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Growth inhibition of selected bacterial species by peptide nucleic acids for control of oral microflora

Research Project

Project/Area Number 15K11404
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontology
Research InstitutionOsaka Dental University

Principal Investigator

MAEDA Hiroshi  大阪歯科大学, 歯学部, 教授 (00274001)

Co-Investigator(Kenkyū-buntansha) 苔口 進  岡山大学, 医歯薬学総合研究科, 准教授 (10144776)
高柴 正悟  岡山大学, 医歯薬学総合研究科, 教授 (50226768)
北松 瑞生  近畿大学, 理工学部, 講師 (60379716)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords細菌叢 / ペプチド核酸 / アンチセンス / 口腔内細菌叢 / Porphyromonas gingivalis / 歯周病原細菌 / HSP60 / 口腔細菌叢
Outline of Final Research Achievements

In this research project, we aimed to inhibit the growth of selected bacterial species in oral microflora by using antisense PNA (peptide nucleic acids). By targeting the HSP and AcpP genes of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, the antisense PNAs were designed and synthesized with carrier paptide (KFFKFFKFFK). For P. gingivalis, both antisense PNAs effectively inhibited the growth. Especially, anti-HSP PNA completely inhibited the growth for 5 hours. Comparing to P. gingivalis, the inhibit effect was weak for A. actinomycetemcomitans. Anti-HSP PNA slightly inhibit the growth, while anti-AcpP PNA did not show the effect. The cause of the low effect may be due to the small uptake of the PNA in A. actinomycetemcomitans. Antisense PNA may have the potential to reduce the population of P. gingivalis in oral microflora. New designs for carrier peptide and the target genes will be required for A. actinomycetemcomitans.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (1 results)

  • [Journal Article] IS1598 (IsPg4) distributed to abscess-forming strains of Porphyromonas gingivalis may enhance virulence through upregulation of nrdD-like gene expression.2018

    • Author(s)
      Sonoi N, Maeda H, Murauchi T, Yamamoto T, Omori K, Kokeguchi S, Naruishi K, Takashiba S
    • Journal Title

      New Microbiologica

      Volume: 41 Pages: 52-60

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple and rapid detection of Rothia mucilaginosa by loop-mediated isothermal amplification method.2018

    • Author(s)
      Keisuke Nakai, Hiroshi Maeda, Hideaki Ikenaga, Norimasa Tsuji
    • Journal Title

      日本口腔検査学会雑誌

      Volume: 掲載予定

    • NAID

      120006478723

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft Genome Sequences of Aggregatibacter actinomycetemcomitans Strains 310a and 310b.2017

    • Author(s)
      May AC, Ohta H, Maeda H, Kokeguchi S, Cugini C
    • Journal Title

      Genome Announcements

      Volume: 5 Issue: 47

    • DOI

      10.1128/genomea.01282-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: a randomized double-blind clinical trial.2016

    • Author(s)
      Takeuchi-Hatanaka K, Yasuda T, Naruishi K, Katsuragi-Fuke K, Inubushi J, Ootsuki H, Maeda H, Takashiba S.
    • Journal Title

      Journal of Periodontal Research

      Volume: 51 Issue: 3 Pages: 321-31

    • DOI

      10.1111/jre.12311

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vivo Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components2016

    • Author(s)
      Masataka Yoshikawa, Hideyuki Kakigi, Ayano Miyamoto, Sadaomi Sugimoto, Keisuke Nakai, Hideaki Ikenaga, Takeshi Inamoto, Hiroshi Maeda
    • Journal Title

      Journal of Biomedical Science and Engineering

      Volume: 9 Issue: 11 Pages: 501-514

    • DOI

      10.4236/jbise.2016.911045

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Smad2 overexpression enhances adhesion of gingival epithelial cells.2016

    • Author(s)
      Hongo S, Yamamoto T, Yamashiro K, Shimoe M, Tomikawa K, Ugawa Y, Kochi S, Ideguchi H, Maeda H, Takashiba S.
    • Journal Title

      Archives of Oral Biology.

      Volume: 71 Pages: 46-53

    • DOI

      10.1016/j.archoralbio.2016.06.025

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of glucose concentrations on the pH and growth of Streptococcus mutans and Porphyromonas gingivalis2015

    • Author(s)
      Yamawaki Isao, Taguchi Yoichiro, Kato Hirohito, Okuda Makiko, Katayama Nobuhito, Hashimoto Naoki, Shida Munenori, Nishikawa Ikuo, Tamura Isao, Miyake Tatsuro, Maeda Hiroshi, Umeda Makoto
    • Journal Title

      日本歯科保存学雑誌

      Volume: 58 Pages: 510-517

    • NAID

      130005119225

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Simple and rapid detection of Rothia mucilaginosa by loop-mediated isothermal amplification method.2017

    • Author(s)
      Keisuke Nakai, Hiroshi Maeda, Hideaki Ikenaga, Norimasa Tsuji
    • Organizer
      平成29年度日本歯科保存学会春季学術大会
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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