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Elucidation of the microRNA-mediated control of the alveolar bone metabolism by PDL cells under each mechanical stresses

Research Project

Project/Area Number 15K11376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthodontics/Pediatric dentistry
Research InstitutionTsurumi University

Principal Investigator

Noda Koji  鶴見大学, 歯学部, 臨床教授 (10148059)

Co-Investigator(Kenkyū-buntansha) 宮本 豊  鶴見大学, 歯学部, 助教 (00633588)
中村 芳樹  鶴見大学, 歯学部, 教授 (10097321)
和田 悟史  鶴見大学, 歯学部, 助教 (20581119)
菅崎 弘幸  鶴見大学, 歯学部, 准教授 (30333826)
石川 美佐緒  鶴見大学, 歯学部, 助教 (90582445)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsマイクロRNA / メカニカルストレス / 歯根膜 / 矯正学的歯の移動 / 歯槽骨 / 歯周組織 / シグナル伝達 / 歯根膜細胞 / 歯の移動 / 破骨細胞 / 骨芽細胞 / 骨形成 / 骨吸収 / サイトカイン
Outline of Final Research Achievements

We aimed to elucidate the regulatory mechanism of the induction of the different bone metabolisms, bone resorption and bone formation, at the compression and tension side during orthodontic tooth movement. We focused on the microRNA (miRNA), and analysed the expression of miRNA in PDL cells under compression and tension.
We discovered that different miRNAs were upregulated in PDL cells under compression and tension. Then we examined the relationship between the change of OPG expression by different mechanical stresses and the difference of miRNA induction. miRNAs which can target OPG were firstly selected, and we found that miR-3198 was the miRNA which exhibited different change of the expression between compression and tension. miR-3198 and OPG inversely changed by compression and tension. Finally, we confirmed that miR-3198 play a role in the change of OPG expression under compression and tension.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (1 results)

  • [Journal Article] Dimethyl fumarate inhibits osteoclasts via attenuation of reactive oxygen species signaling by augmented anti oxidation2018

    • Author(s)
      Yamaguchi Y, Kanzaki H, Katsumata Y, Itohiya K, Fukaya S, Miyamoto Y, Narimiya T, Wada S, Nakamura Y
    • Journal Title

      Journal of Cellular and Molecular Medicine

      Volume: 22 Issue: 2 Pages: 1138-1147

    • DOI

      10.1111/jcmm.13367

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Possible alternative treatment for mandibular asymmetry by local unilateral igf-1 injection into the mandibular condylar cavity: Experimental study in mice.2017

    • Author(s)
      Fukaya S, Kanzaki H, Miyamoto Y, Yamaguchi Y, Nakamura Y.
    • Journal Title

      American journal of orthodontics and dentofacial orthopedics

      Volume: 152(6) Issue: 6 Pages: 820-829

    • DOI

      10.1016/j.ajodo.2017.05.023

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RANKL induces Bach1 nuclear import and attenuates Nrf2-mediated antioxidant enzymes, thereby augmenting intracellular reactive oxygen species signaling and osteoclastogenesis in mice.2017

    • Author(s)
      Kanzaki H, Shinohara F, Itohiya K, Yamaguchi Y, Katsumata Y, Matsuzawa M, Fukaya S, Miyamoto Y, Wada S, Nakamura Y
    • Journal Title

      FASEB J

      Volume: 31 Issue: 2 Pages: 781-792

    • DOI

      10.1096/fj.201600826r

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Asporin stably expressed in the surface layer of mandibular condylar cartilage and augmented in the deeper layer with age2017

    • Author(s)
      Miyamoto Y, Kanzaki H, Wada S, Tsuruoka S, Itohiya K, Kumagai K, Hamada Y, Nakamura Y
    • Journal Title

      Bone Reports

      Volume: 7 Pages: 41-50

    • DOI

      10.1016/j.bonr.2017.07.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Orthodontic tensile strain induces angiogenesis via type IV collagen degradation by matrix metalloproteinase-122017

    • Author(s)
      Narimiya, T. Wada, S. Kanzaki, H. Ishikawa, M. Tsuge, A. Yamaguchi, Y. Nakamura, Y.
    • Journal Title

      J Periodontal Res

      Volume: 印刷中 Issue: 5 Pages: 1-11

    • DOI

      10.1111/jre.12453

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel device for application of continuous mechanical tensile strain to mammalian cells2017

    • Author(s)
      Wada, Satoshi Kanzaki, Hiroyuki Narimiya, Tsuyoshi Nakamura, Yoshiki
    • Journal Title

      Biology Open

      Volume: 6 Pages: 518-524

    • DOI

      10.1242/bio.023671

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Occlusal hypofunction mediates alveolar bone apposition via relative augmentation of TGF-β signaling by decreased Asporin production in rats.2016

    • Author(s)
      Kanako Itohiya Hiroyuki Kanzaki Misao Ishikawa Satoshi Wada Yutaka Miyamoto Tsuyoshi Narimiya Yoshiki Nakamura
    • Journal Title

      Dental, Oral and Craniofacial Research

      Volume: 3 Issue: 1 Pages: 1-8

    • DOI

      10.15761/docr.1000192

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecular regulatory mechanisms of osteoclastogenesis through cytoprotective enzymes2016

    • Author(s)
      Kanzaki, H. Shinohara, F. Kanako, I. Yamaguchi, Y. Fukaya, S. Miyamoto, Y. Wada, S. Nakamura, Y.
    • Journal Title

      Redox Biol

      Volume: 8 Pages: 186-191

    • DOI

      10.1016/j.redox.2016.01.006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 矯正学的歯の移動とHMGB1 アップデートシンポジウム5「核内タンパク質HMGB1」2017

    • Author(s)
      菅崎弘幸、中村芳樹
    • Organizer
      歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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