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Analysis of a gravity-sensing pathway using Arabidopsis multiple mutants

Research Project

Project/Area Number 15K11913
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 宇宙生命科学
Research InstitutionTohoku University

Principal Investigator

FUJII Nobuharu  東北大学, 生命科学研究科, 准教授 (70272002)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords重力感受 / 植物 / LAZY1 / 重力 / アミロプラスト / 突然変異体
Outline of Final Research Achievements

It has been believed that LAZY1 proteins play an important role on sense of gravity in plants. However, the molecular functions of LAZY1 proteins have not been revealed. In barley, agravitropic serpentina mutant were isolated by screening 600,000 M3 plants mutagenized by gamma ray. In this study, a missense mutation in HvLAZY1 gene was found in serpentina mutant. Furthermore, the linkage analysis using by 78 F2 plants that were crossed between WT (Chikurin-ibaraki No. 1) and serpentina mutant indicated that the missense mutation cosegregated with agravitropisc phenotype, suggesting a possibility that the missense mutation caused serpentina mutation.

Academic Significance and Societal Importance of the Research Achievements

植物の重力感受機構におけるLAZY1タンパク質の分子機能については、未解明の重要な研究課題となっている。LAZY1遺伝子の欠損が重力屈性の異常を引き起こすことが、イネ、シロイヌナズナ、トウモロコシ、タルウマゴヤシで示されているが、いずれも遺伝子欠損変異、T-DNA/transposon挿入変異、フレームシフト変異であり、遺伝子産物の機能に重要なアミノ酸配列に関する知見は得られていない。従って、本研究により見出したオオムギのHvLAZY1遺伝子のミスセンス変異はLAZY1タンパク質の分子的特性を明らかにするための知見になると期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy.2016

    • Author(s)
      Yamazaki C, Fujii N, Miyazawa Y, Kamada M, Kasahara H, Osada I, Shimazu T, Fusejima Y, Higashibata A, Yamazaki T, Ishioka N, Takahashi H.
    • Journal Title

      npj Microgravity

      Volume: 2 Issue: 1 Pages: 16030-16030

    • DOI

      10.1038/npjmgrav.2016.30

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gravitropic response and circumnutation in pea (Pisum sativum) seedling roots.2016

    • Author(s)
      Kim HJ, Kobayashi A, Fujii N, Miyazawa Y, Takahashi H.
    • Journal Title

      Physiol Plant.

      Volume: 157 Issue: 1 Pages: 108-118

    • DOI

      10.1111/ppl.12406

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] キュウリの芽生えの発芽時の重力条件により発現が変動する遺伝子の探索2018

    • Author(s)
      藤井伸治、宮林彩智子、小林啓恵、山崎千秋、宮沢豊、鎌田源司、笠原春夫、長田郁子、嶋津徹、東端晃、山崎丘、石岡憲昭、高橋秀幸
    • Organizer
      日本宇宙生物科学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微小重力環境がキュウリ芽生えの発生生理に与える影響2017

    • Author(s)
      藤井伸治、山崎千秋、宮沢豊、鎌田源司、笠原春夫、長田郁子、嶋津徹、伏島康男、東端晃、山崎丘、石岡憲昭、高橋秀幸
    • Organizer
      第31回宇宙環境利用シンポジウム
    • Place of Presentation
      宇宙航空研究開発機構宇宙科学研究所 相模原キャンパス (神奈川県相模原市)
    • Year and Date
      2017-01-16
    • Related Report
      2016 Research-status Report
  • [Presentation] オオムギ (Hordeum vulgare) 突然変異体を利用した植物の重力応答機構の解析2016

    • Author(s)
      宮東みのり, 藤井伸治, 小林啓恵, 高橋秀幸
    • Organizer
      東北植物学会第6回大会
    • Place of Presentation
      東北大学青葉山北キャンパス (宮城県仙台市)
    • Year and Date
      2016-12-10
    • Related Report
      2016 Research-status Report
  • [Presentation] A Pathway that Laterally Transports Auxin from the Upper Side to the Lower Side of the Transition Zone of Cucumber Seedlings via Endodermal Layers is Formed Due to Gravitstimulation2016

    • Author(s)
      Nobuharu Fujii, Chiaki Yamazaki, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi
    • Organizer
      11th Asian Microgravity Symposium
    • Place of Presentation
      Hokkaido University (Hokkaido, Sapporo)
    • Year and Date
      2016-10-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] キュウリの根の重力屈性による水分屈性の抑制機構2016

    • Author(s)
      藤井伸治、宮林彩智子、小林啓恵、高橋秀幸
    • Organizer
      日本宇宙生物科学会第30回大会
    • Place of Presentation
      愛知医科大学 (愛知県長久手市)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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