Exploring the molecular mechanism that correlates periodontal disease with systemic disease by metabolome analysis

• Project/Area Number: 15K11386
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Osaka University
• Principal Investigator: NOZAKI Takenori 大阪大学, 歯学部附属病院, 准教授 (30263304)
• Co-Investigator(Kenkyū-buntansha): 豊田 岐聡 大阪大学, 理学研究科, 教授 (80283828) ; 馬場 健史 九州大学, 生体防御医学研究所, 教授 (10432444) ; 柳田 学 大阪大学, 歯学研究科, 招へい教員 (80379081) ; 北垣 次郎太 大阪大学, 歯学部附属病院, 助教 (90570292)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 歯周病 / 全身疾患 / メタボロミクス / リピドミクス / 肥満 / 診断 / 質量分析 / メタボローム

Outline of Final Research Achievements

The aim of this study is to clarify the molecular group that contributes to bidirectional correlation between periodontal disease and systemic disease.
In this study, we investigated the relationship between molecules that produced as the result of the metabolic fluctuation in obesity condition, and metabolites that produced at the site of periodontal disease, by metabolomic and lipidomic analysis.
As the result, it becomes clear that we can assess the metabolic fluctuation arise from obesity by detecting the metabolome and/or lipidome in the serum. Moreover, it becomes clear that the amount of metabolites produced at the site of periodontal disease change along with periodontal condition. These results suggest that these metabolites can be the key molecules that correlate between periodontal disease and systemic disease.

Research Products

• [Journal Article] Metabolomic Analysis of Gingival Crevicular Fluid Using Gas Chromatography/Mass Spectrometry (2016)
  • Author(s): Ozeki, M., Nozaki, T., Aoki, J., Bamba, T., Jensen, K.R., Murakami, S., Toyoda, M
  • Journal Title: Mass Spectrometry Volume: 5 Issue: 1 Pages: 1-6
  • DOI: 10.5702/massspectrometry.a0047
  • NAID: 130005159151
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 医療診断のための口腔内揮発性成分分析手法開発 (2018)
  • Author(s): 大須賀潤一、野崎剛徳、奥山萌恵、村上伸也、豊田岐聡
  • Organizer: 日本質量分析学会・日本プロテオーム学会 2018年合同大会
• [Presentation] メタボローム解析の歯周病診断への応用 (2018)
  • Author(s): 野崎剛徳
  • Organizer: 質量分析オープンイノベーションシンポジウム
• [Presentation] GC/MSを用いたメタボローム解析の歯周病診断への応用 (2016)
  • Author(s): 野崎剛徳, 尾関美穂, 青木順, 馬場健史, JENSEN Kirk R., 柳田学, 北垣次郎太, 北村正博, 豊田岐聡, 村上伸也
  • Organizer: 第59回秋季日本歯周病学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-10-07
• [Presentation] GC/MSによるメタボローム解析の歯周病診断への応用 (2016)
  • Author(s): 野崎剛徳
  • Organizer: ジオミクス「先端質量分析技術で切り拓く生態圏惑星科学」
  • Place of Presentation: 大阪大学会館アセンブリーホール（ 大阪府・豊中市 ）
  • Year and Date: 2016-03-31