Effects of cathepsin A on the regeneration of periodontal ligament tissue

• Project/Area Number: 15K11385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Osaka University
• Principal Investigator: Kitagaki Jirouta 大阪大学, 歯学部附属病院, 助教 (90570292)
• Co-Investigator(Kenkyū-buntansha): 山田 聡 東北大学, 歯学研究科, 教授 (40359849) ; 山下 元三 大阪大学, 歯学部附属病院, 講師 (90524984)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: カテプシンA / Ebelactone B / 歯根膜細胞 / 歯周組織再生 / ゲノムワイドアプローチ

Outline of Final Research Achievements

The purpose of this study is to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.
Mouse periodontal ligament cells (MPDL22) were incubated with cathepsin A inhibitor Ebelactone B and calcification-related gene expression was assessed by real-time PCR. As a result, Ebelactone B clearly inhibited the expression of calcification-related genes such as osteopontin and type I collagen. We next evaluated genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population through exome sequencing. One of a single nucleotide polymorphism (SNP) in cathepsin A, rs181943893, showed significant different minor allele frequency (MAF) through a case-control exome sequencing.

Research Products

• [Journal Article] Effects of paraoxonase 1 on the cytodifferentiation and mineralization of periodontal ligament cells. (2017)
  • Author(s): R Masumoto, J Kitagaki, M Matsumoto, S Miyauchi, C Fujihara, M Yamashita, S Yamada, M Kitamura, S Murakami.
  • Journal Title: J Periodont Res Volume: 53(2) Issue: 2 Pages: 200-209
  • DOI: 10.1111/jre.12507
  • Peer Reviewed / Open Access
• [Journal Article] Identification of genetic risk factors of aggressive periodontitis in a Japanese population by exome sequencing (2017)
  • Author(s): 北垣次郎太、枡本梨沙、宮内静香、藤原千春、村上伸也
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 59 Issue: 1 Pages: 1-9
  • DOI: 10.2329/perio.59.1
  • NAID: 130006825064
  • ISSN: 0385-0110, 1880-408X
  • Peer Reviewed
• [Journal Article] Sphingomyelin Phosphodiesterase 3 Enhances Cytodifferentiation of Periodontal Ligament Cells (2017)
  • Author(s): S Miyauchi, J Kitagaki, R Masumoto, A Imai, K Kobayashi, A Nakaya, S Kawai, C Fujihara, Y Asano, M Yamashita, M Yanagita, S Yamada, M Kitamura, S. Murakami
  • Journal Title: J Dent Res Volume: 96 Issue: 3 Pages: 339-46
  • DOI: 10.1177/0022034516677938
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population (2016)
  • Author(s): Kitagaki J, Miyauchi S, Asano Y, Imai A, Kawai S, Michikami I, Yamashita M, Yamada S, Kitamura M, Murakami S
  • Journal Title: PLoS One Volume: 11 Issue: 8 Pages: e0160765-e0160765
  • DOI: 10.1371/journal.pone.0160765
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] エクソーム解析を用いた日本人侵襲性歯周炎の疾患関連遺伝子探索 (2017)
  • Author(s): 北垣次郎太、枡本梨沙、宮内静香、藤原千春、山田聡、村上伸也
  • Organizer: 第5回NGS現場の会
• [Presentation] 歯根膜細胞の骨芽細胞への分化におけるparaoxonase 1の役割 (2017)
  • Author(s): 北垣 次郎太、枡本 梨沙、松本 昌大、宮内 静香、藤原 千春、山下 元三、山田 聡、北村 正博、村上 伸也
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Exome Sequencing of Aggressive Periodontitis in a Japanese Population (2017)
  • Author(s): Shizuka Miyauchi, Jirouta Kitagaki, Risa Masumoto, Motozo Yamashita, Manabu Yanagita, Satoru Yamada, Masahiro Kitamura, Shinya Murakami
  • Organizer: 2017 IADR GENERAL SESSION
  • Place of Presentation: San Francisco, California, USA
  • Int'l Joint Research
• [Presentation] Genome-wide Association Study of Aggressive Periodontitis in a Japanese Population (2016)
  • Author(s): Shizuka MIYAUCHI, Jirouta KITAGAKI, Motozo YAMASHITA, Satoru YAMADA, Masahiro KITAMURA, Shinya MURAKAMI
  • Organizer: 2016 IADR GENERAL SESSION
  • Place of Presentation: Seoul, Korea
  • Int'l Joint Research
• [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索 (2016)
  • Author(s): 宮内静香、北垣次郎太、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第144回日本歯科保存学会2016年度春季学術大会
  • Place of Presentation: 栃木県総合文化センター（栃木県宇都宮市）
• [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析 (2016)
  • Author(s): 北垣次郎太、宮内静香、村上伸也
  • Organizer: NGS現場の会 第4回研究会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
• [Presentation] 歯根膜細胞の骨芽細胞分化に対するSMPD3の効果 (2016)
  • Author(s): 北垣次郎太、宮内静香、枡本梨沙、藤原千春、山下元三、柳田学、山田聡、北村正博、村上伸也
  • Organizer: 第145回日本歯科保存学会2016年度秋季学術大会
  • Place of Presentation: キッセイ文化ホール（長野県松本市）
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるSMPD3の影響 (2016)
  • Author(s): 北垣次郎太、宮内静香、桝本梨沙、今井敦子、小林香織、中谷明弘、河合伸治、藤原千春、山下元三、柳田学、山田聡、北村正博、村上伸也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] Identification of genetic risk factor for Japanese aggressive periodontitis by genome-wide association study (2015)
  • Author(s): Jirouta KITAGAKI, Shizuka MIYAUCHI, Motozo YAMASHIYA, Satoru YAMADA, Masahiro KITAMURA, Shinya MURAKAMI
  • Organizer: International Symposium 2015 Oral and Craniofacial Development and Diseases
  • Place of Presentation: 弓倉記念ホール（大阪大学）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索 (2015)
  • Author(s): 北垣次郎太、宮内静香、今井敦子、朝野仁裕、河合伸治、道上郁美、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫）
  • Year and Date: 2015-12-01
• [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析 (2015)
  • Author(s): 北垣次郎太、宮内静香、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第143回秋季保存学術大会
  • Place of Presentation: 文京シビックホール（東京）
  • Year and Date: 2015-11-13
• [Presentation] 日本人侵襲性歯周炎患者のヒトエクソーム解読と遺伝子多型解析 (2015)
  • Author(s): 北垣次郎太、宮内静香、村上伸也
  • Organizer: NGS現場の会 第４回研究会
  • Place of Presentation: つくば国際会議場（茨城）
  • Year and Date: 2015-07-02
• [Presentation] ゲノムワイドアプローチによる日本人侵襲性歯周炎の疾患関連遺伝子探索 (2015)
  • Author(s): 宮内静香、北垣次郎太、山下元三、山田聡、北村正博、村上伸也
  • Organizer: 第142回日本歯科保存学会2015年度春季学術大会
  • Place of Presentation: 北九州国際会議場（小倉）
  • Year and Date: 2015-06-26