Oral health promotion for children: evaluation of the periodontal prevention using an Artificial Mouth System

Research Project

Project/Area Number	15K11431
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Social dentistry
Research Institution	The Nippon Dental University
Principal Investigator	TANAKA TOMOKO 日本歯科大学, 生命歯学部, 准教授 (70307958)
Co-Investigator(Kenkyū-buntansha)	八重垣健日本歯科大学, 生命歯学部, 教授 (40166468)
Project Period (FY)	2015-10-21 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	バイオフィルム / Red complex / 歯周病 / オーラルヘルスプロモーション / 人工口腔装置 / 歯周疾患予防
Outline of Final Research Achievements	The purpose of this study is how to establish a biofilm forming protocal by 4 kinds of microorganisms and to develop the basic investigation model in vitro of periodontal disease. The microorganisms were Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, S.gordonii and S. mutans. We tried biofilm formation on the section of a human tooth with the TYGVS culture medium added N-acetylmuramic acid. The biofilm formation was confirmed by the SEM observation and the bacterial flora analyses. And we found that the stability of the biofilm is affected by several conditions.