Development of the analyzing system of DNA polymorphism aiming at application to the highly degraded samples for personal identification in Forensic Odontology

• Project/Area Number: 15K11455
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Tokai University
• Principal Investigator: MINAGUCHI Kiyoshi 東海大学, 医学部, 客員教授 (00133380)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: DNA多型 / 戦没者遺骨 / 高度変性資料 / ミトコンドリアDNA型 / Y染色体多型 / X染色体STR / 硬組織 / DNA抽出 / 高度変性DNA / ミトコンドリアDNA多型 / X染色体多型 / Y染色体DNA多型 / X染色体DNA多型

Outline of Final Research Achievements

This study aimed at solution of two kinds of difficulties in the DNA analysis of the dead in WWII; one of them was identification of distant blood relationship between the dead and their relatives and the other was DNA typing from highly degraded DNA samples. Following results were obtained. We established a method that can determine mtDNA types from highly degraded bone or tooth samples common to southern area. The number of phylogenetic mtDNA haplogroups lineages at the basal level in the Japanese and Malay populations was increased. We constructed a system which can evaluate the identity of Y-STRs between the dead and their distant blood relative. A method to determine Y-lineage from highly degraded samples was also developed. The number and kinds of closely-linked X-STR haplotypes were increased. These were applied to elucidate complicated cases. A method to isolate amplifiable DNA from highly degraded samples was developed and was applied in actual forensic cases.

Research Products

• [Int'l Joint Research] University of Malaya(Malaysia)
• [Journal Article] 骨様および軟組織用断片のDNA鑑定 (2018)
  • Author(s): 水口 清，落合恵理子、松島 裕、大澤資樹
  • Journal Title: DNA多型 Volume: 26
• [Journal Article] 日本人の姓とY染色体DNA多型の関連性 (2018)
  • Author(s): 佐藤 栞、落合恵理子、水口 清、垣本由布、大澤資樹
  • Journal Title: DNA多型 Volume: 26
• [Journal Article] 混合資料のミトコンドリアDNA鑑定における次世代シークエンサーの応用 (2018)
  • Author(s): 落合恵理子、松島 裕、水口 清、垣内康宏、垣本由布、大澤資樹
  • Journal Title: DNA多型 Volume: 26
• [Journal Article] Population Genetics of Identifiler System in Malaysia (2016)
  • Author(s): Nakamura Y, Samejima M, Minaguchi K, Nambiar P.
  • Journal Title: The Bulletin of Tokyo Dental College Volume: 57 Issue: 4 Pages: 233-239
  • DOI: 10.2209/tdcpublication.2016-1400
  • NAID: 130006837352
  • ISSN: 0040-8891
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of Y chromosomal SNP haplogrouping in the HID-Ion AmpliSeqTM Identity Panel. (2016)
  • Author(s): Ochiai E, Minaguchi K, Nambiar P, Kakimoto Y, Satoh F, Nakatome M, Miyashita K, Osawa M
  • Journal Title: Leg Med (Tokyo) Volume: 22 Pages: 58-61
  • DOI: 10.1016/j.legalmed.2016.08.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Multiplex APLP system for haplogrouping extremely degraded East-Asian mtDNAs. (2016)
  • Author(s): Kakuda T, Shojo H, Tanaka M, Nambiar P, Minaguchi K, Umetsu K, Adachi N.
  • Journal Title: PLoS ONE Volume: 11 (6) Issue: 6 Pages: e0158463-e0158463
  • DOI: 10.1371/journal.pone.0158463
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 水口 清 (2016)
  • Author(s): 戦没者遺骨の鑑定を目的とした複雑事例の血縁関係の証明を目指して
  • Journal Title: DNA多型 Volume: 24 Pages: 17-21
• [Journal Article] 次世代シーケンサーを用いたY染色体ハプログループ解析(原著論文) (2016)
  • Author(s): 落合 恵理子, 宮下 京子, 水口 清, 垣本 由布, 佐藤 文子, 大澤 資樹
  • Journal Title: DNA多型 Volume: 24 Pages: 17-21
• [Journal Article] Effects of using the GlobalFiler™ multiplex system on parent–child analyses of cases with single locus inconsistency. (2016)
  • Author(s): Ochiai E, Osawa M, Tamura T, Minaguchi K, Miyashita K, Matsushima Y, Kakimoto Y, Satoh F
  • Journal Title: Legal Medicine Volume: 18 Pages: 72-74
  • DOI: 10.1016/j.legalmed.2015.12.009
  • Peer Reviewed / Open Access
• [Presentation] 骨様および軟組織用断片のDNA鑑定 (2017)
  • Author(s): 水口 清，落合恵理子、松島 裕、大澤資樹
  • Organizer: 日本DNA多型学会第26回学術集会
• [Presentation] 日本人の姓とY染色体DNA多型の関連性 (2017)
  • Author(s): 佐藤 栞、落合恵理子、水口 清、垣本由布、大澤資樹
  • Organizer: 日本DNA多型学会第26回学術集会
• [Presentation] 混合資料のミトコンドリアDNA鑑定における次世代シークエンサーの応用 (2017)
  • Author(s): 落合恵理子、松島 裕、水口 清、垣内康宏、垣本由布、大澤資樹
  • Organizer: 日本DNA多型学会第26回学術集会
• [Presentation] 戦没者遺骨の鑑定を目的とした複雑事例の血縁関係の証明 (2015)
  • Author(s): 水口 清
  • Organizer: 日本DNA多型学会 第24回学術集会
  • Place of Presentation: 岡山大学創立五十周年記念館 (岡山市、岡山県）
  • Year and Date: 2015-11-19
  • Invited
• [Presentation] Construction and application of multi-plex PCR for mtDNA sequencing from highly degraded and small amounts of DNA (2015)
  • Author(s): K. Minaguchi, K. Miyashita, M. Osawa
  • Organizer: The 26th Congress of the International Society for Forensic Genetivs
  • Place of Presentation: Auditorium Maximum of the Jagiellonian University (Krakcw, Poland)
  • Year and Date: 2015-08-31
  • Int'l Joint Research