Analysis of a gravity-sensing pathway using Arabidopsis multiple mutants
Project/Area Number |
15K11913
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
宇宙生命科学
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Research Institution | Tohoku University |
Principal Investigator |
FUJII Nobuharu 東北大学, 生命科学研究科, 准教授 (70272002)
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Project Period (FY) |
2015-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 重力感受 / 植物 / LAZY1 / 重力 / アミロプラスト / 突然変異体 |
Outline of Final Research Achievements |
It has been believed that LAZY1 proteins play an important role on sense of gravity in plants. However, the molecular functions of LAZY1 proteins have not been revealed. In barley, agravitropic serpentina mutant were isolated by screening 600,000 M3 plants mutagenized by gamma ray. In this study, a missense mutation in HvLAZY1 gene was found in serpentina mutant. Furthermore, the linkage analysis using by 78 F2 plants that were crossed between WT (Chikurin-ibaraki No. 1) and serpentina mutant indicated that the missense mutation cosegregated with agravitropisc phenotype, suggesting a possibility that the missense mutation caused serpentina mutation.
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Academic Significance and Societal Importance of the Research Achievements |
植物の重力感受機構におけるLAZY1タンパク質の分子機能については、未解明の重要な研究課題となっている。LAZY1遺伝子の欠損が重力屈性の異常を引き起こすことが、イネ、シロイヌナズナ、トウモロコシ、タルウマゴヤシで示されているが、いずれも遺伝子欠損変異、T-DNA/transposon挿入変異、フレームシフト変異であり、遺伝子産物の機能に重要なアミノ酸配列に関する知見は得られていない。従って、本研究により見出したオオムギのHvLAZY1遺伝子のミスセンス変異はLAZY1タンパク質の分子的特性を明らかにするための知見になると期待される。
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy.2016
Author(s)
Yamazaki C, Fujii N, Miyazawa Y, Kamada M, Kasahara H, Osada I, Shimazu T, Fusejima Y, Higashibata A, Yamazaki T, Ishioka N, Takahashi H.
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Journal Title
npj Microgravity
Volume: 2
Issue: 1
Pages: 16030-16030
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] A Pathway that Laterally Transports Auxin from the Upper Side to the Lower Side of the Transition Zone of Cucumber Seedlings via Endodermal Layers is Formed Due to Gravitstimulation2016
Author(s)
Nobuharu Fujii, Chiaki Yamazaki, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi
Organizer
11th Asian Microgravity Symposium
Place of Presentation
Hokkaido University (Hokkaido, Sapporo)
Year and Date
2016-10-25
Related Report
Int'l Joint Research
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