹，山田亜矢，新垣真紀子，二木正晴， 中村卓史， 福本敏
  • Organizer: 第53回日本小児歯科学会
  • Place of Presentation: 広島国際会議場 (広島)
  • Year and Date: 2015-05-21
• [Remarks] 東北大学 2016年 プレスリリース
  • URL: http://www.tohoku.ac.jp/japanese/2016/04/press20160331-01.html
• [Funded Workshop] 第16回 Tooth Morphogenesis and Differentiation (2016)
  • Place of Presentation: Haikkoontie 114, 06400 Porvoo, Finland
  • Year and Date: 2016-06-12