| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The purpose of this study is to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.
Mouse periodontal ligament cells (MPDL22) were incubated with cathepsin A inhibitor Ebelactone B and calcification-related gene expression was assessed by real-time PCR. As a result, Ebelactone B clearly inhibited the expression of calcification-related genes such as osteopontin and type I collagen. We next evaluated genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population through exome sequencing. One of a single nucleotide polymorphism (SNP) in cathepsin A, rs181943893, showed significant different minor allele frequency (MAF) through a case-control exome sequencing.
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