Effects of stem cell-derived exosomes on periodontal regeneration
| Project/Area Number |
15K11380
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Periodontology
|
|---|
| Research Institution | Kanagawa Dental College (2018)
Tokyo Medical and Dental University (2015-2017) |
|---|
Principal Investigator |
Komaki Motohiro 神奈川歯科大学, 大学院歯学研究科, 准教授 (30401368)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
岩崎 剣吾 東京医科歯科大学, 歯学部, 非常勤講師 (40401351)
森田 育男 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (60100129)
和泉 雄一 東京医科歯科大学, 歯学部, 非常勤講師 (60159803)
|
|---|
| Project Period (FY) |
2015-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 体性幹細胞 / エクソソーム / microRNA / 幹細胞 / exosome / mesenchymal stem cell / angiogenesis / Placenta / 間葉系幹細胞 / 細胞分化 / 血管新生 / micro RNA / 培養上清 |
|---|
| Outline of Final Research Achievements |
It has become clear that all the cells that make up the body, including cancer cells, secrete membrane vesicles called exosomes and exchange information between the cells. Until now, exosome research has been conducted as a new tool for cancer diagnosis. We clarified that mesenchymal stem cells secrete exosomes, which are taken up by other cells and are involved in the pluripotency and angiogenic activity that are characteristic of stem cells, and the potential for therapeutic application of exosomes are also indicated. In addition, it was shown that the function of exosomes may be due to microRNAs acting in regulation of gene expression contained in exosomes. By continuing this research, we aim to clarify the role of stem cell exosome in periodontal tissue regeneration and establish a new therapeutic method applying the exosome.
|
| Academic Significance and Societal Importance of the Research Achievements |
細胞治療は、現在、学術的にも社会的にも注目され大きな期待が寄せられている。一方で、安全性など実用化における科学的課題が残る。最近、細胞どうしがエクソソームを介して情報交換していることが明らかになった。本研究では、体性幹細胞が分泌するエクソソームの機能について検討し、体性幹細胞がエクソソームを分泌すること、分泌したエクソソームは他の細胞に取り込まれ機能することを明らかにした。この機能の一部にmRNA発現調節に関与するmicroRNAが関与する可能性を示した。歯周組織再生におけるエクソソームの役割を解明し、エクソソームにより生体の細胞を制御して再生を促す再生誘導法技術の確立を目指す。
|
Report
(5 results)
Research Products
(15 results)
-
-
-
-
[Journal Article] Cell transfer technology for tissue engineering2017
Author(s)
Akazawa Keiko、Iwasaki Kengo、Nagata Mizuki、Yokoyama Naoki、Ayame Hirohito、Yamaki Kazumasa、Tanaka Yuichi、Honda Izumi、Morioka Chikako、Kimura Tsuyoshi、Komaki Motohiro、Kishida Akio、Izumi Yuichi、Morita Ikuo
-
Journal Title
Inflammation and Regeneration
Volume: 37
Issue: 1
Pages: 21-21
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
[Presentation] Mesenchymal stem cell-derived extracellular vesicles alter differentiation competence of fibroblasts2017
Author(s)
KOMAKI, M, TOOI, M) YOKOYAMA, N, AYAME, H, IWASAKI, K, IZUMI, Y, MORITA, I
Organizer
International Society for Extracellular Vesicles 2017- Annual meeting
Related Report
Int'l Joint Research
-
-
-
-
-
[Presentation] 血管新生に対する胎盤由来間葉系幹細胞培養上清の効果2015
Author(s)
小牧基浩, 岩﨑剣吾, 沼田友里, 遠井政行, 赤澤惠子, 永田 瑞, 森丘千夏子, 本多 泉, 横山尚毅, 菖蒲弘人, 和泉雄一, 森田育男
Organizer
日本歯周病学会
Place of Presentation
幕張メッセ、千葉市、千葉
Year and Date
2015-05-15
Related Report
-
-
-
