| Project/Area Number |
15K11377
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 浩志 松本歯科大学, 歯学部, 講師 (00278178)
中道 裕子 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
宇田川 信之 松本歯科大学, 歯学部, 教授 (70245801)
大須賀 直人 松本歯科大学, 歯学部, 教授 (80247535)
溝口 利英 松本歯科大学, 総合歯科医学研究所, 講師 (90329475)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯髄細胞 / 石灰化 / 骨形成 / 破骨細胞 / 骨芽細胞 / 骨髄細胞 / 骨髄間葉細胞 / BMP-2 / アルカリホスファターゼ / オステオカルシン / リン酸トランスポーター / リバースシグナル / 石灰化メカニズム |
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| Outline of Final Research Achievements |
Dental pulp does not calcify in normal tissues. However, when dental pulp fibroblasts were removed from the body and cultured, it was found that the ability to calcify extracellular matrix produced by themselves is higher than that of osteoblasts and bone marrow mesenchymal cells. Cultured pulp fibroblasts possess osteoclastogenesis supporting ability, and the gene expression profile was very similar to osteoblasts and bone marrow mesenchymal cells. In addition, dental pulp fibroblasts were transplanted into immunodeficient mice to form bone with bone marrow. Based on the results of this research, it was possible to clarify the usefulness of pulp fibroblasts as a material in hard tissue regenerative medicine based on the molecular biological characteristics analysis result of pulp fibroblasts.
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