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Development of the analyzing system of DNA polymorphism aiming at application to the highly degraded samples for personal identification in Forensic Odontology

Research Project

Project/Area Number 15K11455
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Social dentistry
Research InstitutionTokai University

Principal Investigator

MINAGUCHI Kiyoshi  東海大学, 医学部, 客員教授 (00133380)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsDNA多型 / 戦没者遺骨 / 高度変性資料 / ミトコンドリアDNA型 / Y染色体多型 / X染色体STR / 硬組織 / DNA抽出 / 高度変性DNA / ミトコンドリアDNA多型 / X染色体多型 / Y染色体DNA多型 / X染色体DNA多型
Outline of Final Research Achievements

This study aimed at solution of two kinds of difficulties in the DNA analysis of the dead in WWII; one of them was identification of distant blood relationship between the dead and their relatives and the other was DNA typing from highly degraded DNA samples. Following results were obtained. We established a method that can determine mtDNA types from highly degraded bone or tooth samples common to southern area. The number of phylogenetic mtDNA haplogroups lineages at the basal level in the Japanese and Malay populations was increased. We constructed a system which can evaluate the identity of Y-STRs between the dead and their distant blood relative. A method to determine Y-lineage from highly degraded samples was also developed. The number and kinds of closely-linked X-STR haplotypes were increased. These were applied to elucidate complicated cases. A method to isolate amplifiable DNA from highly degraded samples was developed and was applied in actual forensic cases.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Malaya(Malaysia)

    • Related Report
      2016 Research-status Report
  • [Journal Article] 骨様および軟組織用断片のDNA鑑定2018

    • Author(s)
      水口 清,落合恵理子、松島 裕、大澤資樹
    • Journal Title

      DNA多型

      Volume: 26

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 日本人の姓とY染色体DNA多型の関連性2018

    • Author(s)
      佐藤 栞、落合恵理子、水口 清、垣本由布、大澤資樹
    • Journal Title

      DNA多型

      Volume: 26

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 混合資料のミトコンドリアDNA鑑定における次世代シークエンサーの応用2018

    • Author(s)
      落合恵理子、松島 裕、水口 清、垣内康宏、垣本由布、大澤資樹
    • Journal Title

      DNA多型

      Volume: 26

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Population Genetics of Identifiler System in Malaysia2016

    • Author(s)
      Nakamura Y, Samejima M, Minaguchi K, Nambiar P.
    • Journal Title

      The Bulletin of Tokyo Dental College

      Volume: 57 Issue: 4 Pages: 233-239

    • DOI

      10.2209/tdcpublication.2016-1400

    • NAID

      130006837352

    • ISSN
      0040-8891
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evaluation of Y chromosomal SNP haplogrouping in the HID-Ion AmpliSeqTM Identity Panel.2016

    • Author(s)
      Ochiai E, Minaguchi K, Nambiar P, Kakimoto Y, Satoh F, Nakatome M, Miyashita K, Osawa M
    • Journal Title

      Leg Med (Tokyo)

      Volume: 22 Pages: 58-61

    • DOI

      10.1016/j.legalmed.2016.08.001

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Multiplex APLP system for haplogrouping extremely degraded East-Asian mtDNAs.2016

    • Author(s)
      Kakuda T, Shojo H, Tanaka M, Nambiar P, Minaguchi K, Umetsu K, Adachi N.
    • Journal Title

      PLoS ONE

      Volume: 11 (6) Issue: 6 Pages: e0158463-e0158463

    • DOI

      10.1371/journal.pone.0158463

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 水口 清2016

    • Author(s)
      戦没者遺骨の鑑定を目的とした複雑事例の血縁関係の証明を目指して
    • Journal Title

      DNA多型

      Volume: 24 Pages: 17-21

    • Related Report
      2016 Research-status Report
  • [Journal Article] 次世代シーケンサーを用いたY染色体ハプログループ解析(原著論文)2016

    • Author(s)
      落合 恵理子, 宮下 京子, 水口 清, 垣本 由布, 佐藤 文子, 大澤 資樹
    • Journal Title

      DNA多型

      Volume: 24 Pages: 17-21

    • Related Report
      2016 Research-status Report
  • [Journal Article] Effects of using the GlobalFiler™ multiplex system on parent–child analyses of cases with single locus inconsistency.2016

    • Author(s)
      Ochiai E, Osawa M, Tamura T, Minaguchi K, Miyashita K, Matsushima Y, Kakimoto Y, Satoh F
    • Journal Title

      Legal Medicine

      Volume: 18 Pages: 72-74

    • DOI

      10.1016/j.legalmed.2015.12.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 骨様および軟組織用断片のDNA鑑定2017

    • Author(s)
      水口 清,落合恵理子、松島 裕、大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 日本人の姓とY染色体DNA多型の関連性2017

    • Author(s)
      佐藤 栞、落合恵理子、水口 清、垣本由布、大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 混合資料のミトコンドリアDNA鑑定における次世代シークエンサーの応用2017

    • Author(s)
      落合恵理子、松島 裕、水口 清、垣内康宏、垣本由布、大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 戦没者遺骨の鑑定を目的とした複雑事例の血縁関係の証明2015

    • Author(s)
      水口 清
    • Organizer
      日本DNA多型学会 第24回学術集会
    • Place of Presentation
      岡山大学創立五十周年記念館 (岡山市、岡山県)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Construction and application of multi-plex PCR for mtDNA sequencing from highly degraded and small amounts of DNA2015

    • Author(s)
      K. Minaguchi, K. Miyashita, M. Osawa
    • Organizer
      The 26th Congress of the International Society for Forensic Genetivs
    • Place of Presentation
      Auditorium Maximum of the Jagiellonian University (Krakcw, Poland)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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