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Effects of cathepsin A on the regeneration of periodontal ligament tissue

Research Project

Project/Area Number 15K11385
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontology
Research InstitutionOsaka University

Principal Investigator

Kitagaki Jirouta  大阪大学, 歯学部附属病院, 助教 (90570292)

Co-Investigator(Kenkyū-buntansha) 山田 聡  東北大学, 歯学研究科, 教授 (40359849)
山下 元三  大阪大学, 歯学部附属病院, 講師 (90524984)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsカテプシンA / Ebelactone B / 歯根膜細胞 / 歯周組織再生 / ゲノムワイドアプローチ
Outline of Final Research Achievements

The purpose of this study is to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.
Mouse periodontal ligament cells (MPDL22) were incubated with cathepsin A inhibitor Ebelactone B and calcification-related gene expression was assessed by real-time PCR. As a result, Ebelactone B clearly inhibited the expression of calcification-related genes such as osteopontin and type I collagen. We next evaluated genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population through exome sequencing. One of a single nucleotide polymorphism (SNP) in cathepsin A, rs181943893, showed significant different minor allele frequency (MAF) through a case-control exome sequencing.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (17 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Effects of paraoxonase 1 on the cytodifferentiation and mineralization of periodontal ligament cells.2017

    • Author(s)
      R Masumoto, J Kitagaki, M Matsumoto, S Miyauchi, C Fujihara, M Yamashita, S Yamada, M Kitamura, S Murakami.
    • Journal Title

      J Periodont Res

      Volume: 53(2) Issue: 2 Pages: 200-209

    • DOI

      10.1111/jre.12507

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of genetic risk factors of aggressive periodontitis in a Japanese population by exome sequencing2017

    • Author(s)
      北垣次郎太、枡本梨沙、宮内静香、藤原千春、村上伸也
    • Journal Title

      Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

      Volume: 59 Issue: 1 Pages: 1-9

    • DOI

      10.2329/perio.59.1

    • NAID

      130006825064

    • ISSN
      0385-0110, 1880-408X
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin Phosphodiesterase 3 Enhances Cytodifferentiation of Periodontal Ligament Cells2017

    • Author(s)
      S Miyauchi, J Kitagaki, R Masumoto, A Imai, K Kobayashi, A Nakaya, S Kawai, C Fujihara, Y Asano, M Yamashita, M Yanagita, S Yamada, M Kitamura, S. Murakami
    • Journal Title

      J Dent Res

      Volume: 96 Issue: 3 Pages: 339-46

    • DOI

      10.1177/0022034516677938

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population2016

    • Author(s)
      Kitagaki J, Miyauchi S, Asano Y, Imai A, Kawai S, Michikami I, Yamashita M, Yamada S, Kitamura M, Murakami S
    • Journal Title

      PLoS One

      Volume: 11 Issue: 8 Pages: e0160765-e0160765

    • DOI

      10.1371/journal.pone.0160765

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] エクソーム解析を用いた日本人侵襲性歯周炎の疾患関連遺伝子探索2017

    • Author(s)
      北垣次郎太、枡本梨沙、宮内静香、藤原千春、山田聡、村上伸也
    • Organizer
      第5回NGS現場の会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 歯根膜細胞の骨芽細胞への分化におけるparaoxonase 1の役割2017

    • Author(s)
      北垣 次郎太、枡本 梨沙、松本 昌大、宮内 静香、藤原 千春、山下 元三、山田 聡、北村 正博、村上 伸也
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Exome Sequencing of Aggressive Periodontitis in a Japanese Population2017

    • Author(s)
      Shizuka Miyauchi, Jirouta Kitagaki, Risa Masumoto, Motozo Yamashita, Manabu Yanagita, Satoru Yamada, Masahiro Kitamura, Shinya Murakami
    • Organizer
      2017 IADR GENERAL SESSION
    • Place of Presentation
      San Francisco, California, USA
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genome-wide Association Study of Aggressive Periodontitis in a Japanese Population2016

    • Author(s)
      Shizuka MIYAUCHI, Jirouta KITAGAKI, Motozo YAMASHITA, Satoru YAMADA, Masahiro KITAMURA, Shinya MURAKAMI
    • Organizer
      2016 IADR GENERAL SESSION
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索2016

    • Author(s)
      宮内静香、北垣次郎太、山下元三、山田聡、北村正博、村上伸也
    • Organizer
      第144回日本歯科保存学会2016年度春季学術大会
    • Place of Presentation
      栃木県総合文化センター(栃木県宇都宮市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析2016

    • Author(s)
      北垣次郎太、宮内静香、村上伸也
    • Organizer
      NGS現場の会 第4回研究会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 歯根膜細胞の骨芽細胞分化に対するSMPD3の効果2016

    • Author(s)
      北垣次郎太、宮内静香、枡本梨沙、藤原千春、山下元三、柳田学、山田聡、北村正博、村上伸也
    • Organizer
      第145回日本歯科保存学会2016年度秋季学術大会
    • Place of Presentation
      キッセイ文化ホール(長野県松本市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 歯根膜細胞の骨芽細胞分化におけるSMPD3の影響2016

    • Author(s)
      北垣次郎太、宮内静香、桝本梨沙、今井敦子、小林香織、中谷明弘、河合伸治、藤原千春、山下元三、柳田学、山田聡、北村正博、村上伸也
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of genetic risk factor for Japanese aggressive periodontitis by genome-wide association study2015

    • Author(s)
      Jirouta KITAGAKI, Shizuka MIYAUCHI, Motozo YAMASHIYA, Satoru YAMADA, Masahiro KITAMURA, Shinya MURAKAMI
    • Organizer
      International Symposium 2015 Oral and Craniofacial Development and Diseases
    • Place of Presentation
      弓倉記念ホール(大阪大学)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索2015

    • Author(s)
      北垣次郎太、宮内静香、今井敦子、朝野仁裕、河合伸治、道上郁美、山下元三、山田聡、北村正博、村上伸也
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析2015

    • Author(s)
      北垣次郎太、宮内静香、山下元三、山田聡、北村正博、村上伸也
    • Organizer
      第143回秋季保存学術大会
    • Place of Presentation
      文京シビックホール(東京)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析2015

    • Author(s)
      北垣次郎太、宮内静香、村上伸也
    • Organizer
      NGS現場の会 第4回研究会
    • Place of Presentation
      つくば国際会議場(茨城)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
  • [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索2015

    • Author(s)
      宮内静香、北垣次郎太、山下元三、山田聡、北村正博、村上伸也
    • Organizer
      第142回日本歯科保存学会2015年度春季学術大会
    • Place of Presentation
      北九州国際会議場(小倉)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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